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Molecular characterization of almond accessions from the island of La Palma (Canary Islands, Spain) using SSR markers

Published online by Cambridge University Press:  27 February 2014

Guillermo Padilla ,
Rafel Socias i Company  and
Amando Ordás
Guillermo Padilla*
Affiliation:
Instituto de Productos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Astrofísico Francisco Sánchez 3, 38206 La Laguna, Santa Cruz de Tenerife, Spain
Rafel Socias i Company
Affiliation:
Unidad de Fruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Av. Montañana 930, 50059Zaragoza, Spain
Amando Ordás
Affiliation:
Misión Biológica de Galicia, Consejo Superior de Investigaciones Científicas (CSIC), Apdo. 28, 36080Pontevedra, Spain
*
* Corresponding author. E-mail: gpadilla@cib.csic.es
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Abstract

In this study, 15 simple sequence repeat (SSR) markers were used for genetic diversity analysis of 45 almond accessions, which included 25 local cultivars from La Palma Island and three other commercial cultivars. A total of 110 amplification fragments were produced, with an average value of 7.9 alleles per locus. Twelve of the SSR markers can be considered as highly informative, with values of expected heterozygosity and power of discrimination above 0.5 and 0.8, respectively. Due to cases of synonymy and homonymy, 37 different genetic profiles were obtained, with the homonymy of the soft-shell varieties known as ‘Mollar’ being the most significant. Cluster analysis identified four groups within the accessions. One of these groups exclusively consisted of the two commercial cultivars ‘Guara’ and ‘Ferraduel’. The other commercial cultivar used in the study, ‘Desmayo Largueta’, was in a cluster with three cultivars from the same locality. The analysis of molecular variance revealed that the within-localities component accounts for most of the total variation, suggesting that La Palma almond cultivars did not originate independently in different parts of the island. The results of the study reveal the genetic singularity of La Palma almond cultivars and the genetic diversity among them.

Keywords

genetic diversityhomonymylocal cultivarsmicrosatellitesPrunus dulcissynonymy
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue 3 , December 2014 , pp. 323 - 329
Copyright
Copyright © NIAB 2014 

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