Field research was conducted from 1987 to 1991 to evaluate the influence of four weed management systems on weed population and species dynamics in conventional-tillage and no-tillage grain sorghum production. These weed management systems included zero, low, medium, and high input systems. The weed seedbank increased faster in zero and low input weed management systems than in the high input weed management systems because of differences in weed control. Tillage influenced weed seed densities as well. Common ragweed, common lambsquarters, horseweed, and sicklepod seed densities often were greater in no-tillage than conventional-tillage plots. Common cocklebur and large crabgrass seed densities were usually greater in conventional-tillage than no-tillage plots. Smooth pigweed seed densities were not affected by tillage. Increasing weed management inputs diminished differences between tillage systems. Smooth pigweed dominated the weed populations after 4 yr in both tillage systems even in high input systems.

Effects of bearded sprangletop interference durations on Lemont and Newbonnet rice cultivars were studied. Interference durations of 63, 70, and 130 d after rice emergence reduced Lemont grain yields 11, 21, and 50%, respectively, and lowered Newbonnet grain yields 11, 13, and 37%, respectively. Interference durations of 21 to 56 d after emergence did not reduce grain yields of either cultivar. Bearded sprangletop grown in Lemont rice produced more biomass than that in Newbonnet. Season-long interference reduced plant height and straw dry weight of Lemont more than that of Newbonnet.

Studies were conducted to determine the effects of scarification, temperature, osmotic potential, and pH on seed germination of buffalo gourd and to examine seedling emergence and establishment. Mechanical and chemical scarification did not increase germination compared to non-scarified seed. Seed germinated at constant temperatures in the dark from 15 to 37 C with optimum germination at 25 C. As osmotic potentials became more negative from −0.1 to −0.8 MPa, germination decreased from 77 to 53%. Germination increased from 15% at pH 2.2 to 90% at pH 8. Seedlings were able to emerge from depths as great as 12 cm, and plants younger than 19 d were not able to sprout from the roots when the shoots were removed.

Field experiments were conducted to determine the effect of a short-term spring-seeded smother plant on corn development and weed control. Yellow mustard was managed to provide interference durations of 2,4,6, or 8 wk, and maximum height of 10 or 20 cm. Three yellow mustard planting patterns and eight seeding rates were evaluated during 1989 and 1990 at St. Paul and Rosemount, MN. Yellow mustard seeded at 2120 seeds m−2 with an interference duration of 4 wk and a maximum height of 10 cm decreased corn yield 17% and reduced weed dry weight 4 wk after yellow mustard emergence an average of 66%. Yellow mustard with a 2-wk interference duration did not reduce weed dry weight. Yellow mustard seeded at 2120 seeds m−2 with a 6- or 8-wk life cycle and 10-cm height reduced weed dry weight at the end of the interference period an average of 82% but delayed corn silk emergence an average of 5.3 d and reduced average grain yield 19%. Increasing yellow mustard height from 10 to 20 cm delayed corn silk emergence and reduced grain yield but did not decrease weed dry weight. Yellow mustard with an interference duration of 4 wk and a maximum height of 10 cm, seeded over the corn row at 530 seeds m−2, reduced weed dry weight 4 wk after mustard emergence an average of 51%, and resulted in an average corn grain yield reduction of 4%, compared with corn grown in monoculture averaged over weedy and weed-free treatments. These results suggest that it may be possible to develop spring-seeded smother plants that reduce weed biomass up to 80% but have only a small impact on corn yield.

