Skip to main content Accessibility help
×
Home

Comparison of flavonoid contents and antioxidant activities of Vicia species

Published online by Cambridge University Press:  29 September 2015

Kyung Jun Lee
Affiliation:
National Agrobiodiversity Center, NAAS, RDA, Jeonju 560-500, Republic of Korea
Jung-Ro Lee
Affiliation:
National Agrobiodiversity Center, NAAS, RDA, Jeonju 560-500, Republic of Korea
Hyo-Jeong Kim
Affiliation:
National Agrobiodiversity Center, NAAS, RDA, Jeonju 560-500, Republic of Korea
Sebastin Raveendar
Affiliation:
National Agrobiodiversity Center, NAAS, RDA, Jeonju 560-500, Republic of Korea
Gi-An Lee
Affiliation:
National Agrobiodiversity Center, NAAS, RDA, Jeonju 560-500, Republic of Korea
Young-Ah Jeon
Affiliation:
National Agrobiodiversity Center, NAAS, RDA, Jeonju 560-500, Republic of Korea
Eunseong Park
Affiliation:
National Agrobiodiversity Center, NAAS, RDA, Jeonju 560-500, Republic of Korea
Kyung-Ho Ma
Affiliation:
National Agrobiodiversity Center, NAAS, RDA, Jeonju 560-500, Republic of Korea
Sok-Young Lee
Affiliation:
National Agrobiodiversity Center, NAAS, RDA, Jeonju 560-500, Republic of Korea
Jong-Wook Chung
Affiliation:
National Agrobiodiversity Center, NAAS, RDA, Jeonju 560-500, Republic of Korea
Corresponding
E-mail address:

Abstract

A total of 27 accessions from ten Vicia species were investigated for flavonoid contents, total polyphenol contents, and DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS [2,2′-azinobis(3-ethylbenzothiazoline 6-sulfonic acid)] free radical-scavenging activities. The results revealed that NAC17 (V. monantha) and NAC14 (V. hyrcanica) had the highest total flavonoid content (1.42 ± 0.09 mg/g) and total polyphenol content [124.2 ± 0.5 μg/gallic acid equivalents (GAE) mg], respectively. Among four flavonoids, naringenin was detected at high concentrations in Vicia species. The DPPH and ABTS assays showed values in the range of 57.2 (IC50) (NAC13, V. faba) to 6530.0 (IC50) (NAC24, V. sativa subsp. nigra) and 19.1 μg/Trolox mg (NAC7, V. cracca) to 253.4 μg/Trolox mg (NAC13, V. faba), respectively. Among ten Vicia species, V. monantha and V. hyrcanica had the highest flavonoid content (1.31 ± 0.09 mg/g) and total polyphenol content (116.5 ± 2.0 μg/GAE mg), respectively. The highest antioxidant activity was detected in V. faba. These results will expand the flavonoid database and provide valuable information on Vicia species for the development of functional foods or feed-additive resources.

Type
Research Article
Copyright
Copyright © NIAB 2015 

Access options

Get access to the full version of this content by using one of the access options below.

