Skip to main content Accessibility help
×
Home

Development of chloroplast microsatellite markers for identification of Glycyrrhiza species

  • Kyung Jun Lee (a1), Sebastin Raveendar (a1), Ji Seon Choi (a2), Jinsu Gil (a2), Jeong Hoon Lee (a3), Yoon-Sup So (a4) and Jong-Wook Chung (a2)...

Abstract

Licorice (Glycyrrhiza glabra) is an important medicinal herb and has long been used in traditional medicine for the treatment of several diseases worldwide. Understanding the genetic diversity within Glycyrrhiza species is important for the efficient conservation of these medicinal herbs. In this study, we have developed 20 polymorphic chloroplast microsatellite (cpSSR) markers using the chloroplast genome of G. lepidota. The cpSSR markers were tested on a total of 27 Glycyrrhiza individual plants. The number of alleles per locus ranged from two to eight among the Glycyrrhiza accessions. Overall, the Shannon index (I) for each cpSSR ranged from 0.315 to 1.694, the diversity indices (h) were 0.140–0.793 and the unbiased diversity indices (uh) were 0.145–0.825. In addition, the cpSSR markers were successfully divided and classified the 27 Glycyrrhiza individuals into four groups. The cpSSR markers developed in this study could be used in the assessment of genetic diversity and rapid identification of Glycyrrhiza species.

Copyright

Corresponding author

*Corresponding author. E-mail: yoonsupso@chungbuk.ac.kr and jwchung73@chungbuk.ac.kr

Footnotes

Hide All

These authors contributed equally to this work.

Footnotes

References

Hide All
Ashurmetov, OA (1996) Selection of parental pairs for obtaining hybrids in the genera Glycyrrhiza L. and Meristotropis Fisch. et Mey. Genetic Resources and Crop Evolution 43: 167171.
Cheng, Y, de Vicente, MC, Meng, H, Guo, W, Tao, N and Deng, X (2005) A set of primers for analyzing chloroplast DNA diversity in Citrus and related genera. Tree Physiology 25: 661672.
Diekmann, K, Hodkinson, TR and Barth, S (2012) New chloroplast microsatellite markers suitable for assessing genetic diversity of Lolium perenne and other related grass species. Annals of Botany 110: 13271339.
Fiore, C, Eisenhut, M, Krausse, R, Ragazzi, E, Pellati, D, Armanini, D and Bielenberg, J (2008) Antiviral effects of Glycyrrhiza species. Phytotherapy Research 22: 141148.
Green, RM, Vardi, A and Galun, E (1986) The plastome of Citrus. Physical map, variation among Citrus cultivars and species and comparison with related genera. Theoretical and Applied Genetics 72: 170177.
Jung, J, Kim, KH, Yang, K, Bang, K-H and Yang, T-J (2014) Practical application of DNA markers for high-throughput authentication of Panax ginseng and Panax quinquefolius from commercial ginseng products. Journal of Ginseng Research 38: 123129.
Liu, K and Muse, SV (2005) Powermarker: an integrated analysis environment for genetic marker analysis. Bioinformatics (Oxford, England) 21: 21282129.
Peakall, R and Smouse, PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Resources 6: 288295.
Powell, W, Morgante, M, McDevitt, R, Vendramin, GG and Rafalski, JA (1995) Polymorphic simple sequence repeat regions in chloroplast genomes: applications to the population genetics of pines. Proceedings of the National Academy of Sciences of the USA 92: 77597763.
Raveendra, KR, Jayachandra, , Srinivasa, V, Sushma, KR, Allan, JJ, Goudar, KS, Shivaprasad, HN, Venkateshwarlu, K, Geetharani, P, Sushma, G and Agarwal, A (2012) An extract of Glycyrrhiza glabra (GutGard) alleviates symptoms of functional dyspepsia: a randomized, double-blind, placebo-controlled study. Evidence-Based Complementary and Alternative Medicine 2012: 9.
Raveendar, S, So, Y-S, Lee, KJ, Lee, D-J, Sung, J and Chung, J-W (2017) The complete chloroplast genome sequence of Glycyrrhiza lepidota (Nutt.) Pursh – an American wild licorice. Journal of Crop Science and Biotechnology 20: 295303.
Tamura, K, Dudley, J, Nei, M and Kumar, S (2007) MEGA4: molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Molecular Biology and Evolution 24: 15961599.

Keywords

Type Description Title
WORD
Supplementary materials

Lee et al. supplementary material
Lee et al. supplementary material 1

 Word (44 KB)
44 KB

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed