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Metabolic profiling of glyphosate-resistant sourgrass (Digitaria insularis)

Published online by Cambridge University Press:  05 March 2020

Tiago Gazola Open the ORCID record for Tiago Gazola [Opens in a new window] ,
Leandro Bianchi ,
Márcio Furriela Dias Open the ORCID record for Márcio Furriela Dias [Opens in a new window] ,
Caio A. Carbonari Open the ORCID record for Caio A. Carbonari [Opens in a new window]  and
Edivaldo D. Velini Open the ORCID record for Edivaldo D. Velini [Opens in a new window]
Tiago Gazola
Affiliation:
Ph.D. Student, Department of Plant Protection, Paulista State University, São Paulo, Brazil
Leandro Bianchi
Affiliation:
Ph.D. Student, Department of Plant Protection, Paulista State University, São Paulo, Brazil
Márcio Furriela Dias
Affiliation:
Ph.D. Student, Department of Plant Protection, Paulista State University, São Paulo, Brazil
Caio A. Carbonari*
Affiliation:
Associate Professor, Department of Production and Plant Breeding, Paulista State University, São Paulo, Brazil
Edivaldo D. Velini
Affiliation:
Professor, Department of Production and Plant Breeding, Paulista State University, São Paulo, Brazil
*
Author for correspondence: Caio Antonio Carbonari, Department of Production and Plant Breeding, Paulista State University, University Avenue 3780, Botucatu, São Paulo, Brazil. Email: carbonari@fca.unesp.br
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Abstract

Putative glyphosate-resistant sourgrass was collected to determine its resistance level and to evaluate its metabolic profile after resistance. Although accumulation of shikimic acid is known to occur in glyphosate-susceptible populations, differences in the ability of resistant (R) and susceptible (S) biotypes to accumulate quinic acid, salicylic acid, and aminomethylphosphonic acid (AMPA) have been studied to a lesser extent. Our objective was to confirm glyphosate resistance in sourgrass and to understand the metabolic profile of these plants in response to the herbicide. Greenhouse experiments were carried out from January 2016 to June 2018. There were no significant differences in glyphosate translocation in the plants. No metabolism of glyphosate to AMPA was observed; therefore, metabolism of glyphosate to AMPA is not a mechanism in R biotypes. S biotypes showed higher concentrations of shikimic acid and quinic acid before glyphosate and accumulated less of both secondary acids in treated leaves 72 h after glyphosate application. Resistant biotypes showed higher concentrations of salicylic acid before glyphosate application.

Keywords

AMPAEPSPSherbicide resistancequinic acidsalicylic acidshikimic acidGlyphosatesourgrass, Digitaria insularis (L.) Mez ex Ekman
Type
Research Article
Information
Weed Technology , Volume 34 , Issue 5 , October 2020 , pp. 748 - 755
Copyright
© Weed Science Society of America, 2020

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Footnotes

Associate Editor: Scott McElroy, Auburn University

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