Based on chlorophyll content, hydroponically cultured soybean seedlings were 254, 66, and 13 times more tolerant to clomazone than velvetleaf, corn, and smooth pigweed, respectively. Clomazone, at concentrations that inhibited chlorophyll, did not affect fresh weight accumulations of any species except velvetleaf. However, in velvetleaf, fresh weight accumulation was only half as sensitive to clomazone as the leaf chlorophyll content. Uptake of 14C-clomazone from nutrient solution by 72 h after treatment (HAT) (pigweed > velvetleaf > soybean > corn) indicates that differential absorption cannot account for selectivity. Shoot:root ratios of 14C recovered from soybean, corn, velvetleaf, and pigweed by 72 HAT were 0.39, 0.84, 1.67, and 2.37, respectively. The limited acropetal clomazone translocation in soybean seedlings may account to a small degree for soybean tolerance to clomazone. Conversion of clomazone to more polar metabolites was rapid in all four species. There were no significant differences among species in the percentage of 14C activity recovered as clomazone from root tissue by 72 HAT. Of the 14C activity recovered from shoots of soybean, corn, pigweed, and velvetleaf seedlings by 72 HAT, 46, 59, 35, and 54%, respectively, was clomazone. Differences in clomazone uptake, distribution, and metabolism among the four species were either insignificant or poorly correlated to selectivity, and therefore cannot account for the tremendous differences in clomazone sensitivity among these species. These observations indicate, indirectly, that differences at the site of action may account for selectivity.