Hostname: page-component-7479d7b7d-767nl Total loading time: 0 Render date: 2024-07-11T01:43:28.570Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of monolaurin on intestinal barrier, blood biochemical profile, immunity and antioxidant function in porcine epidemic diarrhoea virus-infected piglets

Published online by Cambridge University Press:  17 August 2023

Chao Wang ,
Qian Zhang Open the ORCID record for Qian Zhang [Opens in a new window] ,
Changzheng Ji ,
Yuyan Hu ,
Dan Yi ,
Tao Wu ,
Lei Wang ,
Di Zhao  and
Yongqing Hou
Chao Wang
Affiliation:
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China
Qian Zhang
Affiliation:
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China
Changzheng Ji
Affiliation:
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China
Yuyan Hu
Affiliation:
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China
Dan Yi
Affiliation:
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China
Tao Wu
Affiliation:
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China
Lei Wang
Affiliation:
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China
Di Zhao
Affiliation:
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China
Yongqing Hou*
Affiliation:
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China
*
*Corresponding author: Dr Yongqing Hou, email houyq@aliyun.com
Get access Rights & Permissions [Opens in a new window]

Abstract

The effects of monolaurin (ML) on the health of piglets infected with porcine epidemic diarrhoea virus (PEDV) have not been fully understood. This study aimed to investigate its role in blood biochemical profile, intestinal barrier function, antioxidant function and the expression of antiviral genes in piglets infected with PEDV. Thirty-two piglets were randomly divided into four groups: control group, ML group, PEDV group and ML + PEDV group. Piglets were orally administrated with ML at a dose of 100 mg/kg·BW for 7 d before PEDV infection. Results showed that PEDV infection significantly decreased D-xylose content and increased intestinal fatty acid-binding protein content, indicating that PEDV infection destroyed intestinal barrier and absorption function. While it could be repaired by ML administration. Moreover, ML administration significantly decreased plasma blood urea nitrogen and total protein content upon PEDV infection. These results suggested ML may increase protein utilisation efficiency. ML administration significantly decreased the number of large unstained cells and Hb and increased the number of leucocytes and eosinophils in the blood of PEDV-infected piglets, indicating ML could improve the immune defense function of the body. In the presence of PEDV infection, ML administration significantly increased superoxide dismutase and catalase activities in blood and colon, respectively, indicating ML could improve antioxidant capacity. Besides, ML administration reversed the expression of ISG15, IFIT3 and IL-29 throughout the small intestine and Mx1 in jejunum and ileum, indicating the body was in recovery from PEDV infection. This study suggests that ML could be used as a kind of feed additive to promote swine health upon PEDV infection.

Keywords

MonolaurinPigletsPEDVIntestineImmunityAntioxidant
Type
Research Article
Information
British Journal of Nutrition , Volume 131 , Issue 2 , 28 January 2024 , pp. 185 - 192
Check for updates
Copyright
© The Author(s), 2023. Published by Cambridge University Press on behalf of The Nutrition Society

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

These authors contributed equally to this work and should be considered co-first authors.

