Welfare is often defined as the state of an individual to cope with the environment in which it finds itself (Fraser and Broom, 1997). This statement is further endorsed by the assertion that stress is triggered by environmental stimuli that cause a defence reaction or ‘stress response’ (Mostl and Palme, 2002). Stress can be measured by alterations in behaviour and through changes in physiological parameters. In a recent report, the Farm Animal Welfare Committee (2005) postulated that elevated levels of stress in semi feral ponies at auction maybe apparent in those ponies herded through auction sale rings by handlers displaying limited competence and knowledge of equine husbandry. Aversive handling and the consequent fear that animals feel towards humans can be a major source of stress and should be considered a welfare concern (Rushen et al., 1999). Research into aversive handling has a propensity to be more focused on intensively farmed production animals. However, it is apparent that the fast movement of pony stock through sale rings, and the often aggressive manner in which this can be carried out, may not be considered optimal for the welfare of these ponies. The aim of this study was to investigate the effects of prior handling experience on the stress response of semi feral foals at auction.