Hostname: page-component-8448b6f56d-t5pn6 Total loading time: 0 Render date: 2024-04-20T04:41:30.663Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic diversity in the USDA Limnanthes germplasm collection assessed by simple sequence repeats

Published online by Cambridge University Press:  01 April 2009

Linda M. Donnelly ,
Maria M. Jenderek ,
James P. Prince ,
Patrick A. Reeves ,
Allan Brown  and
Richard M. Hannan
Linda M. Donnelly
Affiliation:
National Arid Land Plant Genetic Research Unit, USDA-ARS, Parlier, CA 93648, USA Department of Biology, California State University Fresno, Fresno, CA 93740, USA
Maria M. Jenderek*
Affiliation:
National Arid Land Plant Genetic Research Unit, USDA-ARS, Parlier, CA 93648, USA
James P. Prince
Affiliation:
Department of Biology, California State University Fresno, Fresno, CA 93740, USA
Patrick A. Reeves
Affiliation:
National Center for Genetic Resources Preservation, USDA-ARS, Fort Collins, CO 80521, USA
Allan Brown
Affiliation:
National Arid Land Plant Genetic Research Unit, USDA-ARS, Parlier, CA 93648, USA
Richard M. Hannan
Affiliation:
Western Regional Plant Introduction Station, USDA-ARS, Pullman, WA 99164, USA
*
*Corresponding author. E-mail: maria.jenderek@ars.usda.gov
Get access Rights & Permissions [Opens in a new window]

Abstract

The genus Limnanthes (Limnanthaceae), also known as meadowfoam, has attracted attention for industrial use due to the unique characteristics of its seed oil. Samples from wild populations showed variability in agronomically important traits involved in seed oil yield, warranting the establishment and continued development of a germplasm collection. The level of genetic diversity within the United States Department of Agriculture, Agriculture Research Service, National Plant Germplasm System (USDA-ARS-NPGS) Limnanthes collection was evaluated using 15 simple sequence repeat (SSR) markers across 62 accessions representing 7 species. Parsimony analysis separated the accessions into two main groups consistent with the traditional taxonomic sections Inflexae and Reflexae, but there was little resolution within groups. These two groups were confirmed using neighbour-joining analysis and principal coordinate analysis. SSR marker variation suggests that the Limnanthes germplasm collection is genetically diverse and the accessions within the species likely contain novel alleles, and therefore the collection contributes to the conservation of the wild Limnanthes gene pool.