References

Ajila, CM and Prasada Rao, UJ (2008) Protection against hydrogen peroxide induced oxidative damage in rat erythrocytes by Mangifera indica L. peel extract. Food and Chemical Toxicology 46: 303309.CrossRefGoogle ScholarPubMed
Akyuz, M (2013) Nutritive value: flavonoid content and radical scavenging activity of the truffle (Terfezia boudieri Chatin). Journal of Soil Science and Plant Nutrition 13: 143151.Google Scholar
Alzueta, C, Caballero, R, Rebole, A, Trevino, J and Gill, A (2001) Crude protein fraction in common vetch fresh forage during pod filling. Journal of Animal Science 79: 24492455.CrossRefGoogle ScholarPubMed
Amarowicz, R, Troszynska, A, Barylko-Pikielna, N and Shahidi, F (2004) Polyphenolics extracts from legume seeds: correlations between total antioxidant activity, total phenolics content, tannins content and astringency. Journal of Food Lipids 11: 278286.CrossRefGoogle Scholar
Amarowicz, R, Troszynska, A and Pegg, RB (2008) Antioxidative and radical scavenging effects of phenolics from Vicia sativum . Fitoterapia 79: 121122.CrossRefGoogle ScholarPubMed
Annadurai, T, Muralidharan, AR, Joseph, T, Hsu, MJ, Thomas, PA and Geraldine, P (2012) Antihyperglycemic and antioxidant effects of a flavanone, naringenin, in streptozotocin–nicotinamide-induced experimental diabetic rats. Journal of Physiology and Biochemistry 68: 307318.CrossRefGoogle ScholarPubMed
Asen, S (1984) High pressure liquid chromatographic analysis of flavonoid chemical markers in petals from Gerbera flowers as an adjunct for cultivar and germplasm identification. Phytochemistry 23: 25232526.CrossRefGoogle Scholar
Campeol, E, Catal, S, Cremonni, R and Morelii, I (2000) Flavonoids analysis of Vicia species of Narbonensis complex: V. kalakhensis Khatt., Maxt. and Bisby and V. eristalioides Maxt. Caryologia 53: 6368.CrossRefGoogle Scholar
Campeol, E, Cioni, PL, Flamini, G, Rossi, B and Cremonini, R (2003) Flavonoids analysis of four Vicia species of Narbonensis complex in two different vegetative phases. Caryologia 56: 365371.CrossRefGoogle Scholar
Choi, JS, Park, KY, Moon, SH, Rhee, SH and Young, HS (1994) Antimutagenic effect of plant flavonoids in the Salmonella assay system. Archives of Pharmacal Research 17: 7175.CrossRefGoogle ScholarPubMed
Croteau, R, Kutchan, TM and Lewis, NG (2000) Natural products (secondary metabolites). In: Buchanan, BB, Gruissem, W and Jones, RL (eds) Biochemistry and Molecular Biology of Plants. Rockville, MD: American Society of Plants Physiologists, pp. 12501318.Google Scholar
Gee, JM and Johnson, IT (2001) Polyphenolic compounds: interactions with the gut and implications for human health. Current Medicinal Chemistry 8: 12451255.CrossRefGoogle ScholarPubMed
Harborne, JB and Turner, BL (1984) Plant Chemiosystematics. London: Academic Press.Google Scholar
Hou, WC, Lin, RD, Cheng, KT, Hung, YT, Cho, CH, Chen, CH, Hwang, SY and Lee, MH (2003) Free radical scavenging activity of Taiwanese native plants. Phytomedicine 10: 170175.CrossRefGoogle ScholarPubMed
Jun, YM, Kim, EH, Lim, JJ, Kim, SH, Kim, SH, Lin, JD, Cheoi, DS, Cheoi, YS, Yu, CY and Chung, IM (2012) Variation of phenolic compounds contents in cultivated Astragalus membranaceus . Korean Journal of Medicinal Crop Science 20: 447453.CrossRefGoogle Scholar
Koh, E, Wimalasiri, KMS, Chassy, AW and Mitchell, AE (2009) Content of ascorbic acid: quercetin, kaempferol and total phenolics in commercial broccoli. Journal of Food Composition and Analysis 22: 637643.CrossRefGoogle Scholar
Kumar, MS, Unnikrishnan, MK, Patra, S, Murthy, K and Srinivasan, KK (2003) Naringin and naringenin inhibit nitrite-induced methemoglobin formation. Pharmazie 58: 564566.Google ScholarPubMed
