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Chapter 25 - Sperm Selection in the Laboratory

from Section 4 - Laboratory Evaluation and Treatment of Male Infertility

Published online by Cambridge University Press:  06 December 2023

By
Denny Sakkas  and
Denis A. Vaughan
Edited by
Douglas T. Carrell ,
Alexander W. Pastuszak  and
James M. Hotaling
Douglas T. Carrell
Affiliation:
Utah Center for Reproductive Medicine
Alexander W. Pastuszak
Affiliation:
University of Utah
James M. Hotaling
Affiliation:
Utah Center for Reproductive Medicine
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Summary

In nature varying strategies are used to ensure that the best sperm arrives at the egg. Strategies to select the best sperm are far less rigorous in the setting of in vitro fertilization. In this chapter we discuss how in vitro sperm selection strategies utilize different components of the spermatozoon including their motility characteristics, size, membrane properties, and nuclear structure. Sperm selection strategies have included microfluidics, spectroscopy, label-free imaging, hyaluronan binding, and separation of sperm by charge. Although some studies have shown that sperm selection can improve embryo euploidy rates and pregnancy outcomes, in particular by reducing miscarriages, large clinical trials, focused on the male population who would benefit most from sperm selection, are still needed.

Keywords

male infertilitysperm selectionARTICSIIVFreproduction
Type
Chapter
Information
Men's Reproductive and Sexual Health Throughout the Lifespan
An Integrated Approach to Fertility, Sexual Function, and Vitality
 , pp. 197 - 203
Publisher: Cambridge University Press
Print publication year: 2023

