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Anthocyanin-rich berry-extract treatment decreases expression of dietary glucose transporter genes in human intestinal Caco-2 cells

Published online by Cambridge University Press:  26 May 2010

F. Alzaid ,
K. Pourvali ,
P. A. Sharp ,
D. Bagchi ,
V. R. Preedy  and
H. Wiseman
F. Alzaid
Affiliation:
Nutritional Sciences Division, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, UK
K. Pourvali
Affiliation:
Nutritional Sciences Division, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, UK
P. A. Sharp
Affiliation:
Nutritional Sciences Division, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, UK
D. Bagchi
Affiliation:
Department of Pharmacological and Pharmaceutical Sciences, University of Houston College of Pharmacy, Houston, TX 77204, USA
V. R. Preedy
Affiliation:
Nutritional Sciences Division, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, UK
H. Wiseman
Affiliation:
Nutritional Sciences Division, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, UK
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Abstract

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Abstract
Information
Proceedings of the Nutrition Society , Volume 69 , Issue OCE4: Winter Meeting, 15 December 2009, Food supply and quality in a climate-changed world , 2010 , E326
Copyright
Copyright © The Authors 2010

Berries are a rich source of polyphenolic compounds such as flavonoids, including anthocyanins, with wide-ranging potential therapeutic effects(Reference Zafra-Stone, Yasmin and Bagchi1). It has been demonstrated previously that intestinal uptake of dietary glucose is impeded by the presence of polyphenolic compounds; however, little is known regarding the genes involved in this mechanism(Reference Kwon, Eck and Chen2). The aim of this study was to investigate the effects of an anthocyanin-rich berry-extract on dietary glucose transporting genes in Caco-2 cells.

Human intestinal Caco-2 cells were cultured for 19 d and were then treated for 16 h with an anthocyanin-rich berry-extract (OptiBerry; InterHealth Nutraceuticals, Benicia, CA, USA) at a final concentration of 0.5% (w/v). Subsequently mRNA was isolated and used for the quantitative real-time polymerase chain reaction (qRT–PCR), using 18S and GAPDH as housekeeping genes. Gene expression data are expressed as means (±sem) relative expression ratios of control; n=5/6.

The expression of GLUT2 (apical/basolateral monosaccharide transporter), GLUT5 (apical fructose transporter) and SGLT1 (apical sodium/glucose co-transporter) was decreased by the berry-extract treatment. GLUT2, GLUT5 and SGLT1 expression as a ratio of the control was as follows: GLUT2 (0.20±0.02; P=1.9×10−4), GLUT5 (0.57±0.08; P=2.5×10−3) and SGLT1 (0.45±0.03; P=3.1×10−2).

GLUT2, GLUT5 and SGLT1 have previously been identified as therapeutic targets for dysregulated glucose metabolism. In this study, the expression of these genes in Caco-2 cells was decreased by treatment with an anthocyanin-rich berry-extract. Studies are in progress to investigate the biological relevance of the observed effects in relation to berry consumption and the absorption of dietary sugar.

References

1.Zafra-Stone, S, Yasmin, T, Bagchi, M et al. (2007) Mol Nutr Food Res 51, 675–683.CrossRefGoogle Scholar
2.Kwon, O, Eck, P, Chen, S et al. (2007) FASEB J 21, 366–377.CrossRefGoogle Scholar