Book contents
- Frontmatter
- Contents
- Preface to the seventh edition
- List of contributors
- List of abbreviations
- 1 Basic principles
- 2 Cell culture techniques
- 3 Centrifugation
- 4 Microscopy
- 5 Molecular biology, bioinformatics and basic techniques
- 6 Recombinant DNA and genetic analysis
- 7 Immunochemical techniques
- 8 Protein structure, purification, characterisation and function analysis
- 9 Mass spectrometric techniques
- 10 Electrophoretic techniques
- 11 Chromatographic techniques
- 12 Spectroscopic techniques: I Spectrophotometric techniques
- 13 Spectroscopic techniques: II Structure and interactions
- 14 Radioisotope techniques
- 15 Enzymes
- 16 Principles of clinical biochemistry
- 17 Cell membrane receptors and cell signalling
- 18 Drug discovery and development
- Index
- Plate section
- References
This chapter is part of a book that is no longer available to purchase from Cambridge Core
4 - Microscopy
Book contents
- Frontmatter
- Contents
- Preface to the seventh edition
- List of contributors
- List of abbreviations
- 1 Basic principles
- 2 Cell culture techniques
- 3 Centrifugation
- 4 Microscopy
- 5 Molecular biology, bioinformatics and basic techniques
- 6 Recombinant DNA and genetic analysis
- 7 Immunochemical techniques
- 8 Protein structure, purification, characterisation and function analysis
- 9 Mass spectrometric techniques
- 10 Electrophoretic techniques
- 11 Chromatographic techniques
- 12 Spectroscopic techniques: I Spectrophotometric techniques
- 13 Spectroscopic techniques: II Structure and interactions
- 14 Radioisotope techniques
- 15 Enzymes
- 16 Principles of clinical biochemistry
- 17 Cell membrane receptors and cell signalling
- 18 Drug discovery and development
- Index
- Plate section
- References
Summary
INTRODUCTION
Biochemical analysis is frequently accompanied by microscopic examination of tissue, cell or organelle preparations. Such examinations are used in many different applications, for example: to evaluate the integrity of samples during an experiment; to map the fine details of the spatial distribution of macromolecules within cells; to directly measure biochemical events within living tissues.
There are two fundamentally different types of microscope: the light microscope and the electron microscope (Fig. 4.1). Light microscopes use a series of glass lenses to focus light in order to form an image whereas electron microscopes use electromagnetic lenses to focus a beam of electrons. Light microscopes are able to magnify to a maximum of approximately 1500 times whereas electron microscopes are capable of magnifying to a maximum of approximately 200 000 times.
Magnification is not the best measure of a microscope, however. Rather, resolution, the ability to distinguish between two closely spaced points in a specimen, is a much more reliable estimate of a microscope's utility. Standard light microscopes have a lateral resolution limit of about 0.5 micrometers (μm) for routine analysis. In contrast, electron microscopes have a lateral resolution of up to 1 nanometer (nm). Both living and dead specimens are viewed with a light microscope, and often in real colour, whereas only dead ones are viewed with an electron microscope, and never in real colour.
- Type
- Chapter
- Information
- Principles and Techniques of Biochemistry and Molecular Biology , pp. 100 - 137Publisher: Cambridge University PressPrint publication year: 2010
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