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Novel Use of Trinexapac-Ethyl to Study Weed Seed Germination

Published online by Cambridge University Press:  20 January 2017

Héctor Roberto Huarte ,
Antonio Jesús Ruiz Carmona  and
María Luz Zapiola
Héctor Roberto Huarte*
Affiliation:
Department of Crop Sciences, School of Agricultural Sciences, Universidad Católica Argentina (UCA)
Antonio Jesús Ruiz Carmona
Affiliation:
Department of Crop Sciences, School of Agricultural Sciences, Universidad Católica Argentina (UCA)
María Luz Zapiola
Affiliation:
Department of Crop Sciences, School of Agricultural Sciences, Universidad Católica Argentina (UCA)
*
Corresponding author's E-mail: robertohuarte@uca.edu.ar.
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Abstract

Understanding seed biology and onset of germination requirements is a key point for designing effective weed management programs. Gibberellins (GAs) are known to play a role in onset of germination of several species. Onset of germination often requires an increase in de novo GA content or an increase in sensitivity to GAs. Reduced germination of seeds incubated in solutions containing compounds that inhibit GA synthesis provide evidence that GAs are required to trigger germination. Trinexapac-ethyl (TE), a GA synthesis inhibitor, is frequently used as a plant growth regulator in crop production. However, to the best of our knowledge, TE has not been used to study the requirement of GAs in onset of germination. Germination studies were conducted using seeds of artichoke thistle and common teasel under a range of TE concentrations (0 to 500 μM TE), a combined solution of 125 μM TE + 100 μM GA3 for artichoke, and 250 μM TE + 100 μM GA3 for common teasel. Germination tests were conducted at 20/10 C (12-h thermoperiod) in darkness for artichoke and at constant 15 C with 12 h of light for common teasel. Germination of artichoke in 125 μM TE was reduced to 47% when compared with the check (95%), but the combined TE + GA3 solution maintained germination at 84%. Germination of common teasel in 250 μM TE was reduced to 10% when compared with the check (91%), and the combined TE + GA3 solution increased germination to 63%. These results demonstrate the utility of TE to study the role of GAs in onset of germination. This novel use of TE is a valuable option to study germination requirements of weed species, and therefore contributes to the design of weed management programs.

El entender la biología de las semillas y los requisitos para la germinación son puntos clave para el diseño de programas de manejo de malezas efectivos. Se sabe que gibberellins (GAs) juegan un papel en el inicio de la germinación en varias especies. El inicio de la germinación a menudo requiere de un aumento en el contenido de GA de novo o un aumento en la sensibilidad a GAs. Germinación reducida de semillas incubadas en soluciones que contienen compuestos que inhiben la síntesis de GA brindan evidencia de que GAs son requeridas para promover la germinación. Trinexapac-ethyl (TE) es un inhibidor de la síntesis de GA que es frecuentemente usado como regulador del crecimiento vegetal en la producción de cultivos. Sin embargo, hasta donde conocemos, TE no ha sido usado para el estudio del requisito de GAs en el inicio de la germinación. Se realizaron estudios de germinación usando semillas de Cynara cardunculus y Dipsacus fullonum con una variedad de concentraciones de TE (0 a 500 μM TE), una solución combinada de 125 μM TE + 100 μM GA3 para C. cardunculus, y 250 μM TE + 100 μM GA3 para D. fullonum. Las pruebas de germinación se realizaron a 20/10 C (período termal de 12-h) en oscuridad para C. cardunculus y a 15 C constantes con 12 h de luz para D. fullonum. La germinación de C. cardunculus en 125 μM TE se redujo a 47% al compararse con el testigo (95%), pero la solución combinada de TE + GA3 mantuvo la germinación a 84%. La germinación de D. fullonum en 250 μM TE se redujo a 10% en comparación con el testigo (91%), y la solución combinada de TE + GA3 aumento la germinación a 63%. Este uso novedoso de TE es una opción valiosa para el estudio de los requisitos de germinación de especies de malezas, y por lo tanto contribuye al diseño de programas de manejo de malezas.

Keywords

Gibberellinstrinexapac-ethylprohexadione-calciumartichoke thistle, Cynara cardunculus L. CYCAcommon teasel, Dipsacus fullonum L. DIFU2Dormancygerminationplant growth regulatorsseed biologyweed biologyweed management
Type
Research Article
Information
Weed Technology , Volume 28 , Issue 4 , December 2014 , pp. 729 - 733
Copyright
Copyright © Weed Science Society of America 

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