Kochia pollen dispersion was measured during 24 and 48 h periods from a kochia population in an 8- by 10-m area in the center of a 1.6 ha fallow field. Pollen counts from traps at 50- and 100-cm heights declined rapidly with increasing distance from the pollen source. Pollen deposition was highest along the prevailing wind direction: up to 23 pollen grains cm–2 were recovered 50 m from the pollen source along the southeast (SE) vector. Nonlinear regression analysis of pollen deposition along the SE vector was used to estimate that 99.9% of shed pollen would be deposited within 154.4 m of the source. Viability of pollen from greenhouse- and field-grown plants was measured using staining and germination assays. of four pollen stains tested, only 1,2,3-triphenyl tetrazolium chloride gave consistent results and did not stain heat-killed pollen. Depending on environmental conditions, kochia pollen remained viable from less than 1 d to 12 d. Length of kochia pollen viability was shortest under high temperatures (22 and 28 C) and low relative humidity (7 and 32%). Less than 0.5% germination was observed in 1.1% agar media with various additions; however, up to 17.8% germination was observed after incubation at 28 C in 100% relative humidity.

Alternaria crassa, a mycoherbicide for jimsonweed control, was tested for differential effects on growth, infectivity, and phenolic metabolism of two field-collected jimsonweed varieties. Seeds from field-grown red- and green-stemmed jimsonweed varieties were grown in the greenhouse and spores were applied in aqueous solutions. Infection studies showed no significant differences between the two weed varieties when tested at various growth stages from cotyledonary to 8–leaf stage at 2.5 × 104 spores ml−1. Spore dose response tests with A. crassa spores (6.25 to 100 x 103 spores ml−1) showed no significant varietal differences in disease ratings. Extractable phenylalanine ammonia-lyase activity increased nearly equally (2- to 3-fold above control) in both varieties (8- to 9-leaf stage) 48 to 72 h after treatment with 5 × 104 spores ml−1. The only significant differences between treated varieties occurred 72 h after treatment. Exposure to A. crassa lowered total ethanol-soluble phenolic levels in both varieties 72 h after treatment. Spectrophotometric scans (400 to 700 nm) of ethanol extracts (72 h after treatment) indicated that infection caused some absorbance increases, especially in red-stemmed jimsonweed. Anthocyanin content was about 11-fold higher in red-stemmed than green-stemmed jimsonweed, and A. crassa treatment slightly increased the anthocyanin absorption peak of the red-stemmed variety.

Continuous-cropping conservation tillage systems may provide a viable alternative to the practice of summer fallow; however, concerns have been raised regarding potentially negative changes in weed communities in continuous cropping. Field experiments were established in Saskatchewan at three locations to determine the nature of weed community differences between a crop sequence with and without fallow in zero-, minimum-, and conventional-tillage systems from 1986 to 1990. Weed communities in continuous-cropping treatments tended to have greater total densities and were more similar in composition than crop-fallow treatments. Inclusion or exclusion of fallow within the rotation had a greater impact on weed community composition than did tillage system at Ituna and Waldron, but the reverse was true at Tadmore due to poor crop growth in all tillage systems. Differences in weed community composition were generally characterized by fluctuational changes in species associations. Volunteers of summer-annual crops, such as canola, flax, and barley, were associated with continuous cropping, but other species including perennial weeds, such as Canada thistle, perennial sowthistle, and quackgrass, were not strongly associated with the presence or absence of fallow. The practice of fallowing land to manage weeds may not be necessary.

Studies were conducted from 1987 to 1990 to measure the dynamics of sicklepod established at subeconomic threshold populations in a peanut-cotton-corn cropping system. The experimental site had no native populations of sicklepod prior to initiation of the study. Main plots were crops in the rotation sequence plus continuous summer fallow (no crop). Subplots were: sicklepod established in the initial year of the study, sicklepod established every year of the study, and no sicklepod. Sicklepod was established at subeconomic threshold densities to simulate weed survival and seed production in fields where economic thresholds were the basis for weed management decisions. Sicklepod growing alone in fallow plots produced more seed per plant, resulting in significantly more seedlings throughout the study than sicklepod growing with crops. Sicklepod growing in corn produced the fewest seed per plant. Seed produced from subeconomic threshold densities established only in the first year caused 7-, 21-, and 20-fold increases in sicklepod populations during the next three seasons compared to the nontreated control.