Lee, DJ and Lee, JY (2004) Antioxidant activity by DPPH assay. Korean Journal of Crop Science 49: 187194.Google Scholar
Lee, CH, Jeong, TS, Choi, YK, Hyun, BH, Oh, GT, Kim, EH, Kim, JR, Han, JI and Bok, SH (2001) Antiatherogenic effect of citrus flavonoids, naringin and naringenin, associated with hepatic ACAT and aortic VCAM-1 and Mcp-1 in high cholesterol-fed rabbits. Biochemical and Biophysical Research Communications 284: 681688.CrossRefGoogle Scholar
Lee, MH, Yoon, S and Moon, JO (2004) The flavonoid naringenin inhibits dimethyl nitrosamine-induced liver damage in rats. Biological and Pharmaceutical Bulletin 27: 7276.CrossRefGoogle Scholar
Lee, M-H, Huh, D, Jo, D, Lee, G-D and Yoon, S-R (2007) Flavonoids components and functional properties of citrus peel hydrolysate. Journal of the Korean Society of Food Science and Nutrition 36: 13581364.CrossRefGoogle Scholar
Lien, TF, Yeh, HS and Su, WT (2008) Effect of adding extracted hesperetin, naringenin and pectin on egg cholesterol, serum traits and antioxidant activity in laying hens. Archives of animal nutrition 62: 3343.CrossRefGoogle Scholar
Lin, BQ and Chiou, GCY (2009) Antioxidant activity of naringenin on various oxidants induced damages in ARPE-19 cells and HUVEC. International Journal of Ophthalmology 2: 113117.Google Scholar
Malaveille, C, Hautefeuille, A, Pignatelli, B, Talaska, G, Vineis, P and Bartsch, H (1996) Dietary phenolics as anti-mutagens and inhibitors of tobacco-related DNA adduction in the urothelium of smokers. Carcinogenesis 17: 21932200.CrossRefGoogle ScholarPubMed
Mensor, LL, Menezes, FS, Leitao, GG, Reis, AS, dos Santos, TC, Coube, CS and Leitao, SG (2001) Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research 15: 127130.CrossRefGoogle Scholar
Middleton, E Jr, Kandaswami, C and Theoharides, TC (2000) The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacological Reviews 52: 67751.Google ScholarPubMed
Mikic, A, Mihailovic, V, Hauptvogel, P, Cupina, B, Petrovic, M, Krstic, D, Jovicic, D, Milosevic, B and Hauptvogel, R (2009) Wild populations of vetches (Vicia) as forage and green manure crops for temperate regions. Irish Journal of Agricultural and Food Research 48: 265.Google Scholar
Mossi, AJ, Cansian, RL, Carvalho, AZ, Dariva, C, Oliveira, JV, Mazutti, MA, Filho, NI and Echeverrigaray, S (2004) Extraction and characterization of volatile compounds in Maytenus ilicifolia, using high-pressure CO2 . Fitoterapia 75: 166178.CrossRefGoogle ScholarPubMed
Nijveldt, RJ, van Nood, E, van Hoorn, DE, Boelens, PG, van Norren, K and van Leeuwen, PA (2001) Flavonoids: a review of probable mechanisms of action and potential applications. American Journal Of Clinical Nutrition 74: 418425.Google ScholarPubMed
Nordberg, J and Arner, ESJ (2001) Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. Free Radical Biology and Medicine 31: 12871312.CrossRefGoogle ScholarPubMed
Olszewska, M (2007) Quantitative HPLC analysis of flavonoids and chlorogenic acid in the leaves and inflorescences of Prunus aerotina EHRH. Acta Chromatographica 19: 253269.Google Scholar
Patil, AB and Jadhav, AS (2013) Flavonoids an antioxidants: a review. International Journal of Pharmaceutical and Biological Sciences Research and Development 2: 720.Google Scholar
Perrino, P and Maruca, G (1989) Flavonoid taxonomic analysis of Vicia species of section Faba. Canadian Journal of Botany 67: 35293533.CrossRefGoogle Scholar
Re, R, Pellegrini, N, Proteggente, A, Pannala, A, Yang, M and Rice-Evans, C (1999) Antioxidant activity applying an improved ABTS radical cation decolorisation assay. Free Radical Biology and Medicine 26: 12311237.CrossRefGoogle Scholar
