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Trypanosoma cruzi TcI and TcII transmission among wild carnivores, small mammals and dogs in a conservation unit and surrounding areas, Brazil

Published online by Cambridge University Press:  12 October 2012

FABIANA LOPES ROCHA ,
ANDRÉ LUIZ RODRIGUES ROQUE ,
RICARDO CORASSA ARRAIS ,
JEAN PIERRE SANTOS ,
VALDIRENE DOS SANTOS LIMA ,
SAMANTA CRISTINA DAS CHAGAS XAVIER ,
PEDRO CORDEIR-ESTRELA ,
PAULO SÉRGIO D'ANDREA  and
ANA MARIA JANSEN
FABIANA LOPES ROCHA
Affiliation:
Laboratório de Biologia de Tripanosomatídeos, Fundação Oswaldo Cruz, FIOCRUZ. Av. Brasil 4365. Pav. Rocha Lima 516. Rio de Janeiro-RJ. CEP: 21045-900, Brazil Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Fundação Oswaldo Cruz, FIOCRUZ. Av. Brasil 4365. Rio de Janeiro-RJ. CEP: 21045-900, Brazil Tríade – Instituto Brasileiro para Medicina da Conservação – Rua Silveira Lobo, 32, Caixa Postal 48, Bairro Casa Forte. Recife-PE. CEP: 52061-030, Brazil
ANDRÉ LUIZ RODRIGUES ROQUE
Affiliation:
Laboratório de Biologia de Tripanosomatídeos, Fundação Oswaldo Cruz, FIOCRUZ. Av. Brasil 4365. Pav. Rocha Lima 516. Rio de Janeiro-RJ. CEP: 21045-900, Brazil
RICARDO CORASSA ARRAIS
Affiliation:
Departamento de Medicina Preventiva e Saúde Animal. Universidade de São Paulo, USP. Av. Prof. Orlando Marques de Paiva, 87. Cidade Universitária. São Paulo – SP. CEP: 05508-270, Brazil
JEAN PIERRE SANTOS
Affiliation:
Instituto Pró-carnívoros. Av. Horácio Neto, 1030 – Parque Edmundo Zanoni. Atibaia-SP – CEP: 12945-010, Brazil
VALDIRENE DOS SANTOS LIMA
Affiliation:
Laboratório de Biologia de Tripanosomatídeos, Fundação Oswaldo Cruz, FIOCRUZ. Av. Brasil 4365. Pav. Rocha Lima 516. Rio de Janeiro-RJ. CEP: 21045-900, Brazil
SAMANTA CRISTINA DAS CHAGAS XAVIER
Affiliation:
Laboratório de Biologia de Tripanosomatídeos, Fundação Oswaldo Cruz, FIOCRUZ. Av. Brasil 4365. Pav. Rocha Lima 516. Rio de Janeiro-RJ. CEP: 21045-900, Brazil
PEDRO CORDEIR-ESTRELA
Affiliation:
Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Fundação Oswaldo Cruz, FIOCRUZ. Av. Brasil 4365. Rio de Janeiro-RJ. CEP: 21045-900, Brazil
PAULO SÉRGIO D'ANDREA
Affiliation:
Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Fundação Oswaldo Cruz, FIOCRUZ. Av. Brasil 4365. Rio de Janeiro-RJ. CEP: 21045-900, Brazil
ANA MARIA JANSEN*
Affiliation:
Laboratório de Biologia de Tripanosomatídeos, Fundação Oswaldo Cruz, FIOCRUZ. Av. Brasil 4365. Pav. Rocha Lima 516. Rio de Janeiro-RJ. CEP: 21045-900, Brazil
*
*Corresponding author: Laboratório de Biologia de Tripanosomatídeos, Fundação Oswaldo Cruz, FIOCRUZ. Av. Brasil 4365. Pav. Rocha Lima 516. Rio de Janeiro-RJ. CEP: 21045-900, Brazil. Fax: +55 21 2560 6572. E-mail: jansen@ioc.fiocruz.br
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Summary

Aiming to better understand the ecological aspects of Trypanosoma cruzi transmission cycles, wild carnivores, small mammals and dogs were examined for T. cruzi infection in the Serra da Canastra National Park region, Brazil. Isolates were genotyped using mini-exon gene and PCR-RFLP (1f8 and H3) genomic targets. Trypanosoma cruzi transmission was well established in the area and occurred in both wild and peridomestic environments. Dog seroprevalence was 29·4% (63/214) and TcI and TcII genotypes, besides mixed infections were observed. Only TcI was detected in wild mammals. Marsupials displayed lower relative abundance, but a high prevalence of positive haemocultures (4/22), whereas rodents displayed positive haemocultures (9/113) mainly in the abundant Akodon montensis and Cerradomys subflavus species. The felid Leopardus pardalis was the only carnivore to display positive haemoculture and was captured in the same region where the small mammal prevalence of T. cruzi infection was high. Two canid species, Chrysocyon brachyurus and Cerdocyon thous, were serologically positive for T. cruzi infection (4/8 and 8/39, respectively), probably related to their capacity to exploit different ecological niches. Herein, dog infection not only signals T. cruzi transmission but also the genotypes present. Distinct transmission strategies of the T. cruzi genotypes are discussed.

Keywords

transmission cyclestrophic networkreservoirDiscrete Typing UnitsChagas diseaseSerra da Canastra National ParkBrazil
Type
Research Article
Information
Parasitology , Volume 140 , Issue 2 , February 2013 , pp. 160 - 170
Copyright
Copyright © Cambridge University Press 2012

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