Field studies were conducted from 1988 through 1990 in Fayetteville, AR, to measure competitiveness of spotted spurge in cotton. Population levels were established by transplanting seedlings from other field areas. There was a positive linear relationship between cotton boll numbers and seed cotton yield. Percent reduction in cotton height, leaf area, dry weight, boll numbers, and seed cotton yield increased as spotted spurge densities increased. Seed cotton yield reduction was 47, 57, and 85% for spotted spurge densities of 5, 10, and 50 spotted spurge plants m–1 of row.

In response to farmer complaints of poor triallate performance, wild oat seed was collected from 34 fields in Alberta in the fall of 1990. Screening trials in the greenhouse indicated that 15 of the populations were highly resistant to triallate applied at the equivalent of the recommended field rate (1.7 kg ha-1), whereas the other 19 populations were adequately controlled. All triallate-resistant populations were also highly resistant to difenzoquat applied at 1.7 kg ha-1 (equivalent to twice the recommended field rate). The effect of increasing rates of both herbicides on dry weight of five of the resistant and two of the susceptible populations was determined in greenhouse experiments. Triallate applied up to 3.4 kg ha-1 had little or no effect on the resistant populations, whereas the susceptible populations were controlled at 1.7 kg ha-1. At rates of 6.8 kg ha-1 or higher, there were differences among the resistant populations and among individuals within the populations in the response to triallate. Response of the resistant populations to increasing difenzoquat rates was variable between experiments, but in all cases the effect of difenzoquat on wild oat dry weight was considerably less in triallate-resistant than triallate-susceptible populations. Effects of increasing rates of triallate and difenzoquat on resistant and susceptible wild oat populations growing with barley in field experiments were generally similar to the responses in the greenhouse.

Rigid ryegrass population VLR69 has become resistant to nine classes of herbicides after 21 yr of exposure to five herbicides in five different chemical classes. The population was exposed to diuron in 17 seasons and is resistant to diuron (4 fold) and chlorotoluron (8 fold) when compared with a reference susceptible population (VLR1). VLR69 had six seasons of exposure to chlorsulfuron and exhibits a high level of resistance to chlorsulfuron (> 20 fold) and triasulfuron (> 25 fold) and a lesser change in sensitivity to sulfometuron (7 fold); however, 4% of the population has a high level of resistance to sulfometuron. Resistance to atrazine (5 fold), simazine (6 fold), and ametryn (10 fold) was observed after five seasons of exposure to atrazine. There is a high level of resistance to all aryloxyphenoxypropionate herbicides after only two exposures to diclofop eight generations prior to testing the population. The population was cross-resistant to tralkoxydim (> 9.5 fold) and sethoxydim (1.8 fold). There was a small change in sensitivity to paraquat (1.4 fold) after three generations of exposure. The population displayed cross-resistance to: imazaquin (7 fold), imazapyr (2.5 fold), metribuzin (8.7 fold), and metolachlor (2 fold) but was susceptible to oxyfluorfen and dinitroaniline herbicides. There was also a small shift in sensitivity to tridiphane (1.6 fold).

Seed germination, one aspect of fitness, of chlorsulfuron-resistant (R) and -susceptible (S) kochia biotypes collected in North Dakota and Kansas was compared at 8, 18, and 28 C. Cumulative germination, characterized for each biotype using a Weibull function, was different at 8 and 18 C but not at 28 C. The Kansas R biotype reached 50% and maximum germination 70 and 300 h before Kansas S biotype at 8 C and 12 and 100 h before the S biotype at 18 C, respectively. The North Dakota R biotype reached 50% and maximum germination 12 and 100 h before the North Dakota S biotype at 8 C, respectively, and they were not different at 18 C. The resistance trait affects the cumulative germination process of kochia and may affect resistant-weed management strategies implemented early in the growing season when temperatures are lowest.

