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Targeted metabolite profiling and de novo transcriptome sequencing reveal the key terpene synthase genes in medicinally important plant, Couroupita guianensis Aubl

Published online by Cambridge University Press:  04 January 2024

Hirekodathakallu V. Thulasiram Open the ORCID record for Hirekodathakallu V. Thulasiram [Opens in a new window] ,
Shrikant Jagannathrao Karegaonkar ,
Poojadevi Sharma ,
Ashish Kumar ,
Sudha Ramkumar  and
Avinash Pandreka
Hirekodathakallu V. Thulasiram*
Affiliation:
Chemical Biology Unit, Division of Organic Chemistry, CSIR-National Chemical Laboratory, Pashan, Pune-411008, Maharashtra, India Academy of Scientific & Innovative Research (AcSIR), CSIR-Human Resource Development Centre Campus, Ghaziabad, Uttar Pradesh-201002, India CSIR-Institute of Genomics and Integrative Biology, New Delhi-110007, India
Shrikant Jagannathrao Karegaonkar
Affiliation:
Chemical Biology Unit, Division of Organic Chemistry, CSIR-National Chemical Laboratory, Pashan, Pune-411008, Maharashtra, India Academy of Scientific & Innovative Research (AcSIR), CSIR-Human Resource Development Centre Campus, Ghaziabad, Uttar Pradesh-201002, India
Poojadevi Sharma
Affiliation:
Chemical Biology Unit, Division of Organic Chemistry, CSIR-National Chemical Laboratory, Pashan, Pune-411008, Maharashtra, India
Ashish Kumar
Affiliation:
Chemical Biology Unit, Division of Organic Chemistry, CSIR-National Chemical Laboratory, Pashan, Pune-411008, Maharashtra, India Academy of Scientific & Innovative Research (AcSIR), CSIR-Human Resource Development Centre Campus, Ghaziabad, Uttar Pradesh-201002, India
Sudha Ramkumar
Affiliation:
Chemical Biology Unit, Division of Organic Chemistry, CSIR-National Chemical Laboratory, Pashan, Pune-411008, Maharashtra, India
Avinash Pandreka
Affiliation:
Chemical Biology Unit, Division of Organic Chemistry, CSIR-National Chemical Laboratory, Pashan, Pune-411008, Maharashtra, India
*
Corresponding author: Hirekodathakallu V. Thulasiram; Email: hv.thulasiram@ncl.res.in
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Abstract

The Lecythidaceae family tree, Couroupita guianensis Aubl, popularly known as Nagpushpa, is a widely cultivated ornamental tree with several uses in traditional medicine. The tree is revered as highly sacred in Indian traditional culture due to its uniquely shaped, fragrant flowers. Considering the significance, we were prompted to carry out the metabolite and transcriptome analysis of Nagapushpa. The flower, petals, stamen, stem and leaf of C. guianensis were metabolically profiled, and it was discovered that the flower tissue contained the highest terpenoid reservoir. A number of terpenoid pathway transcripts were also found in the flower tissue after transcriptome profiling. KEGG pathway mapping was carried out to correlate transcript sequences with the biosynthesis of different types of terpenes. We were able to clone three full-length terpene synthase gene candidates, i.e. monoterpene ocimene synthase, diterpene ent-kaurene synthase and sesquiterpene farnesene synthase. The transcript expression of selected terpene synthase genes was also verified in flower tissue. These cloned sequences were used for in silico structural investigations and protein function prediction at the level of 3D structure. The data presented in this study provide a comprehensive resource for the metabolic and transcriptomic profiles of C. guianensis. The study paves the way towards the elucidation of terpene biosynthetic pathway in C. guianensis and heterologous production of useful terpenoids in the future.

Keywords

Couroupita guianensis Aublflowermetabolite profilingterpene synthasestranscriptomics
Type
Research Article
Information
Plant Genetic Resources , Volume 21 , Issue 6 , December 2023 , pp. 558 - 570
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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Footnotes

*

Authors contributed equally.

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