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Genetic diversity in Vicia faba L. populations cultivated in Tunisia revealed by simple sequence repeat analysis

Published online by Cambridge University Press:  06 February 2014

Yassine Yahia ,
Hédia Hannachi ,
Antonio Jose Monforte ,
James Cockram ,
Mohamed Loumerem ,
Belkacem Zarouri  and
Ali Ferchichi
Yassine Yahia*
Affiliation:
Arid and Oases Cropping Laboratory, Arid Lands Institute (IRA), Médenine 4119, Tunisia
Hédia Hannachi
Affiliation:
Arid and Oases Cropping Laboratory, Arid Lands Institute (IRA), Médenine 4119, Tunisia
Antonio Jose Monforte
Affiliation:
Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia (UPV)-Consejo Superior de Investigaciones Científicas (CSIC), Ciudad Politécnica de la Innovación (CPI), Ed. 8E, Ingeniero Fausto Elio s/n, 46022Valencia, Spain
James Cockram
Affiliation:
National Institute of Agricultural Botany (NIAB), Huntington Road, CambridgeCB3 0LE, UK
Mohamed Loumerem
Affiliation:
Arid and Oases Cropping Laboratory, Arid Lands Institute (IRA), Médenine 4119, Tunisia
Belkacem Zarouri
Affiliation:
Departamento de Investigación Agroalimentaria, Instituto Madrileño de Investigación y Desarrollo, IMIDRA, Madrid, Spain
Ali Ferchichi
Affiliation:
Arid and Oases Cropping Laboratory, Arid Lands Institute (IRA), Médenine 4119, Tunisia
*
* Corresponding author. E-mail: yahia.yassine@gmail.com
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Abstract

Faba bean (Vicia faba L.) is one of the most important legumes in the world. Little is known about the genetic resources of faba bean in Southern Tunisia. In the present study, genetic diversity within Tunisian faba bean germplasms was investigated using 16 simple sequence repeat markers. In total, 50 alleles were detected. The number of alleles per marker ranged from 2 to 6, with an average of 3. Genetic diversity and polymorphism information content values averaged, respectively, 0.43 (range 0.34–0.51) and 0.36 (range 0.28–0.43). The mean heterozygosity value was 0.27. A model-based structure analysis based on neighbour-joining tree and factorial correspondence analysis revealed the presence of two subpopulations, consistent with the clustering based on genetic distance (GD). The overall Fis value was 0.36, indicating the importance of selfing in these populations. Analysis of molecular variance revealed that the within-population genetic variance component was much higher than the between-population or between-subpopulation variance component. The genetic relationships based on Nei's GD revealed that AGD (Aguadulce) and SAG (Super Aguadulce) and TF1 and TF2 (Tafartassa-Gafsa) were the most closely related populations. Assessment of genetic variation within faba bean populations will be informative for the conservation of germplasms and the implementation of effective breeding programmes in Tunisia.

Keywords

genetic diversityplant genetic resource conservationpopulation structuresimple sequence repeats (SSRs)Vicia faba L.
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue 3 , December 2014 , pp. 278 - 285
Copyright
Copyright © NIAB 2014 

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