Dormant wild oat seed require afterripening under warm-dry conditions for conversion to a nondormant state capable of germination. Research was conducted to determine the relationship between temperature and seed moisture levels on afterripening of dormant wild oat line M73 seed, and to evaluate the status of water binding in the dormant seed. Conversion of dormant wild oat seed to a nondormant state at 20 to 40 C occurs primarily in the range of 5 to 20% seed moisture. There is an inverse relationship between temperature and seed moisture content for afterripening as measured by seed germination. As the afterripening temperature increases, the seed moisture content must decrease for maximum afterripening (germination) to occur. Moisture isotherms and derived enthalpy curves indicate three regions of water binding which reflect decreased binding of water to seed components as the moisture content in the dormant seed increases. Maximum afterripening, in the second region of water binding, corresponds to seed moisture contents of 7 to 22%. In this region water is weakly associated with macromolecular surfaces and begins to have solvent properties. Because afterripening occurs mainly in the second region of water binding it is likely that individual enzymatic and nonenzymatic reactions, rather than metabolic processes, mediate the conversion of wild oat seed to the nondormant state.

Genetic variation of morphological and phenological characters of yellow nutsedge (Cyperus esculentus L.) from California was investigated and compared with isozyme data from the same populations. The importance of collection location, individual genotype, and isozyme genotype to phenotypic characteristics was evaluated. Analyses were conducted on 20 individuals collected from each of 10 widely separated populations in California. Replicate plants were started by tubers, grown in pots buried outdoors, and measured during the period from planting through flowering. Results showed that yellow nutsedge is variable across its range in California in all measured traits, including days to sprouting and flowering, height, rachis number and length, aboveground biomass, tuber weight, number of rays, ray length, number of spikelets per ray, as well as spikelet length and width. Population (collection location) was a larger variance component than genotype (individuals over all locations). As found in isozyme analysis, relatively more variation was found among than within populations, typical of clonally reproducing species. Overall, more variation was found in quantitative traits than in isozymes. The level of variation, based on coefficients of variation for each character and population, was similar within populations and not clearly related to cropping history or climate at each site. The two most common isozyme genotypes in the collection differed in four characters: date of emergence, weight of 10 tubers, rachis number, and mean ray length, which may represent different adaptive responses to cultural practices. These results indicate that isozymes do not reflect the high level of genetic and adaptive diversity of yellow nutsedge. Factors that are likely to determine the patterns of variation in this species include breeding system (vegetative reproduction), founder effects, polyploidy, and homogeneity of the agricultural environmental.

Field studies were conducted to determine the influence of phosphorus (P) fertility and method of application (banded versus broadcast) on the competitive interaction of lettuce and spiny amaranth. Weed density significantly affected lettuce head weight and spiny amaranth shoot biomass after 5 wk of interference. Weed density and duration of interference had little or no effect on P content of lettuce tissue. Duration of interference did affect P concentration of spiny amaranth; however, weed density did not Spiny amaranth competition reduced lettuce yield, but P was not the limiting factor. Duration of interference and method of P application interactively affected lettuce head weight; however, only duration of interference affected spiny amaranth biomass. Seven wk of interference caused a decrease in lettuce head weight of 20, 8, and 24% when P was broadcast, banded, or not applied, respectively. Banding of P reduced the negative impact of spiny amaranth on lettuce. Although method of P application influenced the interaction between lettuce and spiny amaranth, interspecific competition between the two species probably was not due to competition for P but some other factor.

Laboratory experiments were carried out under aseptic conditions to study the effect of nitrogen applied as ammonium nitrate at 0, 20,40,60,80 and 100 ppm (wt per vol) in either distilled H2O or modified Hoagland's solution on germination and radicle length of branched broomrape in association with growing seedlings of different crops. As nitrogen rate increased, seed germination and radicle length of branched broomrape decreased linearly in flax, lentil, pepper, tomato, and wheat in modified Hoagland's solution. Similar responses to nitrogen application were found with distilled water. The parasite's seed germination was considerably lower in the media with wheat than with other crops, and lower still in the absence of crop seedings.

