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7 - Diagnosis of HIV-1 infection in children

from Part II - General issues in the care of pediatric HIV patients

Published online by Cambridge University Press:  03 February 2010

By
Paul Krogstad
Edited by
Steven L. Zeichner  and
Jennifer S. Read
Paul Krogstad
Affiliation:
Associate Professor, Departments of Pediatrics and Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, University of California, Los Angeles, CA
Steven L. Zeichner
Affiliation:
National Cancer Institute, Bethesda, Maryland
Jennifer S. Read
Affiliation:
National Cancer Institute, Bethesda, Maryland
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Summary

Effective management of pediatric HIV-1 infection begins with timely and accurate diagnosis. In infants, early diagnosis is essential. Life-threatening immunodeficiency can develop rapidly and unpredictably, and there are no laboratory or clinical characteristics that accurately predict rapid or slow disease progression [1]. Studies in adults and children have shown that very early treatment can slow the progression of immunodeficiency and preserve HIV-1-specific immune responses. Early detection of HIV-1 infection among pregnant women is necessary to optimize medical care for the HIV-1-infected woman and to prevent mother-to-child transmission of HIV-1. This chapter outlines the use of serology, virus culture, and molecular diagnostic methods to detect HIV-1 infection in children.

HIV-1 diagnostic assays

Detection of antibodies to HIV-1

In 1985, enzyme-linked immunosorbent (ELISA) and immunoblot (Western blot) assays were licensed in the USA for detection of HIV-1-specific IgG antibodies in serum. While other tests are now available, they remain the mainstay for serological diagnosis of infection.

A large number of ELISA-based testing kits for antibody detection of HIV-1 specific antibodies are currently licensed by the US Food and Drug Administration. Early examples employed lysates from HIV-1-infected cell culture to provide HIV-1 antigen material. More recently developed assays often use a mixture of recombinant proteins, and some contain antigens that permit detection of antibodies to HIV-2 as well as antibody responses to HIV-1 (see below).

Type
Chapter
Information
Textbook of Pediatric HIV Care , pp. 105 - 110
Publisher: Cambridge University Press
Print publication year: 2005

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References

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  • Diagnosis of HIV-1 infection in children
    • By Paul Krogstad, Associate Professor, Departments of Pediatrics and Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, University of California, Los Angeles, CA
  • Edited by Steven L. Zeichner, National Cancer Institute, Bethesda, Maryland, Jennifer S. Read, National Cancer Institute, Bethesda, Maryland
  • Book: Textbook of Pediatric HIV Care
  • Online publication: 03 February 2010
  • Chapter DOI: https://doi.org/10.1017/CBO9780511544798.010
Available formats
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To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

  • Diagnosis of HIV-1 infection in children
    • By Paul Krogstad, Associate Professor, Departments of Pediatrics and Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, University of California, Los Angeles, CA
  • Edited by Steven L. Zeichner, National Cancer Institute, Bethesda, Maryland, Jennifer S. Read, National Cancer Institute, Bethesda, Maryland
  • Book: Textbook of Pediatric HIV Care
  • Online publication: 03 February 2010
  • Chapter DOI: https://doi.org/10.1017/CBO9780511544798.010
Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

  • Diagnosis of HIV-1 infection in children
    • By Paul Krogstad, Associate Professor, Departments of Pediatrics and Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, University of California, Los Angeles, CA
  • Edited by Steven L. Zeichner, National Cancer Institute, Bethesda, Maryland, Jennifer S. Read, National Cancer Institute, Bethesda, Maryland
  • Book: Textbook of Pediatric HIV Care
  • Online publication: 03 February 2010
  • Chapter DOI: https://doi.org/10.1017/CBO9780511544798.010
Available formats
×