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Chapter 16 - Cryopreservation of Sperm for IVF

Semen Samples and Individual Sperm

from Section 3 - Gametes

Published online by Cambridge University Press:  07 August 2023

By
Nina Desai  and
Pooja Rambhia
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Cryopreservation of human spermatozoa offers male patients the option to preserve fertility prior to initiating cancer therapy, vasectomy or assisted reproductive technology (ART) treatment. Human sperm can be easily cryopreserved in liquid nitrogen and stored for years. A number of cryopreservation techniques and cryoprotectants have been investigated. To date, however, vapour-phase or programmed slow cooling using a glycerol/egg yolk buffer cryoprotectant has been the most widely applied sperm cryopreservation protocol. Sperm cryosurvival rates of 40% to 60% are generally achievable. However, in patients having low motility or low sperm number at the outset, cryopreservation and recovery of viable sperm can be more challenging. In such cases, freezing of single sperm or small numbers of sperm is emerging as a valuable tool. With this technique, intracytoplasmic sperm injection (ICSI) can be performed even in cases with only a few frozen spermatozoa.

Keywords

cryopreservationstoragesemensperm
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 121 - 129
Publisher: Cambridge University Press
Print publication year: 2023

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References

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