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Assessment of salt tolerance and analysis of the salt tolerance gene Ncl in Indonesian soybean germplasm

  • D. Cao (a1) (a2), Y. L. Yan (a1) (a3) and D. H. Xu (a1)


Soybean [Glycine max (L.) Merr.] is one of the most important legume crops in the world. However, soybean grain yield is extensively affected by environmental stresses such as soil salinity. In this study, we evaluated the germplasm of 51 Indonesian soybean accessions for salt tolerance to identify salt-tolerant germplasms for possible use in breeding for soybean salt tolerance. Based on experiments under hydroponic conditions, adding 100 mM of NaCl to a 1/2 concentration of Hoagland and Arnon solution, several Indonesian soybean germplasms, such as Java 7, Seputih Raman, Tambora, Ringgit (JP 30217), Sinyonya (early) and Sinyonya (late) were identified as salt-tolerant in terms of salt tolerance rate (STR) and leaf chlorophyll content (SPAD value) taken with the Konica Minolta SPAD-502 chlorophyll meter. The selected salt-tolerant germplasms were further evaluated under soil medium cultivation in pots irrigated with 100 mM NaCl for around 5 weeks. The six selected soybean germplasms again showed higher salt tolerance in terms of SPAD, STR and shoot dry weight. Expression analysis of the salt tolerance gene Ncl revealed a significant positive correlation between Ncl expression and salt tolerance, suggesting that Ncl is essential for salt tolerance in the Indonesian soybean germplasms we tested. The salt-tolerant Indonesian soybean germplasms identified in this study could be used in local soybean breeding practices for the improvement of salt tolerance.


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Abel, GH (1969) Inheritance of the capacity for chloride inclusion and chloride exclusion by soybeans. Crop Science 9: 697698.
Abel, GH and MacKenzie, AJ (1964) Salt tolerance of soybean varieties (Glycine max L. Merill) during germination and later growth. Crop Science 4: 157161.
Chang, RZ, Chen, YW, Shao, GH and Wan, CW (1994) Effect of salt stress on agronomic characters and chemical quality of seeds in soybean. Soybean Science 13: 101105 (in Chinese with English abstract).
Chen, H.Cui, S, Fu, S, Gai, J and Yu, D (2008) Identification of quantitative trait loci associated with salt tolerance during seedling growth in soybean (Glycine max L.). Australian Journal of Agricultural Research 59: 10861091.
Do, TD, Chen, H, Vu, HTT, Hamwieh, A, Yamada, T, Sato, T, Yan, YL, Cong, H, Shono, M, Suenaga, K and Xu, D (2016) Ncl synchronously regulates Na+, K+, and Cl in soybean and greatly increases the grain yield in saline field conditions. Scientific Reports 6: 19147.
Guan, R, Qu, Y, Guo, Y, Yu, L, Liu, Y, Jiang, J, Chen, J, Ren, Y, Liu, G, Tian, L, Jin, L, Liu, Z, Hong, H, Chang, R, Gilliham, M and Qiu, L (2014) Salinity tolerance in soybean is modulated by natural variation in GmSALT3. Plant Journal 80: 937950.
Ha, BK, Vuong, TD, Velusamy, V, Nguyen, HT, Shannon, JG and Lee, JD (2013) Genetic mapping of quantitative trait loci conditioning salt tolerance in wild soybean (Glycine soja) PI 483463. Euphytica 193: 7988.
Hamwieh, A and Xu, DH (2008) Conserved salt tolerance quantitative trait locus (QTL) in wild and cultivated soybeans. Breed Science 58: 355359.
Hamwieh, A, Tuyen, H, Cong, ER, Benitez, R, Takahashi, and Xu, DH (2011) Identification and validation of a major QTL for salt tolerance in soybean. Euphytica 170: 451459.
Hymowitz, T (2004) Speciation and cytogenetics. In Boerma, HR and Specht, JE (eds) Soybeans: Improvement, Production and Uses, 3rd edn. Madison: American Society of Agronomy, pp. 97136.
Lee, GJ, Boerma, HR, Villagarcia, MR, Zhou, X, Carter, J TE, Li, Z and Gibbs, MO (2004) A major QTL conditioning salt tolerance in S-100 soybean and descendent cultivars. Theoretical and Applied Genetics 109: 16101619.
Livak, KJ and Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods 25: 402408.
Munns, R and Tester, M (2008) Mechanisms of salinity tolerance. Annual Review of Plant Biology 59: 651681.
Parker, MB, Gascho, GJ and Gains, TP (1983) Chloride toxicity of soybeans grown on Atlantic Coast flatwoods soils. Agronomy Journal 75: 439443.
Phang, TH, Shao, G and Lam, HM (2008) Salt tolerance in soybean. Journal of Integrative Plant Biology 50: 11961212.
Qi, X, Li, M, Xie, M, Liu, X, Ni, M, Shao, G, Song, C, Yim, AKY, Tao, Y, Wong, F, Isobe, S, Wong, C, Wong, K, Xu, C, Li, C, Wang, Y, Guan, R, Sun, F, Fan, G, Xiao, Z, Zhou, F, Phang, T, Liu, X, Tong, S, Chan, T, Yiu, S, Tabata, S, Wang, J, Xu, X and Lam, H (2014) Identification of a novel salt tolerance gene in wild soybean by whole-genome sequencing. Nature Communications 5: 4340.
Shao, GH, Song, JZ and Liu, HL (1986) Preliminary studies on the evaluation of salt tolerance in soybean varieties. Acta Agronomic Sinica 6: 3035 (in Chinese with English abstract).
Singleton, PW and Bohlool, BB (1984) Effect of salinity on nodule formation by soybean. Plant Physiology 74: 7276.
Tuyen, DD, Lal, SK and Xu, DH (2010) Identification of a major QTL allele from wild soybean (Glycine soja Sieb. & Zucc.) for increasing alkaline salt tolerance in soybean. Theoretical and Applied Genetics 121: 229236.
Wan, C, Shao, G, Chen, Y and Yan, S (2002). Relationship between salt tolerance and chemical quality of soybean under salt stress. Chinese Journal of Oil Crop Sciences 24: 6772 (in Chinese with English abstract).
Wang, D and Shannon, MC (1999) Emergence and seedling growth of soybean cultivars and maturity groups under salinity. Plant and Soil 214: 117124.


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