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Nuclear SSR-based genetic diversity and STRUCTURE analysis of Greek tomato landraces and the Greek Tomato Database (GTD)

Published online by Cambridge University Press:  28 February 2024

Androniki C. Bibi Open the ORCID record for Androniki C. Bibi [Opens in a new window] ,
John Marountas ,
Konstantina Katsarou ,
Anastasios Kollias ,
Pavlos Pavlidis ,
Eleni Goumenaki Open the ORCID record for Eleni Goumenaki [Opens in a new window]  and
Dimitris Kafetzopoulos
Androniki C. Bibi*
Affiliation:
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece Department of Biology, University of Crete, Heraklion, Greece
John Marountas
Affiliation:
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece
Konstantina Katsarou
Affiliation:
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece Department of Biology, University of Crete, Heraklion, Greece
Anastasios Kollias
Affiliation:
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece
Pavlos Pavlidis
Affiliation:
Department of Biology, University of Crete, Heraklion, Greece Institute of Computer Science (ICS), Foundation for Research and Technology - Hellas (FORTH), Heraklion, Greece
Eleni Goumenaki
Affiliation:
Department of Agriculture, Hellenic Mediterranean University, P.O. Box 1939, GR 71004, Heraklion, Crete, Greece
Dimitris Kafetzopoulos
Affiliation:
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece
*
Corresponding author: Androniki C. Bibi; Email: androniki_bibi@imbb.forth.gr
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Abstract

Tomato has been cultivated in Greece for more than 200 years, even though is not native to the country. Greece with a favourable environment all-round the year, has become a major competitor in tomato production around Europe. However, there is an increasing demand to improve tomato crop, to withstand harsh environmental conditions (extreme temperatures, salinity, etc.), and to develop high-quality final products. People have devoted a significant effort to crop improvement through phenotypic screening resulting in a large number of tomato landraces. An increasing demand to clarify the relationships among local tomato landraces and hybrids utilizing the most preferred molecular markers the simple sequence repeats (SSR-markers) is the main objective of this study. Twenty-seven tomato landraces and two tomato hybrids cultivated in Crete, Greece, were genotyped utilizing eleven simple sequence repeats (SSR) along with the Structure analysis of the germplasm. A neighbour-joining dendrogram of the 27 landraces and the two hybrids was produced. The Structure analysis indicated that nine ancestral populations are hidden inside the group of all the genotypes tested, using Evanno's method. The final objective was to make these data publicly available through the first Greek relational database (Greek Tomato Database-GTD). GTD was developed allowing the users to update and enrich the database, with new and supplemental information. This work is the first molecular fingerprint of the 27 landraces of Greece which is documented along with the phenotypic information in the GTD.

Keywords

Greek Tomato DatabaseMDS analysisSSR markersStructure analysistomato-landraces
Type
Research Article
Information
Plant Genetic Resources , Volume 22 , Issue 2 , April 2024 , pp. 107 - 116
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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Footnotes

†

Diseased.

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