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Simple sequence repeat (SSR) markers for Elymus, Pseudoroegneria and Pascopyrum species (Triticeae: Gramineae)

Published online by Cambridge University Press:  17 May 2011

I. W. Mott ,
S. R. Larson  and
B. S. Bushman
I. W. Mott*
Affiliation:
United States Department of Agriculture, Agricultural Research Service, Forage and Range Research Laboratory, Utah State University, Logan, UT84322-6300, USA
S. R. Larson
Affiliation:
United States Department of Agriculture, Agricultural Research Service, Forage and Range Research Laboratory, Utah State University, Logan, UT84322-6300, USA
B. S. Bushman
Affiliation:
United States Department of Agriculture, Agricultural Research Service, Forage and Range Research Laboratory, Utah State University, Logan, UT84322-6300, USA
*
*Corresponding author. E-mail: ivan.mott@ars.usda.gov
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Abstract

The Triticeae tribe (Poaceae) includes several important cereal crops, cultivated forages, annual and perennial grass weeds and ecologically diverse native North American grasses. Elymus L. is the largest and most complex genus in the Triticeae tribe with approximately 150 polyploid perennial grass species occurring worldwide. The genomic constitutions of approximately 40% of the Elymus species are unknown. Molecular markers are needed to facilitate genetic analysis of diversity and functional traits in these species. We have developed simple sequence repeat (SSR) markers for use in Elymus based on Elymus expressed sequence tag sequences. To test the polymorphic content and transferability of these SSRs, 100 SSR primer pairs were tested on 84 plants representing seven North American Elymus, Pseudoroegneria and Pascopyrum species. The number of bands produced from each of the SSRs ranged from 1 to 11 with an average of 4.3 bands/SSR. A subset of the 23 most polymorphic SSRs produced 142 bands, an average of 6.2 bands/SSR. Binary data from the 100 SSRs successfully separated all individuals into their respective accessions in a neighbour-joining phylogram with a 100% confidence interval. Analysis of molecular variance showed that 29.9% of the total variation was within and 70.1% was among the accessions. These SSRs will be a useful tool for investigating genetic diversity, genome constitutions and molecular mapping in Elymus and other Triticeae grasses.

Keywords

Elymusgenetic diversitymolecular markersSSR
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 4 , December 2011 , pp. 489 - 494
Copyright
Copyright © NIAB 2011

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References

Asay, KH and Jensen, KB (1996) Wildryes. Cool-season forage grasses. In: Moser, LE, Buxton, DR and Casler, MD (eds) Agronomy Monograph 34. Madison, WI: ASA-CSSA-SSSA, pp. 725–748.Google Scholar
Barkworth, ME and Dewey, DR (1985) Genomically based genera in the perennial Triticeae of North America identification and membership. American Journal of Botany 72: 767–776.Google Scholar
Benham, JJ (2001) Genographer, Version 1.6.1. Bozeman, MT: Montana State University.Google Scholar
Bushman, BS, Larson, SR, Mott, IW, Cliften, PF, Wang, RR-C, Chatterton, NJ, Hernandez, AG, Ali, S, Kim, RW, Thimmapuram, J, Gong, G, Liu, L and Mikel, MA (2008) Development and annotation of perennial Triticeae ESTs and SSR markers. Genome 51: 779–788.Google Scholar
Dewey, DR (1971) Synthetic hybrids of Hordium bogdanii with Elymus canadensis and Sitanion hystrix. American Journal of Botany 58: 902–908.Google Scholar
Dewey, DR (1975) The origin of Agropyron smithii. American Journal of Botany 62: 524–530.CrossRefGoogle Scholar
Dewey, DR (1984) The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae. In: Gustafson, JP (ed.) Gene Manipulation in Plant Improvement. New York, NY: Plenum Publishing Corporation, pp. 209–279.CrossRefGoogle Scholar
Jensen, KB and Asay, KH (1996) Cytology and morphology of Elymus hoffmanni (Poaceae: Triticeae): a new species from the Erzurum Province of Turkey. International Journal of Plant Sciences 157: 750–758.CrossRefGoogle Scholar
Jensen, KB, Zhang, YF and Dewey, DR (1990) Mode of pollination of perennial species of the Triticeae in relation to genomically defined genera. Canadian Journal of Plant Science 70: 215–225.Google Scholar
Jones, TA and Larson, SR (2005) Status and use of important native grasses adapted to sagebrush communities. In: Shaw, NL, Pellant, M, Monson, SB, comps. 2005. Sage-grouse Habitat Restoration Symposium Proceedings; 2001 June 4–7; Boise, ID. Proceedings RMRS-P-38. Fort Collins, CO: U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station, pp. 49–55.Google Scholar
Lewis, SM, Martinez, AJ and Dubcovsky, J (1996) Karyotype variation in South American Elymus (Triticeae). International Journal of Plant Sciences 157: 142–150.CrossRefGoogle Scholar
Mason-Gamer, RJ, Orme, NL and Anderson, CM (2002) Phylogenetic analysis of North American Elymus and the monogenomic Triticeae (Poaceae) using three chloroplast DNA data sets. Genome 45: 991–1002.Google Scholar
McMillan, E and Sun, G (2004) Genetic relationships of tetraploid Elymus species and their genomic donor species inferred from polymerase chain reaction restriction length polymorphism analysis of chloroplast gene regions. Theoretical and Applied Genetics 108: 535–542.CrossRefGoogle ScholarPubMed
Saha, MC, Young, CA and Hopkins, AA (2009) Genetic variation within and among wildrye (Elymus canadensis and E. virginicus) populations from the Southern Great Plains. Crop Science 49: 913–922.Google Scholar
Schneider, S, Roessli, D and Excoffier, L (2000) Arlequin, Version 2.0: A Software for Population Genetic Analysis. Geneva: University of Geneva, Genetics and Biometry Lab.Google Scholar
Swofford, DL (2002) PAUP*: Phylogenetic Analysis using Parsimony (*and Other Methods), Version 4.0b10. Sunderland, MA: Sinauer Associates.Google Scholar
Varshney, RK, Graner, A and Sorrells, M (2005) Genetic microsatellite markers in plants: features and applications. Trends in Biotechnology 23: 48–55.Google Scholar
Wang, RR-C (1992) Genome relationships in the perennial Triticeae based on diploid hybrids and beyond. Hereditas 116: 133–136.CrossRefGoogle Scholar
Wang, RR-C and Jensen, KB (2009) Chapter 3: wheatgrasses and wildryes. In: Singh, RJ (ed.) Genetic Resources, Chromosome Engineering, and Crop Improvement. Vol. 5 Forage Crops. Boca Raton, FL: CRC Press, pp. 41–79.Google Scholar
Weir, B (1990) Genetic Data Analysis: Methods for Desecrate Population Genetic Data. Sunderland, MA: Sinauer Associates.Google Scholar
Zhang, HB and Dvorak, J (1991) The genome origin of tetraploid species of Leymus (Poaceae: Triticeae) inferred from variation in repeated nucleotide sequences. American Journal of Botany 78: 871–884.CrossRefGoogle Scholar
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