Skip to main content Accessibility help
×
Home

A Seed Bioassay to Identify Acetyl-CoA Carboxylase Inhibitor Resistant Wild Oat (Avena fatua) Populations

  • Bruce G. Murray (a1), Lyle F. Friesen (a1), Kelly J. Beaulieu (a1) and Ian N. Morrison (a1)

Abstract

A seed bioassay was developed and tested for the rapid identification of aryloxyphenoxypropionate (APP) and cyclohexanedione (CHD) resistance in wild oat. Two susceptible (S) genotypes, UM5 and Dumont, were treated with fenoxaprop-P and sethoxydim over a range of dosages on filter paper and agar. The former is a wild oat line and the latter a tame oat cultivar. Within 5 d, shoot and root development of both genotypes were completely inhibited by 10 μM fenoxaprop-P and 5 μM sethoxydim. These dosages were then tested to determine if they were suitable for distinguishing between resistant (R) and susceptible (S) plants. Agar medium was preferred over filter paper because of the ease of preparation and maintenance. Four known R wild oat populations were included in the tests. Those with high levels of resistance produced significantly longer coleoptiles and roots than S genotypes, but those with moderate or low levels of resistance could not be separated statistically from S biotypes based on quantitative measurements. However, after exposing the germinating, treated seeds to light for 24 to 48 h, all the R populations produced green coleoptiles and initiated a first leaf, unlike the S genotypes which did not turn green or produce any new growth. This procedure proved useful in discriminating between R and S genotypes and in ranking populations in terms of relative levels of resistance.

Copyright

References

Hide All
1. Beckie, H. J., Friesen, L. F., Nawolsky, K. M., and Morrison, I. N. 1990. A rapid bioassay to detect trifluralin-resistant green foxtail (Setaria viridis). Weed Technol. 4:505508.
2. Brain, P. and Cousens, R., 1989. An equation to describe dose responses where there is stimulation of growth at low doses. Weed Res. 29:9396.
3. Freund, R. J. and Littell, R. C. 1986. p. 133141 in SAS System for Regression. SAS Inst. Inc., Cary, NC.
4. Gomez, K. A. and Gomez, A. A. 1984. p. 187207 in Statistical Procedures for Agricultural Research. 2nd ed. John Wiley & Sons, New York.
5. Heap, I. M. 1994. Identification and documentation of herbicide resistance. Phytoprotection 75(Suppl.)8590.
6. Heap, I. M. and Morrison, I. N. 1996. Resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides in green foxtail (Setaria viridis). Weed Sci. (in press).
7. Heap, I. M., Murray, B. G., Loeppky, H. A., and Morrison, I. N. 1993. Resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides in wild oat (Avena fatua). Weed Sci. 41:232238.
8. Koutsoyiannis, A. 1977. p. 8191 in Theory of Econometrics. 2nd ed. MacMillan Education Ltd., London, U.K.
9. Morrison, I. N. and Devine, M. D. 1994. Herbicide resistance in the Canadian prairie provinces: Five years after the fact. Phytoprotection 75(Suppl.): 516.
10. Weed Science Society of America. 1994. Herbicide Handbook. 7th ed. Weed Sci. Soc. Am., Champaign, IL. p. 266268.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed