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The effects of dietary fish oil on chemokine secretion by murine peritoneal and spleen cells

Published online by Cambridge University Press:  12 May 2008

H. H. Arnardottir ,
D. H. Petursdottir  and
I. Hardardottir
H. H. Arnardottir
Affiliation:
Biochemistry and Molecular Biology, Faculty of Medicine, University of Iceland, 101 Reykjavik, Iceland
D. H. Petursdottir
Affiliation:
Biochemistry and Molecular Biology, Faculty of Medicine, University of Iceland, 101 Reykjavik, Iceland
I. Hardardottir
Affiliation:
Biochemistry and Molecular Biology, Faculty of Medicine, University of Iceland, 101 Reykjavik, Iceland
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Abstract

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Type
1st International Immunonutrition Workshop, Valencia, 3–5 October 2007, Valencia, Spain
Information
Proceedings of the Nutrition Society , Volume 67 , Issue OCE1: 1st International Immunonutrition Workshop, Valencia, 3–5 October 2007, Valencia, Spain , May 2008 , E7
Copyright
Copyright © The Authors 2008

Dietary fish oil, rich in n-3 PUFA, affects immune function partly by its effects on cytokine secretion by peritoneal and splenic macrophages and T-cells(Reference Calder1, Reference Petursdottir, Olafsdottir and Hardardottir2, Reference Petursdottir and Hardardottir3). The effects of dietary fish oil on secretion of chemokines by these cells have been less studied. Macrophage inflammatory protein 1α (MIP-1α) and monocyte chemotactic protein 1 (MCP-1) are chemokines that act on and are secreted by monocytes, macrophages and T-cells(Reference Rollins4). MIP-1α may promote a T-helper (Th) 1-type immune response(Reference Schrum, Probst, Fleischer and Zipfel5), whereas MCP-1 is thought to promote a Th2-type immune response(Reference Gu, Tseng, Horner, Tam, Loda and Rollins6). The aim of the present study was to determine the effects of dietary fish oil on MIP-1α and MCP-1 secretion by murine peritoneal macrophages and splenic macrophages and T-cells ex vivo.

Mice were fed diets supplemented with (g/kg) 180 fish oil+20 maize oil or 200 maize oil for 6 weeks (n 10). Resident peritoneal macrophages were stimulated with lipopolysaccharide (LPS) with or without antibodies against TNFα or IL-10. Total spleen cells were stimulated with LPS or antibodies against CD3 and CD28. Concentration of the chemokines MIP-1α and MCP-1 in the medium was measured by ELISA.

Dietary fish oil did not affect LPS-induced MIP-1α secretion but decreased LPS-induced MCP-1 secretion by resident peritoneal macrophages (329 v. 174 pg/ml respectively, P=0.01). The effect of dietary fish oil on MCP-1 secretion was not mediated by an effect on TNFα or IL-10 production as it was not affected by antibodies against TNFα or IL-10. On the other hand, dietary fish oil increased LPS-induced MIP-1α secretion by splenic macrophages (see Figure) but had little effect on MCP-1 secretion. Dietary fish oil decreased anti-CD3/anti-CD28-induced MIP-1α secretion by splenic T-cells (see Figure) but had no effect on MCP-1 secretion.

These results demonstrate that dietary fish oil affects chemokine secretion by peritoneal and splenic macrophages as well as T-cells and that its effects depend on the cell type and location. These effects of dietary fish oil on chemokine secretion may help explain its effects on immune function in which chemokines play an important role.

References

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2. Petursdottir, DH, Olafsdottir, I & Hardardottir, I (2002) J Nutr 132, 3740–3743.CrossRefGoogle Scholar
3. Petursdottir, DH & Hardardottir, I (2007) J Nutr 137, 665–670.CrossRefGoogle Scholar
4. Rollins, BJ (1997) Blood 90, 909–928.CrossRefGoogle Scholar
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6. Gu, L, Tseng, S, Horner, RM, Tam, C, Loda, M & Rollins, BJ (2000) Nature 404, 407–411.CrossRefGoogle Scholar