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References

Lehtonen, J, Parker, GA. Gamete competition, gamete limitation, and the evolution of the two sexes. Mol Hum Reprod. 2014;20(12):1161–1168.Google Scholar
Nascimento, JM, Shi, LZ, Meyers, S, et al. The use of optical tweezers to study sperm competition and motility in primates. J R Soc Interface. 2008;5(20):297–302.Google Scholar
Sakkas, D, Ramalingam, M, Garrido, N, Barratt, CL. Sperm selection in natural conception: what can we learn from Mother Nature to improve assisted reproduction outcomes? Hum Reprod Update. 2015;21(6):711–726.Google Scholar
Katz, DF, Drobnis, EZ, Overstreet, JW. Factors regulating mammalian sperm migration through the female reproductive tract and oocyte vestments. Gamete Res. 1989;22(4):443–469.Google Scholar
Wolf, DP, Blasco, L, Khan, MA, Litt, M. Human cervical mucus. IV. Viscoelasticity and sperm penetrability during the ovulatory menstrual cycle. Fertil Steril. 1978;30(2):163–169.CrossRefGoogle ScholarPubMed
Khandwala, YS, Baker, VL, Shaw, GM, Stevenson, DK, Lu, Y, Eisenberg, ML. Association of paternal age with perinatal outcomes between 2007 and 2016 in the United States: population based cohort study. BMJ. 2018;363:k4372.Google Scholar
Evenson, DP, Darzynkiewicz, Z, Melamed, MR. Relation of mammalian sperm chromatin heterogeneity to fertility. Science. 1980;210(4474):1131–1133.Google Scholar
Evenson, D, Darzynkiewicz, Z, Jost, L, Janca, F, Ballachey, B. Changes in accessibility of DNA to various fluorochromes during spermatogenesis. Cytometry. 1986;7(1):45–53.Google Scholar
Bianchi, PG, Manicardi, GC, Bizzaro, D, Bianchi, U, Sakkas, D. Effect of deoxyribonucleic acid protamination on fluorochrome staining and in situ nick-translation of murine and human mature spermatozoa. Biol Reprod. 1993;49(5):1083–1088.Google Scholar
Hughes, CM, Lewis, SE, McKelvey-Martin, VJ, Thompson, W. A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay. Mol Hum Reprod. 1996;2(8):613–619.CrossRefGoogle ScholarPubMed
Sakkas, D, Seli, E, Manicardi, GC, Nijs, M, Ombelet, W, Bizzaro, D. The presence of abnormal spermatozoa in the ejaculate: did apoptosis fail? Hum Fertil (Camb). 2004;7(2):99–103.Google Scholar
Oehninger, S, Morshedi, M, Weng, SL, Taylor, S, Duran, H, Beebe, S. Presence and significance of somatic cell apoptosis markers in human ejaculated spermatozoa. Reprod Biomed Online. 2003;7(4):469–476.CrossRefGoogle ScholarPubMed
Jodar, M, Selvaraju, S, Sendler, E, Diamond, MP, Krawetz, SA. The presence, role and clinical use of spermatozoal RNAs. Hum Reprod Update. 2013;19(6):604–624.Google Scholar
Cayli, S, Jakab, A, Ovari, L, et al. Biochemical markers of sperm function: male fertility and sperm selection for ICSI. Reprod Biomed Online. 2003;7(4):462–468.Google Scholar
Yagci, A, Murk, W, Stronk, J, Huszar, G. Spermatozoa bound to solid state hyaluronic acid show chromatin structure with high DNA chain integrity: an acridine orange fluorescence study. J Androl. 2010;31(6):566–572.Google Scholar
Burl, RB, Clough, S, Sendler, E, Estill, M, Krawetz, SA. Sperm RNA elements as markers of health. Syst Biol Reprod Med. 2018;64(1):25–38.Google Scholar
Manicardi, GC, Tombacco, A, Bizzaro, D, Bianchi, U, Bianchi, PG, Sakkas, D. DNA strand breaks in ejaculated human spermatozoa: comparison of susceptibility to the nick translation and terminal transferase assays. Histochem J. 1998;30(1):33–39.CrossRefGoogle Scholar
Manicardi, GC, Bianchi, PG, Pantano, S, et al. Presence of endogenous nicks in DNA of ejaculated human spermatozoa and its relationship to chromomycin A3 accessibility. Biol Reprod. 1995;52(4):864–867.CrossRefGoogle ScholarPubMed
Varghese, AC, Bragais, FM, Mukhopadhyay, D, et al. Human sperm DNA integrity in normal and abnormal semen samples and its correlation with sperm characteristics. Andrologia. 2009;41(4):207–215.Google Scholar
Aitken, RJ, De Iuliis, GN. On the possible origins of DNA damage in human spermatozoa. Mol Hum Reprod. 2010;16(1):3–13.Google Scholar
Sakkas, D, Seli, E, Bizzaro, D, Tarozzi, N, Manicardi, GC. Abnormal spermatozoa in the ejaculate: abortive apoptosis and faulty nuclear remodelling during spermatogenesis. Reprod Biomed Online. 2003;7(4):428–432.Google Scholar
Said, TM, Agarwal, A, Zborowski, M, Grunewald, S, Glander, HJ, Paasch, U. Utility of magnetic cell separation as a molecular sperm preparation technique. J Androl. 2008;29(2):134–142.Google Scholar
Lee, TH, Liu, CH, Shih, YT, et al. Magnetic-activated cell sorting for sperm preparation reduces spermatozoa with apoptotic markers and improves the acrosome reaction in couples with unexplained infertility. Hum Reprod. 2010;25(4):839–846.Google Scholar
Lepine, S, McDowell, S, Searle, LM, Kroon, B, Glujovsky, D, Yazdani, A. Advanced sperm selection techniques for assisted reproduction. Cochrane Database Syst Rev. 2019;7(7):CD010461.Google Scholar
