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Chapter 6 - Laser-Assisted ICSI

Published online by Cambridge University Press:  02 December 2021

By
Roberta Maggiulli ,
Simona Alfano ,
Erminia Alviggi ,
Gemma Fabozzi ,
Danilo Cimadomo ,
Filippo Maria Ubaldi  and
Laura Rienzi
Edited by
Gianpiero D. Palermo  and
Zsolt Peter Nagy
Gianpiero D. Palermo
Affiliation:
Cornell Institute of Reproductive Medicine, New York
Zsolt Peter Nagy
Affiliation:
Reproductive Biology Associates, Atlanta, GA
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Summary

ICSI is a well-established insemination method in IVF due to its overall efficacy and reliability, even when severe male factor is not the main cause of infertility. Although conventional-ICSI provides high fertilization rate, it suffers from some technical drawbacks. Sudden or delayed oocyte degeneration may occur after ICSI even when performed by experienced embryologist and extreme care is applied during the injection phase. Preliminary results from studies conducted in early 2000s suggest that laser-assisted micro-drilling of the zona pellucida before injection (LA-ICSI) may be useful to make ICSI more successful when inseminating oocytes more prone to fertilization failure. Although this condition does not affect many couples, in case of degeneration of all the injected oocytes the IVF cycle must be cancelled. In these cases, LA-ICSI becomes a valuable option that deserves consideration.

Keywords

In vitro fertilizationintracytoplasmic sperm injectionlaser-assisted ICSIzona pellucidametaphase II oocyteoocyte qualityfailed fertilizationdegenerationembryosfragile oocytes.
Type
Chapter
Information
Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction
With Other Advanced Micromanipulation Techniques to Edit the Genetic and Cytoplasmic Content of the Oocyte
 , pp. 59 - 65
Publisher: Cambridge University Press
Print publication year: 2021

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