Experiments were conducted to evaluate the influence of temperature and water potential on water uptake, germination, and emergence of large crabgrass in order to predict emergence in the field. Water uptake of seed soaked in polyethylene glycol solutions of 0 to −1400 kPa underwent an initial imbibition phase followed by a lag phase and subsequent increase in water content when radicles emerged from the seed. Maximum germination at 15 C was 12% at 0 kPa and 60% at 25 C at 0 to −200 kPa osmotic potential. In the growth chamber, large crabgrass emergence from soil began 2 to 3 d after planting at 30 or 35 C and within 9 to 10 d at 15 C. Maximum emergence of 77 % occurred at 25 C and at a soil water potential of −30 kPa. Emergence percentage decreased as water potential decreased or as temperature increased or decreased. A logistic equation described emergence of large crabgrass at each combination of temperature and soil water potential at which emergence occurred, and a predictive model was developed and validated by field data. In the field, there was little or no emergence at soil temperatures below 15 C or water potentials below −50 to −60 kPa. The model predicted the time of onset of large crabgrass emergence and the time to reach maximum emergence to within 2 to 4 d of that recorded in field experiments. The model also predicted the correct number of flushes of emergence occurring in the field in three of four experiments.

Separate field experiments were conducted for cotton and soybean in 1990 and 1991 to determine the influence of planting date on yield loss due to interspecific interference from entireleaf morningglory and sicklepod and to determine the relative competitiveness of each weed species. Percent soybean yield loss due to weed interference increased as planting date was delayed from early May to early June. Averaged over weed species, yield losses from 1.7 weeds m−1 row were 10, 18, and 20% for soybeans planted in early May, mid-May, and early June, respectively. Yield loss from 6.7 weeds m−1 row were 17, 31, and 35% at the early May, mid-May, and early June planting dates, respectively. Percent seed cotton yield losses averaged over weed species in 1990 were 33 and 28% for the early May and early June planting dates, respectively, at 1.7 weeds m−1 and 50% for both planting dates at weed densities of 6.7 plants m−1. The only experimental factor that significantly affected seed cotton yield in 1991 was weed density. Unlike soybeans, planting date had little effect on weed interference in cotton. Entireleaf morningglory was more competitive than sicklepod in both crops. Results suggest that selection of optimum soy bean planting dates may be a viable means of reducing losses due to weed interference.

Management of perennial weeds is a major concern in reduced-tillage cropping systems. Field research was conducted at Nashua, IA, from 1977 through 1990 to evaluate the long-term impacts of tillage and cropping patterns on perennial weed populations in corn and soybean production. Continuous corn and a corn/soybean rotation were conducted utilizing moldboard plow, chisel plow, ridge tillage, and no-tillage systems. The research area was free of established perennial weed species at the initiation of the experiment in 1977. Hemp dogbane was observed by 1980, with the greatest densities in no-tillage. By 1990, continuous corn had greater hemp dogbane densities with no-tillage than other tillage system by crop rotation treatments. American germander densities were not affected by tillage systems in 1980 and 1981, but by 1990, corn/soybean rotations had greater densities in moldboard plow than other tillage systems. Field bindweed developed primarily in the corn/soybean rotations with the greatest densities occurring in no-tillage. Greater and more diverse populations of perennial weeds developed in reduced-tillage systems than in the moldboard plow system. However, practices used to control annual weeds and environmental factors interacted with tillage to regulate perennial weed populations.

Field experiments were conducted at Vegreville, Alberta, in 1990, 1991, and 1992 to investigate the influence of canola plant density and row spacing (1991 and 1992 only) on interference from Tartary buckwheat. Nonlinear regression equations incorporating both canola and Tartary buckwheat density provided good descriptions of the data. Parameter estimates for both weed and crop density were significant (P < 0.05) but did not differ between row spacings. Where no Tartary buckwheat plants were present, canola yield was little affected by canola plant density. At a given Tartary buckwheat density, canola seed yield increased as canola plant density increased, while Tartary buckwheat shoot weight and seed yield decreased. None of the factors significantly affected canola 1000-seed weight, or oil or protein levels in the canola seed. The results suggest that seeding canola to achieve a density of approximately 200 plants m–2 will significantly reduce the impact of weeds on canola yield, as well as reduce weed biomass and seed yield.