Jakab, A, Sakkas, D, Delpiano, E, et al. Intracytoplasmic sperm injection: a novel selection method for sperm with normal frequency of chromosomal aneuploidies. Fertil Steril. 2005;84(6):1665–1673.Google Scholar
Sati, L, Huszar, G. Methodology of aniline blue staining of chromatin and the assessment of the associated nuclear and cytoplasmic attributes in human sperm. Methods Mol Biol. 2013;927:425–436.Google Scholar
Huszar, G, Ozkavukcu, S, Jakab, A, Celik-Ozenci, C, Sati, GL, Cayli, S. Hyaluronic acid binding ability of human sperm reflects cellular maturity and fertilizing potential: selection of sperm for intracytoplasmic sperm injection. Curr Opin Obstet Gynecol. 2006;18(3):260–267.Google Scholar
Ovári, L, Sati, L, Stronk, J, Borsos, A, Ward, DC, Huszar, G. Double probing individual human spermatozoa: aniline blue staining for persistent histones and fluorescence in situ hybridization for aneuploidies. Fertil Steril. 2010;93(7):2255–2261.Google Scholar
Worrilow, KC, Eid, S, Woodhouse, D, et al. Use of hyaluronan in the selection of sperm for intracytoplasmic sperm injection (ICSI): significant improvement in clinical outcomes – multicenter, double-blinded and randomized controlled trial. Hum Reprod. 2013;28(2):306–314.Google Scholar
Miller, D, Pavitt, S, Sharma, V, et al. Physiological, hyaluronan-selected intracytoplasmic sperm injection for infertility treatment (HABSelect): a parallel, two-group, randomised trial. Lancet. 2019;393(10170):416–422.Google Scholar
Tarozzi, N, Nadalini, M, Bizzaro, D, et al. Sperm-hyaluronan-binding assay: clinical value in conventional IVF under Italian law. Reprod Biomed Online. 2009;19(Suppl. 3):35–43.Google Scholar
Parmegiani, L, Cognigni, GE, Bernardi, S, et al. Comparison of two ready-to-use systems designed for sperm-hyaluronic acid binding selection before intracytoplasmic sperm injection: PICSI vs. Sperm Slow: a prospective, randomized trial. Fertil Steril. 2012;98(3):632–637.Google Scholar
Majumdar, G, Majumdar, A. A prospective randomized study to evaluate the effect of hyaluronic acid sperm selection on the intracytoplasmic sperm injection outcome of patients with unexplained infertility having normal semen parameters. J Assist Reprod Genet. 2013;30(11):1471–1475.Google Scholar
McDowell, S, Kroon, B, Ford, E, Hook, Y, Glujovsky, D, Yazdani, A. Advanced sperm selection techniques for assisted reproduction. Cochrane Database Syst Rev. 2014(10):CD010461.Google Scholar
Hasanen, E, Elqusi, K, ElTanbouly, S, et al. PICSI vs. MACS for abnormal sperm DNA fragmentation ICSI cases: a prospective randomized trial. J Assist Reprod Genet. 2020;37(10):2605–2613.CrossRefGoogle ScholarPubMed
Schuster, TG, Cho, B, Keller, LM, Takayama, S, Smith, GD. Isolation of motile spermatozoa from semen samples using microfluidics. Reprod Biomed Online. 2003;7(1):75–81.Google Scholar
Smith, GD, Takayama, S. Application of microfluidic technologies to human assisted reproduction. Mol Hum Reprod. 2017;23(4):257–268.Google Scholar
Riordon, J, Tarlan, F, You, JB, et al. Two-dimensional planar swimming selects for high DNA integrity sperm. Lab Chip. 2019;19(13):2161–2167.Google Scholar
Quinn, MM, Jalalian, L, Ribeiro, S, et al. Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples. Hum Reprod. 2018;33(8):1388–1393.Google Scholar
Shirota, K, Yotsumoto, F, Itoh, H, et al. Separation efficiency of a microfluidic sperm sorter to minimize sperm DNA damage. Fertil Steril. 2016;105(2):315–321 e1.Google Scholar
Gode, F, Gürbüz, AS, Tamer, B, Pala, I, Isik, AZ. The effects of microfluidic sperm sorting, density gradient and swim-up methods on semen oxidation reduction potential. Urol J. 2020;17(4):397–401.Google ScholarPubMed
Yildiz, K, Yuksel, S. Use of microfluidic sperm extraction chips as an alternative method in patients with recurrent in vitro fertilisation failure. J Assist Reprod Genet. 2019;36(7):1423–1429.CrossRefGoogle ScholarPubMed
Parrella, A, Keating, D, Cheung, S, et al. A treatment approach for couples with disrupted sperm DNA integrity and recurrent ART failure. J Assist Reprod Genet. 2019;36(10):2057–2066.Google Scholar
Parrella, A, Tavares, RS, Haddad, M, et al. A novel method to attenuate embryo aneuploidy due to paternal inheritance. Fertil Steril. 2020;114(3):e424–e425.CrossRefGoogle Scholar
Chan, PJ, Jacobson, JD, Corselli, JU, Patton, WC. A simple zeta method for sperm selection based on membrane charge. Fertil Steril. 2006;85(2):481–486.Google Scholar
Kheirollahi-Kouhestani, M, Razavi, S, Tavalaee, M, et al. Selection of sperm based on combined density gradient and Zeta method may improve ICSI outcome. Hum Reprod. 2009;24(10):2409–2416.Google Scholar
Simon, L, Ge, SQ, Carrell, DT. Sperm selection based on electrostatic charge. Methods Mol Biol. 2013;927:269–278.Google Scholar