Keywords

conservation geneticsgenetic diversitygermplasm resourcesmeadowfoamsimple sequence repeat
Type
Research Article
Information
Plant Genetic Resources , Volume 7 , Issue 1 , April 2009 , pp. 33 - 41
Copyright
Copyright © NIAB 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bagby, MO, Smith, CR, Miwa, TK, Lohmar, RL and Wolff, IA (1961) A unique fatty acid from Limnanthes douglasii seed oil: the C22 diene. Journal of Organic Chemistry 26: 1261–1265.CrossRefGoogle Scholar
Baranger, A, Aubert, G, Arnau, G, Laine, AL, Denoit, G, Potier, J, Weinachter, C, Lejeune-Henaut, I, Lallemand, J and Burstin, J (2004) Genetic diversity within Pisum sativum using protein- and PCR-based markers. Theoretical and Applied Genetics 108: 1309–1321.CrossRefGoogle ScholarPubMed
California Native Plant Society (CNPS) (2005) Inventory of rare and endangered plants [online edition v6-05c]. Sacramento, CA: California Native Plant Society, http://www.cnps.org/inventory.Google Scholar
Charters, YM, Robertson, A and Wilkinson, MJ (1996) PCR analysis of oilseed rape cultivars (Brassica napus L. ssp. oleifera) using 5′-anchored simple sequence repeat (SSR) primers. Theoretical and Applied Genetics 92: 442–447.CrossRefGoogle ScholarPubMed
Cordeiro, GM, Taylor, GO and Henry, RJ (2000) Characterization of microsatellite markers from sugarcane (Saccharum sp.), a highly polyploidy species. Plant Science 155: 161–168.CrossRefGoogle Scholar
Debry, RW and Olmstead, RG (2000) A simulation study of reduced tree-search effort in bootstrap resampling analysis. Systematic Biology 49: 171–179.Google ScholarPubMed
Dillon, SL, Lawrence, PK and Henry, RJ (2005) The new use of Sorghum bicolor-derived SSR markers to evaluate genetic diversity in 17 Australian Sorghum species. Plant Genetic Resources 3(1): 19–28.CrossRefGoogle Scholar
Dole, JA and Sun, M (1992) Field and genetic survey of the endangered Butte County meadowfoam – Limnanthes floccosa ssp. californica (Limnanthaceae). Conservation Biology 6(4): 549–558.CrossRefGoogle Scholar
Gentry, HS and Miller, RW (1965) The search for new industrial crops IV. Prospectus of Limnanthes. Economic Botany 19(1): 25–32.CrossRefGoogle Scholar
Gilbert, JE, Lewis, RV, Wilkinson, MJ and Caligari, PDS (1999) Developing an appropriate strategy to assess genetic variability in plant germplasm collections. Theoretical and Applied Genetics 98: 1125–1131.CrossRefGoogle Scholar
Godwin, ID, Mace, E and Nurzuhairawaty, (2001) Genotyping Pacific Island taro (Colocasia esculenta (L.) Schott) germplasm. In: Henry, RJ (ed.) Plant Genotyping: The DNA Fingerprinting of Plants. New York: NY: CAB International, pp. 109–128.CrossRefGoogle Scholar
Havey, MJ (1995) Identification of cytoplasms using the polymerase chain reaction to aid in the extraction of maintainer lines from open-pollinated populations of onion. Theoretical and Applied Genetics 90: 263–268.CrossRefGoogle ScholarPubMed
Higgins, JJ, Calhoun, W, Willingham, BC, Dinkel, DH, Raisler, WL and White, GA (1971) Agronomic evaluation of prospective new crop species II. The American Limnanthes. Economic Botany 25: 44–54.CrossRefGoogle Scholar
Jain, SK and Abuelgasim, EH (1981) Some yield components and ideotype traits in meadowfoam, a new industrial crop. Euphytica 30: 437–443.CrossRefGoogle Scholar
Jain, SK, Pierce, RO and Hauptli, H (1977) Meadowfoam: potential new oil crop. California Agriculture 31: 18–20.Google Scholar
Jolliff, GD, Tinsley, IJ, Calhoun, W and Crane, JM (1981) Meadowfoam (Limnanthes alba) Its research and development as a potential new oilseed crop for the Willamette Valley of Oregon. Corvallis, Agriculture Experiment Station, Oregon State University, Corvallis Station Bulletin 648: November 1981: 17.Google Scholar
Kishore, VK, Velasco, P, Shintani, DK, Rowe, J, Rosato, C, Adair, N, Slabaugh, MB and Knapp, SJ (2004) Conserved simple sequence repeats in the Limnanthaceae (Brassicales). Theoretical and Applied Genetics 108(3): 450–457.CrossRefGoogle ScholarPubMed
Lodhi, MA, Ye, GN, Weeden, NF and Reisch, BI (1994) A simple and efficient method for DNA extraction from grapevine cultivars, Vitis species and Amplelopsis. Plant Molecular Biology Reporter 12(1): 6–13.CrossRefGoogle Scholar
Mason, CT Jr (1952) A systematic study of the genus Limnanthes. University of California Publication in Botany 25: 455–512.Google Scholar
Matsuoka, Y, Mitchell, SE, Kresovich, S, Goodman, M and Doebley, J (2002) Microsatellites in Zea – variability, patterns of mutations, and use for evolutionary studies. Theoretical and Applied Genetics 104: 436–450.CrossRefGoogle ScholarPubMed
McLauchlan, A, Henry, RJ, Isaac, PG and Edwards, KJ (2001) Microsatellite analysis in cultivated hexaploid wheat and wild wheat relatives. Plant Genotyping: The DNA Fingerprinting of Plants. New York: NY: CAB International, pp. 147–159.CrossRefGoogle Scholar
Miwa, TK and Wolff, IA (1962) Fatty acids, fatty alcohols, and wax esters from Limnanthes douglasii (Meadowfoam) seed oil. Journal of the American Oil Chemists' Society 39: 320–322.CrossRefGoogle Scholar
Panaud, O, Chen, X and McCouch, SR (1996) Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Molecular Genetics and Geneticist 252: 597–607.Google ScholarPubMed
Pierce, RO and Jain, SK (1977) Variation in some plant and seed oil characteristics of meadowfoam. Crop Science 17: 521–526.CrossRefGoogle Scholar
Reeves, PA and Richards, CM (2007) Distinguishing terminal monophyletic groups from reticulate taxa: performance of phenetic, tree-based, and network procedures. Systematic Biology 56(2): 302–320.Google Scholar
Sham, P, Bader, JS, Craig, I, O'Donovan, M and Owen, M (2002) DNA pooling: a tool for large-scale association studies. Nature Reviews Genetics 3: 862–871.CrossRefGoogle ScholarPubMed
Smith, CR, Bagby, MO, Miwa, TK, Lohmar, RL and Wolff, IA (1960) Unique fatty acids from Limnanthes douglasii seed oil: the C20- and C22-monoenes. Journal of Organic Chemistry 25: 1770–1774.CrossRefGoogle Scholar
Swofford, DL (2002) PAUP*: Phylogenetic Analysis using Parsimony and Other Methods (software). Sunderland, MA: Sinauer Associates.Google Scholar
USDA, ARS National Plant Germplasm System. Germplasm Resources Information Network (GRIN) [online database]. http://www.ars-grin.gov/cgi-bin/npgs/html.Google Scholar
Wiens, JJ (2006) Missing data and the design of phylogenetic analyses. Journal of Biomedical Informatics 39: 34–42.CrossRefGoogle ScholarPubMed