References

Curry, SM, Schwartz, KJ, Yoon, KJ, et al. (2017) Effects of porcine epidemic diarrhea virus infection on nursery pig intestinal function and barrier integrity. Vet Microbiol 211, 58–66.Google Scholar
Pensaert, MB, Debouck, P & Reunolds, DJ (1981) An immunoelectron microscopic and immunofluorescent study on the antigenic relationship between the coronavirus-like agent, CV777, and several coronaviruses. Arch Virol 68, 45–52.Google Scholar
Takahashi, K, Okada, K & Ohshima, KI (1983) An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan. Nihon Juigaku Zasshi 45, 829–832.Google Scholar
Kweon, CH, Kwon, BJ, Jung, TS, et al. (1993) Isolation of porcine epidemic diarrhea virus (PEDV) in Korea. Korean J Vet Res 33, 249–254.Google Scholar
Xuan, H (1984) Culture of porcine epidemic diarrhea virus by using pig intestinal monolayer cell. People’s Liberation Army Vet 4, 202–208.Google Scholar
Sun, D, Wang, X, Wei, S, et al. (2016) Epidemiology and vaccine of porcine epidemic diarrhea virus in China: a mini-review. J Vet Med Sci 78, 355–363.Google Scholar
Harrison, L, Balan, K & Babu, U (2013) Dietary fatty acids and immune response to food-borne bacterial infections. Nutrients 5, 1801–1822.Google Scholar
Meale, SJ, Chaves, AV, He, ML, et al. (2015) Effects of various dietary lipid additives on lamb performance, carcass characteristics, adipose tissue fatty acid composition, and wool characteristics. J Anim Sci 93, 3110–3120.Google Scholar
Schlievert, PM & Peterson, ML (2012) Glycerol monolaurate antibacterial activity in broth and biofilm cultures. PloS One 7, e40350.Google Scholar
Lan, J, Chen, G, Cao, G, et al. (2021) Effects of α-glyceryl monolaurate on growth, immune function,volatile fatty acids, and gut microbiota in broiler chickens. Poult Sci 100, 100875.Google Scholar
Thormar, H, Isaacs, CE, Brown, HR, et al. (1987) Inactivation of enveloped viruses and killing of cells by fatty acids and monoglycerides. Antimicrob Agents Chemother 31, 27–31.Google Scholar
Li, Q, Estes, JD, Schlievert, PM, et al. (2009) Glycerol monolaurate prevents mucosal SIV transmission. Nature 458, 1034–1038.Google Scholar
Zhao, MJ, Cai, HY, Liu, MY, et al. (2020) Effects of dietary glycerol monolaurate on productive performance, egg quality, serum biochemical indices, and intestinal morphology of laying hens. Zhejiang Univ Sci B 21, 342.Google Scholar
Zhang, Q, Yi, D, Ji, C, et al. (2022) Monolaurin confers a protective effect against porcine epidemic diarrhea virus infection in piglets by regulating the interferon pathway. Front Immunol 12, 797476.Google Scholar
Hou, YQ, Wang, L, Ding, BY, et al. (2010) Dietary α-ketoglutarate supplementation ameliorates intestinal injury in lipopolysaccharide-challenged piglets. Amino Acids 39, 555–564.Google Scholar
Tan, B, Li, XG, Kong, XF, et al. (2009) Dietary l-arginine supplementation enhances the immune status in early-weaned piglets. Amino Acids 37, 323–331.Google Scholar
Wang, JJ, Chen, LX, Li, DF, et al. (2008) Intrauterine growth restriction affects the proteomes of the small intestine, liver and skeletal muscle in newborn pigs. J Nutr 138, 60–66.Google Scholar
Li, P, Kim, SW, Li, XL, et al. (2009) Dietary supplementation with cholesterol and docosahexaenoic acid affects concentrations of amino acids in tissues of young pigs. Amino Acids 37, 709–716.Google Scholar
Nofrarıás, M, Manzanilla, EG, Pujols, J, et al. (2006) Effects of spray-dried porcine plasma and plant extracts on intestinal morphology and on leukocyte cell subsets of weaned pigs. J Anim Sci 84, 2735–2742.Google Scholar
Hou, Y, Wang, L, Yi, D, et al. (2014) Dietary supplementation with tributyrin alleviates intestinal injury in piglets challenged with intrarectal administration of acetic acid. Br J Nutr 111, 1748–1758.Google Scholar
Jung, K & Saif, LJ (2015) Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunoprophylaxis. Vet J 204, 134–143.Google Scholar