Ainsworth, C, Nixon, B, Aitken, RJ. Development of a novel electrophoretic system for the isolation of human spermatozoa. Hum Reprod. 2005;20(8):2261–2270.Google Scholar
Ainsworth, C, Nixon, B, Jansen, RP, Aitken, RJ. First recorded pregnancy and normal birth after ICSI using electrophoretically isolated spermatozoa. Hum Reprod. 2007;22(1):197–220.Google Scholar
Fleming, SD, Ilad, RS, Griffin, AM, et al. Prospective controlled trial of an electrophoretic method of sperm preparation for assisted reproduction: comparison with density gradient centrifugation. Hum Reprod. 2008;23(12):2646–2651.Google Scholar
Tran, D, Cooke, S, Illingworth, PJ, Gardner, DK. Deep learning as a predictive tool for fetal heart pregnancy following time-lapse incubation and blastocyst transfer. Hum Reprod. 2019;34(6):1011–1018.CrossRefGoogle ScholarPubMed
Zaninovic, N, Rosenwaks, Z. Artificial intelligence in human in vitro fertilization and embryology. Fertil Steril. 2020;114(5):914–920.Google Scholar
Butola, A, Popova, D, Prasad, DK, et al. High spatially sensitive quantitative phase imaging assisted with deep neural network for classification of human spermatozoa under stressed condition. Sci Rep. 2020;10(1):13118.Google Scholar
Kandel, ME, Rubessa, M, He, YR, et al. Reproductive outcomes predicted by phase imaging with computational specificity of spermatozoon ultrastructure. Proc Natl Acad Sci U S A. 2020;117(31):18302–18309.Google Scholar
Kovac, JR, Smith, RP, Cajipe, M, Lamb, DJ, Lipshultz, LI. Men with a complete absence of normal sperm morphology exhibit high rates of success without assisted reproduction. Asian J Androl. 2017;19(1):39–42.Google Scholar
Bartoov, B, Berkovitz, A, Eltes, F. Selection of spermatozoa with normal nuclei to improve the pregnancy rate with intracytoplasmic sperm injection. N Engl J Med. 2001;345(14):1067–1068.Google Scholar
Antinori, M, Licata, E, Dani, G, et al. Intracytoplasmic morphologically selected sperm injection: a prospective randomized trial. Reprod Biomed Online. 2008;16(6):835–841.Google Scholar
Setti, SA, Ferreira, RC, Braga, DPAF, Figueira, RCS, Iaconelli, A Jr, Borges, E Jr. Intracytoplasmic sperm injection outcome versus intracytoplasmic morphologically selected sperm injection outcome: a meta-analysis. Reprod Biomed Online. 2010;21(4):450–455.Google Scholar
Teixeira, DM, Hadyme Miyague, A, Barbosa, MA, et al. Regular (ICSI) versus ultra-high magnification (IMSI) sperm selection for assisted reproduction. Cochrane Database Syst Rev. 2020;2(2):CD010167.Google Scholar
Mallidis, C, Sanchez, V, Wistuba, J, et al. Raman microspectroscopy: shining a new light on reproductive medicine. Hum Reprod Update. 2014;20(3):403–414.CrossRefGoogle ScholarPubMed
Huser, T, Orme, CA, Hollars, CW, Corzett, MH, Balhorn, R. Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells. J Biophotonics. 2009;2(5):322–332.Google Scholar
Da Costa, R, Amaral, S, Redmann, K, Kliesch, S, Schlatt, S. Spectral features of nuclear DNA in human sperm assessed by Raman microspectroscopy: effects of UV-irradiation and hydration. PLoS ONE. 2018;13(11):e0207786.Google Scholar
De Angelis, A, Ferrara, MA, Coppola, G, et al. Combined Raman and polarization sensitive holographic imaging for a multimodal label-free assessment of human sperm function. Sci Rep. 2019;9(1):4823.Google Scholar
Sakkas, D, Alvarez, JG. Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis. Fertil Steril. 2010;93(4):1027–1036.Google Scholar
Esteves, SC, Sánchez-Martín, F, Sánchez-Martín, P, Schneider, DT, Gosálvez, J. Comparison of reproductive outcome in oligozoospermic men with high sperm DNA fragmentation undergoing intracytoplasmic sperm injection with ejaculated and testicular sperm. Fertil Steril. 2015;104(6):1398–1405.Google Scholar
Awaga, HA, Bosdou, JK, Goulis, DG, et al. Testicular versus ejaculated spermatozoa for ICSI in patients without azoospermia: a systematic review. Reprod Biomed Online. 2018;37(5):573–580.Google Scholar
Gosálvez, J, González-Martínez, M, López-Fernández, C, Fernández, JL, Sánchez-Martín, P. Shorter abstinence decreases sperm deoxyribonucleic acid fragmentation in ejaculate. Fertil Steril. 2011;96(5):1083–1086.CrossRefGoogle ScholarPubMed
Scarselli, F, Casciani, V, Cursio, E, et al. Influence of human sperm origin, testicular or ejaculated, on embryo morphokinetic development. Andrologia. 2018;50(8):e13061.Google Scholar
Vaughan, DA, Sakkas, D. Sperm selection methods in the 21st century. Biol Reprod. 2019;101(6):1076–1082.Google Scholar
Albertini, DF. The problem with being choosy when it comes to sperm selection. J Assist Reprod Genet. 2019;36(7):1297–1298.Google Scholar
Henkel, R. Sperm preparation: state-of-the-art physiological aspects and application of advanced sperm preparation methods. Asian J Androl. 2012;14(2):260–269.Google Scholar

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