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Introduction to in vivo31P magnetic resonance spectroscopy of (human) skeletal muscle

Published online by Cambridge University Press:  12 June 2007

A. Heerschap ,
C. Houtman ,
H. J. A. in 't Zandt ,
A. J. van den Bergh  and
B. Wieringa
A. Heerschap*
Affiliation:
Department of Radiology, Faculty of Medical Sciences, University of Nijmegen, 6500 HB Nijmegen, The Netherlands
C. Houtman
Affiliation:
Department of Neurology, Faculty of Medical Sciences, University of Nijmegen, 6500 HB Nijmegen, The Netherlands
H. J. A. in 't Zandt
Affiliation:
Department of Radiology, Faculty of Medical Sciences, University of Nijmegen, 6500 HB Nijmegen, The Netherlands
A. J. van den Bergh
Affiliation:
Department of Radiology, Faculty of Medical Sciences, University of Nijmegen, 6500 HB Nijmegen, The Netherlands
B. Wieringa
Affiliation:
Department of Cell Biology and Histology, Faculty of Medical Sciences, University of Nijmegen, 6500 HB Nijmegen, The Netherlands
*
*Corresponding Author: Dr A. Heerschap, fax +31 24 3540 866, email a.heerschap@rdiag.azn.nl
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Abstract

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31P magnetic resonance spectroscopy (MRS) offers a unique non-invasive window on energy metabolism in skeletal muscle, with possibilities for longitudinal studies and of obtaining important bioenergetic data continuously and with sufficient time resolution during muscle exercise. The present paper provides an introductory overview of the current status of in vivo31P MRS of skeletal muscle, focusing on human applications, but with some illustrative examples from studies on transgenic mice. Topics which are described in the present paper are the information content of the 31P magnetic resonance spectrum of skeletal muscle, some practical issues in the performance of this MRS methodology, related muscle biochemistry and the validity of interpreting results in terms of biochemical processes, the possibility of investigating reaction kinetics in vivo and some indications for fibre-type heterogeneity as seen in spectra obtained during exercise.

Keywords

31P magnetic resonance spectroscopySkeletal muscleHigh-energy phosphatesEnergy metabolismExercise
Type
Meeting Report
Information
Proceedings of the Nutrition Society , Volume 58 , Issue 4 , November 1999 , pp. 861 - 870
Copyright
The Nutrition Society

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