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Effect of abscisic acid and slow drying on soluble carbohydrate content in developing embryoids of carrot (Daucus carota L.) and alfalfa (Medicago sativa L.)

Published online by Cambridge University Press:  19 September 2008

F. A. A. Tetteroo ,
C. Bomal ,
F. A. Hoekstra  and
C. M. Karssen
F. A. A. Tetteroo
Affiliation:
Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands Royal Sluis, Westeinde 161, 1601 BM Enkhuizen, Netherlands
C. Bomal
Affiliation:
Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands
F. A. Hoekstra*
Affiliation:
Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands
C. M. Karssen
Affiliation:
Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands
*
* Correspondence
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Abstract

In the presence of abscisic acid (ABA), contents of glucose and fructose decreased in carrot embryoids, whereas umbelliferose increased during dehydration. The acquisition of desiccation tolerance was tested by scoring germinated embryoids. It did not keep pace with the changes in soluble carbohydrate contents during development, which suggests that in these somatic embryoids di- and oligosaccharides are not the determining factors for anhydrobiosis. However, on slow dehydration, umbelliferose increased, while sucrose decreased and the monosaccharides declined completely. These changes were positively correlated with increased desiccation tolerance. A similar analysis of slowly dried, ABA-treated alfalfa embryoids showed that stachyose and sucrose increased and the monosaccharides declined with the acquisition of desiccation tolerance. These data support the contention that carbohydrates are involved in anhydrobiosis.

Keywords

abscisic acidcarbohydratesDaucus carota L.desiccation toleranceMedicago sativa L.slow dryingsomatic embryogenesis
Type
Short Communication
Information
Seed Science Research , Volume 4 , Issue 2 , June 1994 , pp. 203 - 210
Copyright
Copyright © Cambridge University Press 1994

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