Stanojevic, L, Stankovic, M, Nikolic, V, Nikolic, L, Ristic, D, Canadanovic-Brunet, J and Tumbas, V (2009) Antioxidant activity and total phenolic and flavonoid contents of Hieracium pilosella L. extracts. Sensor 9: 57025714.CrossRefGoogle ScholarPubMed
Takahashi, T, Kobori, M, Shinmoto, H and Tsushida, T (1998) Structure–activity relationship of flavonoids and the induction of granulolytic or monocytic differentiation in HL 60 human myeloid leukemia cells. Bioscience, Biotechnology, and Biochemistry 62: 21992204.CrossRefGoogle Scholar
Van Sumere, CF, van de Casteele, K, de Loose, RE and Heursel, J (1985) Reversed phase HPLC analysis of flavonoids and the biochemical identification of cultivars of evergreen Azalea. In: Van Sumere, CF and Lea, PJ (eds) The Biochemistry of Plant Phenolics. Oxford, UK: Clarendon Press, pp. 1743.Google Scholar
Waterhouse, AL (2002) Determination of total phenolics. In: Wrolstad, RE (ed.) Current Protocols in Food Analytical Chemistry. New York: John Wiley & Sons, pp. 14.Google Scholar
Webb, M and Harborne, JB (1991) Leaf flavonoid aglycone patterns and sectional classification in the genus Vicia (Leguminosae). Biochemical Systematics and Ecology 19: 8186.CrossRefGoogle Scholar
Wildman, REC (2001) Nutraceuticals: a brief review of historical and teleological aspects. In: Wildman, REC (ed.) Handbook of Nutraceuticals and Functional Foods. Boca Raton, FL: CRC Press, pp. 112.Google Scholar
Willcox, JK, Ash, SL and Catignani, GL (2004) Antioxidants and prevention of chronic disease. Critical Reviews in Food Science and Nutrition 44: 275295.CrossRefGoogle ScholarPubMed
Winkel-Shirley, B (2001) Flavonoid biosynthesis. A colourful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiology 126: 485493.CrossRefGoogle Scholar
Winkel-Shirley, B (2002) Biosynthesis of flavonoids and effects of stress. Current Opinion in Plant Biology 5: 218223.CrossRefGoogle ScholarPubMed
Wojciechowski, MF (2003) Reconstructing the phylogeny of legumes (Leguminosae): an early 21st century perspective. In: Klitgaard, BB and Bruneau, A (eds) Advances in Legume Systematics. Kew, UK: Royal Botanic Gardens, pp. 535.Google Scholar
Young, ND, Mudgeand, J and Ellis, THN (2003) Legume genomes: more than peas in a pod. Current Opinion in Plant Biology 6: 199204.CrossRefGoogle ScholarPubMed

Lee supplementary material

Table S1

File 20 KB

Full text views

Full text views reflects PDF downloads, PDFs sent to Google Drive, Dropbox and Kindle and HTML full text views.

Total number of HTML views: 8
Total number of PDF views: 103 *
View data table for this chart

* Views captured on Cambridge Core between September 2016 - 23rd January 2021. This data will be updated every 24 hours.

Hostname: page-component-76cb886bbf-rm8z7 Total loading time: 0.793 Render date: 2021-01-23T03:06:42.469Z Query parameters: { "hasAccess": "0", "openAccess": "0", "isLogged": "0", "lang": "en" } Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": false, "newCiteModal": false }

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Comparison of flavonoid contents and antioxidant activities of Vicia species
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Comparison of flavonoid contents and antioxidant activities of Vicia species
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Comparison of flavonoid contents and antioxidant activities of Vicia species
Available formats
×
×

Reply to: Submit a response


Your details


Conflicting interests

Do you have any conflicting interests? *