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Polymorphism and resistance spectrum to Magnaporthe oryzae analysis of Pi-d2 haplotypes in rice (Oryza sativa L.) resource from Yunnan province of China

Published online by Cambridge University Press:  22 May 2024

Yi Yang Open the ORCID record for Yi Yang [Opens in a new window] ,
Jinbin Li ,
Jiqiong Ma Open the ORCID record for Jiqiong Ma [Opens in a new window] ,
Jing Tao ,
Chengpeng Li ,
Yiding Sun  and
Minghui Xu Open the ORCID record for Minghui Xu [Opens in a new window]
Yi Yang
Affiliation:
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences /Yunnan Provincial Key Lab of Agricultural Biotechnology/Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture /Scientific Observation Station for Rice Germplasm Resources of Yunnan, Ministry of Agriculture, Kunming, Yunnan Province, China
Jinbin Li
Affiliation:
Agricultural Environment and Resources Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan Province, China
Jiqiong Ma
Affiliation:
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences /Yunnan Provincial Key Lab of Agricultural Biotechnology/Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture /Scientific Observation Station for Rice Germplasm Resources of Yunnan, Ministry of Agriculture, Kunming, Yunnan Province, China
Jing Tao
Affiliation:
Engineering Research Center of Vegetable Germplasm Innovation and Support Production Technology, Horticultural Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan Province, China
Chengpeng Li
Affiliation:
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences /Yunnan Provincial Key Lab of Agricultural Biotechnology/Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture /Scientific Observation Station for Rice Germplasm Resources of Yunnan, Ministry of Agriculture, Kunming, Yunnan Province, China
Yiding Sun*
Affiliation:
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences /Yunnan Provincial Key Lab of Agricultural Biotechnology/Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture /Scientific Observation Station for Rice Germplasm Resources of Yunnan, Ministry of Agriculture, Kunming, Yunnan Province, China
Minghui Xu*
Affiliation:
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences /Yunnan Provincial Key Lab of Agricultural Biotechnology/Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture /Scientific Observation Station for Rice Germplasm Resources of Yunnan, Ministry of Agriculture, Kunming, Yunnan Province, China
*
Corresponding author: Yiding Sun; Email: yidingsun18@163.com; Minghui Xu; Email: ynxuminghui@163.com
Corresponding author: Yiding Sun; Email: yidingsun18@163.com; Minghui Xu; Email: ynxuminghui@163.com
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Abstract

Pi-d2, which encodes a potential serine-threonine receptor-like kinase (RLK) membrane-spanning protein consisting of 825 amino acids, confers resistance to Magnaporthe oryzae strain ZB15 via an unidentified recognition mechanism. In this study, the Pid2 alleles of 303 rice (O. sativa) varieties from China's Yunnan region were amplified and sequenced in order to produce 24 haplotypes and 16 translation variants. Six of twenty-four alleles possessing the resistant site at the 441st amino acid were chosen for evaluating blast resistance by transforming into the blast-vulnerable rice variety Nipponbare. After being infected with 11 strains of M. oryzae, all transgenic lines exhibited resistance to ZB-15, whereas resistance to other strains varied. Notably, Pi-d2_H23 and Pi-d2_H24 exhibited resistance to all M. oryzae strains tested, indicating that these two alleles may have a broader resistance spectrum to M. oryzae. Alignment of these alleles’ amino acid sequences revealed that the differences in blast resistance spectra were primarily related to the amino acids present in the PAN domain at position 363 (valine/alanine). These findings suggested that the two extracellular signal recognition domains of PI-D2, B-lectin and PAN, may play a role in the identification of M. oryzae effectors. The present results provide insight into the mechanism of interaction between RLKs and M. oryzae.

Keywords

blast diseasebroad-spectrum resistancePi-d2
Type
Research Article
Information
Plant Genetic Resources , First View , pp. 1 - 9
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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Footnotes

Key message: Multiple Pi-d2 haplotypes were found in rice (O. sativa) resources of Yunnan. B-lectin domain and PAN domain of PI-D2 may be co-involved in the identification of effector of M. oryzae.

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