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Physiological and transcriptional status of genetically diverse perennial ryegrass (Lolium perenne L.) populations under drought stress

Published online by Cambridge University Press:  06 November 2023

Gürkan Demirkol Open the ORCID record for Gürkan Demirkol [Opens in a new window] ,
Anıl Fırat Felek Open the ORCID record for Anıl Fırat Felek [Opens in a new window] ,
Özlem Önal Aşcı Open the ORCID record for Özlem Önal Aşcı [Opens in a new window]  and
Nuri Yılmaz Open the ORCID record for Nuri Yılmaz [Opens in a new window]
Gürkan Demirkol*
Affiliation:
Department of Field Crops, Faculty of Agriculture, Tokat Gaziosmanpaşa University, Tokat, Türkiye
Anıl Fırat Felek
Affiliation:
Department of Plant Protection, Faculty of Agriculture, Ordu University, Ordu, Türkiye
Özlem Önal Aşcı
Affiliation:
Department of Field Crops, Faculty of Agriculture, Ordu University, Ordu, Türkiye
Nuri Yılmaz
Affiliation:
Department of Field Crops, Faculty of Agriculture, Ordu University, Ordu, Türkiye
*
Corresponding author: Gürkan Demirkol; Email: gurkan.demirkol@gop.edu.tr
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Abstract

This study was designed to understand how drought stress impact on the physiological and molecular status of genetically diverse Lolium perenne L. (perennial ryegrass) populations. The sensitivities of the 36 perennial ryegrass populations were checked against PEG-6000-simulated drought stress by analysing physiological status. Afterward, drought-related gene expressions were evaluated under normal and stressed conditions. The drought stress significantly up-regulated the drought-related genes in P6, P10, P15 and P30, while no difference was observed in drought-sensitive ones. The results collectively showed that four populations (P6, P10, P15, P30) are tolerant to drought stress and activate drought-related genes. Genetic similarities of the populations were also monitored using microsatellite (SSR) markers. The ten SSR primers detected 38 alleles. Surprisingly, the drought-tolerant populations were classified into different groups by principal component (PCA) and cluster analysis, showing that they are genetically different. Moreover, four unique alleles were identified by two SSR primers (P-07, and P-08) in P6, P10 and P15. In addition, the drought-tolerant populations (P6, P10, P15, P30) can be used to develop new perennial ryegrass cultivars for areas having drought problems.

Keywords

abiotic stressallelic variationdroughtpolymorphism
Type
Research Article
Information
Plant Genetic Resources , Volume 21 , Issue 4 , August 2023 , pp. 331 - 339
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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