Kuzmuk, KN & Schook, LB (2011) Pigs as a model for biomedical sciences. The genetics of the pig: second edition. CABI Publishing 2, 426–444.Google Scholar
Mansoori, B, Nodeh, H, Modirsanei, M, et al. (2009) D-Xylose absorption test: a tool for the assessment of the effect of anticoccidials on the intestinal absorptive capacity of broilers during experimental coccidiosis. Anim Feed Sci Tech 148, 301–308.Google Scholar
Luk, GD, Bayless, TM & Baylin, SB (1983) Plasma postheparin diamine oxidase, Sensitive provocative test for quantitating length of acute intestinal mucosal injury in the rat. J Clin Invest 71, 1308–1315.Google Scholar
Kralisch, S, Klöting, N, Ebert, T, et al. (2015) Circulating adipocyte fatty acid-binding protein induces insulin resistance in mice in vivo. Obesity 23, 1007–1013.Google Scholar
Zhang, Q, Wu, T, Li, S, et al. (2021) Protective effect of zinc oxide and its association with neutrophil degranulation in piglets infected with porcine epidemic diarrhea virus. Oxid Med Cell Longev 23, 3055810.Google Scholar
Kong, L, Wang, Z, Xiao, C, et al. (2022) Glycerol monolaurate attenuated immunological stress and intestinal mucosal injury by regulating the gut microbiota and activating AMPK/Nrf2 signaling pathway in lipopolysaccharide-challenged broilers. Anim Nutr 10, 347–359.Google Scholar
Zhang, X, Zhao, Q, Ci, X, et al. (2020) Evaluation of the efficacy of chlorogenic acid in reducing small intestine injury, oxidative stress, and inflammation in chickens challenged with clostridium perfringens type A. Poult Sci 99, 6606–6618.Google Scholar
Zhao, X, Wang, Y, Liu, C, et al. (2020) Prognostic value of total bilirubin in patients with st-segment elevation acute myocardial infarction undergoing primary coronary intervention. Front Cardiovasc Med 7, 615254.Google Scholar
Liu, YY, Kong, XF, Jiang, GL, et al. (2015) Effects of dietary protein/energy ratio on growth performance, carcass trait, meat quality, and plasma metabolites in pigs of different genotypes. J Anim Sci Biotechnol 6, 36.Google Scholar
Coma, J, Zimmerman, DR & Carrion, D (1995) Relationship of rate of lean tissue growth and other factors to concentration of urea in plasma of pigs. J Anim Sci 73, 3649–3656.Google Scholar
Wu, T, Lv, Y, Li, X, et al. (2018) Establishment of a recombinant escherichia coli-induced piglet diarrhea model. Front Biosci (Landmark Ed) 23, 1517–1534.Google Scholar
Yu, BP (1994) Cellular defenses against damage from reactive oxygen species. Physiol Rev 74, 139–162.Google Scholar
Li, Q, Liu, Y, Che, Z, et al. (2012) Dietary L-arginine supplementation alleviates liver injury caused by escherichia coli LPS in weaned pigs. Innate Immun 18, 804–814.Google Scholar
Jung, K, Eyerly, B, Annamalai, T, et al. (2015) Structural alteration of tight and adherens junctions in villous and crypt epithelium of the small and large intestine of conventional nursing piglets infected with porcine epidemic diarrhea virus. Vet Microbiol 177, 373–378.Google Scholar
Jinghui, F & Yijing, L (2005) Cloning and sequence analysis of the M gene of porcine epidemic diarrhea virus LJB/03. Virus Genes 30, 69–73.Google Scholar
Engelhardt, OG, Sirma, H, Pandolfi, PP, et al. (2004) Mx1 GTPase accumulates in distinct nuclear domains and inhibits influenza A virus in cells that lack promyelocytic leukaemia protein nuclear bodies. J Gen Virol 85, 2315–2326.Google Scholar
Spence, JS, He, R, Hoffmann, HH, et al. (2019) IFITM3 directly engages and shuttles incoming virus particles to lysosomes. Nat Chem Biol 15, 259–268.Google Scholar
Shi, HX, Yang, K, Liu, X, et al. (2010) Positive regulation of interferon regulatory factor 3 activation by Herc5 via ISG15 modification. Mol Cell Biol 30, 2424–2436.Google Scholar
Meager, A, Heath, A, Dilger, P, et al. (2014) Participants of the collaborative study, standardization of human IL-29 (IFN-λ1): establishment of a World Health Organization international reference reagent for IL-29 (IFN-λ1). J Interferon Cytokine Res 34, 876–884.Google Scholar