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Molecular cloning and sequence analysis of 1Dx-type HMW-GS genes from different wheat varieties

Published online by Cambridge University Press:  28 October 2022

Li-Juan Yang
Xinxiang Academy of Agricultural Sciences, Xinxiang 453003, China
Xiang-Fen Zhang
Henan Seed Management Station, Zhengzhou 450041, China
Yan-Qi Dong
Xinxiang Academy of Agricultural Sciences, Xinxiang 453003, China
Yue-Ting Zheng
College of Agronomy/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450046, China
Jin-Xia Zhang
Xinxiang Academy of Agricultural Sciences, Xinxiang 453003, China
Yi-Lian He
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621002, China
Xiu-Fang Tan
Xinxiang Academy of Agricultural Sciences, Xinxiang 453003, China
Zhi-Kai Jiang
Xinxiang Academy of Agricultural Sciences, Xinxiang 453003, China
Hua-Ping Ma*
Xinxiang Academy of Agricultural Sciences, Xinxiang 453003, China
Author for correspondence: Hua-Ping Ma, E-mail:


High-molecular-weight glutenin subunits (HMW-GS) contribute to dough elasticity and bread baking quality in wheat. In this study, wheat varieties were classified based on their HMW-GS composition into three groups: 1Dx5 (5 + 10, Gaoyou 8901, Xinmai 28, Xinmai 19, Xinmai 26 and Jinbaoyin), 1Dx2 (2 + 12, Zhoumai 24, Xinmai 9 and Yumai) and 1Dx4 (4 + 12, Aikang 58). Sequence analysis showed that 1Dx-GY8901, 1Dx-XM28, 1Dx-XM19 and 1Dx-XM26 were similar to the 1Dx5 gene and clustered on the same branch, while 1Dx-AK58, 1Dx-ZM24, 1Dx-JBY, 1Dx-YM, 1Dx-XM9 and 1Dx-JBY were more similar to the 1Dx2 gene and clustered on the same branch with 1Dx.2.2. There was a mutation of Ser to Cys at position S2, for an extra Cys in the repeat regions of 1Dx-XM19, 1Dx-XM26, 1Dx-XM28 and 1Dx-GY8901. The wheat HMW-GS genes exhibited similar percentages of α-helix, extended strand, β-turn and random coil structure, with ranges of 13.33–13.59, 4.77–5.78, 7.08–9.18 and 72.3–73.94%, respectively. Sequence conservation and the composition of HMW-GS subunits were also analysed for a series of strong gluten wheat varieties, Xinmai 9 (1, 7 + 8, 2 + 12), Xinmai 19 (1, 7 + 9, 5 + 10), Xinmai 26 (1, 7 + 8, 5 + 10) and Xinmai 28 (1, 7 + 9, 5 + 10). The results of this work should facilitate future breeding efforts and provide the theoretical basis for wheat quality improvement.

Research Article
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

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