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Prospection of ISSR primers and population genetic characterization of Paratecoma peroba (Record) Kuhlm.

Published online by Cambridge University Press:  04 September 2023

Tábatta Caroline Cerri França Open the ORCID record for Tábatta Caroline Cerri França [Opens in a new window] ,
Adelson Lemes da Silva Júnior Open the ORCID record for Adelson Lemes da Silva Júnior [Opens in a new window] ,
Leticia Rigo Tavares Open the ORCID record for Leticia Rigo Tavares [Opens in a new window] ,
Lucimara Cruz de Souza Open the ORCID record for Lucimara Cruz de Souza [Opens in a new window] ,
Fábio Demolinari de Miranda Open the ORCID record for Fábio Demolinari de Miranda [Opens in a new window]  and
Marcos Vinicius Winckler Caldeira Open the ORCID record for Marcos Vinicius Winckler Caldeira [Opens in a new window]
Tábatta Caroline Cerri França
Affiliation:
Universidade Federal do Espírito Santo, Alegre, Espírito Santo, Brazil
Adelson Lemes da Silva Júnior*
Affiliation:
Universidade Federal de Lavras, Lavras, Minas Gerais, Brazil
Leticia Rigo Tavares
Affiliation:
Universidade Federal do Espírito Santo, Alegre, Espírito Santo, Brazil
Lucimara Cruz de Souza
Affiliation:
Universidade Federal de Lavras, Lavras, Minas Gerais, Brazil
Fábio Demolinari de Miranda
Affiliation:
Universidade Federal do Espírito Santo, Alegre, Espírito Santo, Brazil
Marcos Vinicius Winckler Caldeira
Affiliation:
Universidade Federal do Espírito Santo, Jerônimo Monteiro, Espírito Santo, Brazil
*
Corresponding author: Adelson Lemes da Silva Júnior; Email: adelsonlemes@yahoo.com.br
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Abstract

The objective of this study is to prospect ISSR primers (Inter Simple Sequence Repeats) and, further genetic characterization in Paratecoma peroba. For this, leaf samples of 20 individuals were collected in a forest fragment, located in a private area, close to the Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo, in the city of Alegre, ES, Brazil. For this, 43 ISSR primers were tested, and 10 primers were selected, which provided a total of 91 bands, with 57 polymorphic bands, resulting in 62.63% polymorphism. The polymorphic information content (PIC = 0.27) indicated moderate informativeness of the primers and, therefore, they are efficient in studies with the species. However, the values found for genetic parameters such as the number of observed (AO = 1.68) and effective (AE = 1.41) alleles and, the genetic diversity indices of Nei (H* = 0.23) and Shannon (I* = 0.35) indicate the occurrence of homozygous loci and low genetic diversity in the population. On the other hand, the genetic structure evaluated by the Bayesian approach revealed the formation of three genetic groups distributed in all sampled individuals, inferring once again about the occurrence of loci in homozygosity. Therefore, the connection of neighbouring fragments and the establishment of individuals obtained from other sources could increase the genetic diversity of the population and reduce the possible effects of depression by inbreeding and genetic drift.

Keywords

Bignoniaceaeforest improvementgenetic conservationperoba-do-campo
Type
Research Article
Information
Plant Genetic Resources , Volume 21 , Issue 3 , June 2023 , pp. 211 - 217
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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Footnotes

†

In memory.

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