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Ultrastructural characterization of host–parasite interactions of Plasmodium coatneyi in rhesus macaques

Published online by Cambridge University Press:  04 October 2021

E. D. Lombardini Open the ORCID record for E. D. Lombardini [Opens in a new window] ,
B. Malleret Open the ORCID record for B. Malleret [Opens in a new window] ,
A. Rungojn ,
N. Popruk ,
T. Kaewamatawong ,
A. E. Brown ,
G. D. H. Turner ,
B. Russell  and
D. J. P. Ferguson
E. D. Lombardini*
Affiliation:
Department of Veterinary Medicine, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand
B. Malleret
Affiliation:
Department of Microbiology and Immunology, Immunology Translational Research Programme, Yong Loo Lin School of Medicine, Immunology Programme, Life Sciences Institute, National University of Singapore, 117597 Singapore, Singapore Singapore Immunology Network (SIgN), Agency for Science & Technology, Singapore, Singapore
A. Rungojn
Affiliation:
Mahidol Oxford Clinical Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Nuffield Department of Medicine, Centre for Tropical Medicine, University of Oxford, Oxford, UK
N. Popruk
Affiliation:
Department of Veterinary Medicine, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand
T. Kaewamatawong
Affiliation:
Department of Veterinary Pathology, Chulalongkorn University, Bangkok, Thailand
A. E. Brown
Affiliation:
Faculty of Medical Technology, Mahidol University, Salaya, Thailand
G. D. H. Turner
Affiliation:
Mahidol Oxford Clinical Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Nuffield Department of Medicine, Centre for Tropical Medicine, University of Oxford, Oxford, UK
B. Russell
Affiliation:
Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand
D. J. P. Ferguson
Affiliation:
Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK Department Biological & Medical Sciences, Oxford Brookes University, Oxford, UK
*
Author for correspondence: E. D. Lombardini, E-mail: Eric.lombardini.mil@afrims.org
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Abstract

Plasmodium coatneyi has been proposed as an animal model for human Plasmodium falciparum malaria as it appears to replicate many aspects of pathogenesis and clinical symptomology. As part of the ongoing evaluation of the rhesus macaque model of severe malaria, a detailed ultrastructural analysis of the interaction between the parasite and both the host erythrocytes and the microvasculature was undertaken. Tissue (brain, heart and kidney) from splenectomized rhesus macaques and blood from spleen-intact animals infected with P. coatneyi were examined by electron microscopy. In all three tissues, similar interactions (sequestration) between infected red blood cells (iRBC) and blood vessels were observed with evidence of rosette and auto-agglutinate formation. The iRBCs possessed caveolae similar to P. vivax and knob-like structures similar to P. falciparum. However, the knobs often appeared incompletely formed in the splenectomized animals in contrast to the intact knobs exhibited by spleen intact animals. Plasmodium coatneyi infection in the monkey replicates many of the ultrastructural features particularly associated with P. falciparum in humans and as such supports its use as a suitable animal model. However, the possible effect on host–parasite interactions and the pathogenesis of disease due to the use of splenectomized animals needs to be taken into consideration.

Keywords

MalariaPlasmodium coatneyiPlasmodium falciparumrhesus macaquesequestrationultrastructure
Type
Research Article
Information
Parasitology , Volume 149 , Issue 2 , February 2022 , pp. 161 - 170
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Creative Commons
This is a work of the US Government and is not subject to copyright protection within the United States. Published by Cambridge University Press.
Copyright
Copyright © United States Army - Armed Forces Research Institute of Medical Sciences (AFRIMS), 2021

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