Hostname: page-component-848d4c4894-v5vhk Total loading time: 0 Render date: 2024-06-23T21:18:24.228Z Has data issue: false hasContentIssue false
  • English
  • Français

Trypanocidal activity of blood and tissue fluid from normal and infected rabbits treated with curative drugs

Published online by Cambridge University Press:  06 April 2009

L. G. Goodwin  and
Ellen D. Tierney
L. G. Goodwin
Affiliation:
Nuffield Institude of Comparative Medicine, Zoological Society of London, Regenťs Park, London N W1 4RY
Ellen D. Tierney
Affiliation:
Nuffield Institude of Comparative Medicine, Zoological Society of London, Regenťs Park, London N W1 4RY
Get access Rights & Permissions [Opens in a new window]

Extract

A new technique for determining the trypanocidal activity of body fluids has been devised, using very small quantities in the wells of plastic microtest plates.

The activities of blood and tissue fluid of rabbits have been measured after the injection of melarsoprol, suramin, diminazene aceturate and isometamidium chloride. Activity against Trypanosoma (Trypanozoon)brucei Persisted in body fluids for a shorter time in treated normal rabbits than in treated rabbits infected with the tryponosome. The difference was caused by the participation of the immune response of the infected animals.

The new technique was sensitive enough to detect these interactions of chemotherapy and immunology and should be of use in the study of new trypanocidal compounds.

Type
Research Article
Information
Parasitology , Volume 74 , Issue 1 , February 1977 , pp. 33 - 45
Copyright
Copyright © Cambridge University Press 1977

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Albert, A. (1965). In Selective Toxicity, 3rd ed., p. 76. London: Methuen; New York: Wiley.Google Scholar
Browning, C. H. (1908). Chemo-therapy in trypanosome infections: an experimental study. Journal of Pathology and Bacteriology 12, 166–90.Google Scholar
Chisholm, G. D., Waterworth, P. M., Calnan, J. G. & Garrod, L. P. (1973). Concentration of antibacterial agents in interstitial tissue fluid. British Medical Journal 1, 569–73.CrossRefGoogle ScholarPubMed
Gross, G. A. M. & Manning, J. C. (1973). Cultivation of Trypanosoma brucei spp. in semi-defined media. Parasitology 67, 315–31.Google Scholar
Ehrlich, P. & Shiga, K. (1904). Farbentherapeutische Versuche bei Trypanosomener-krankung. Berliner Klinische Wochenschrift 41, 362–5.Google Scholar
Goodwin, L. G. & Guy, M. W. (1973). Tissue fluid in rabbits infected with Trypanosoma (Trypanozoon) brucei. Parasitology 66, 499–513.CrossRefGoogle ScholarPubMed
Hawking, F. (1962). The concertration of melarsoprol (Mel B) and Mel W in plasma and cerebrospinal fluid estimated by bioassay with trypanosomes in vitro. Transactions of the Royal Society of Tropical Medicine and Hygiene 56, 354–63.CrossRefGoogle Scholar
Hawking, F. (1963). Experimental Chemotherapy, Vol. 1 (ed. Schnitzer, R. J. and Hawking, F.), p. 152. New York and London: Academic Press.Google Scholar
Hawking, F., Hennelly, T. J. & Quastel, J. H. (1937). Trypanocidal activity and arsenic content of the cerebrospinal fluid after administration of arsenic compounds. Journal of Pharamacology and Experimental Therapeutics 59, 157–75.Google Scholar
Isoun, M. J. & Isoun, T. T. (1974). The effects of HEPES buffered media on the in vitro cultivation of Trypanosoma vivax and T. brucei. Tropenmedizin and Parasitologie 25, 283–7.Google ScholarPubMed
King, H., Lourle, E. M. & Yorke, W. (1937). New trypanocidal substances. Lancet 2, 1360–3.CrossRefGoogle Scholar
Lanham, S. M. (1968). Separation of trypanosomes from the blood of infected rats and mice by anion-exchangers. Nature, London 218, 1273–4Google Scholar
Leonard, C. S. (1946). The immunizing effect in the action of trypanocidal agents. Science 104, 556.Google Scholar
Lourie, E. M. & Yorke, W. (1937). Studies in Chemotherapy. XVI. The trypanocidal action of synthalin. Annals of Trypical Medicine and Parasitology 31, 435–46.Google Scholar
Mayer, R. L. & Brousseau, D. (1946). Development of immunity to reinfection during chemoprophylaxis of trypanosomiasis with a new antimoney derivative. Proceedings of the Society for Experimental Biology and Medicine 62, 238–40.Google Scholar
Mitchell, G. H., Butcher, G. A., Voller, A. & Cohen, S. (1976). The effect of human immune LgG on the in vitro development of Plasmodium falciparum. Parasitology 72, 149–62.CrossRefGoogle Scholar
Phillips, R. S., Trigg, P. I., Scooti-Finnigan, T. J. & Bartholomew, R. K. (1972). Culture of Plasmodium falciparum in vitro; a sub-culture technique used for demonstrating antiplasmodial activity in serum from some Gambians, resident in an endemic malarious area. Parasitology 65, 525–35.CrossRefGoogle Scholar
Reiner, L. & Leonard, C. S. (1933). Studies on the mechanism of chemotherapeutic action. 11. The role of the ‘immunizing effect’ in the treatment of experimental trypanosomiasis with neoarsphenamine. Archives internationales de pharmacodynamie et de thérapie 44, 434–45.Google Scholar
Rollo, I. M., Williamson, J. & Lourie, E. M. (1949). Studies on the chemotherapy of melaminyl arsenicals and antimonials in Laboratory trypanosome infections. Annals of Tropical Medicine and Parasitology 43, 194–208.CrossRefGoogle ScholarPubMed
Schilling, C. (1909). Chemotherapeutische Versuche bei Trypanosomeinfektionen. Archiv für Schiffs- and Tropenhygiene 13, 1–18.Google Scholar
Schilling, C. & Jaffe, J. (1909). Weitere chemotherapeutische Versuche bei Trypanosomenkrankheiten. Archiv für Schiffs- und Troprnhygiene 13, 525–34.Google Scholar
Terry, B. T. (1911). Chemotherapeutic trypanosome studies with special reference to the immunity following cure. Monographs of the Rockefeller Institude, no. 3.Google Scholar
Voller, A., Bartlett, A. & Bidwell, D. E. (1976). Enzyme immunoassays for parasitic diseases. Transactions of the Royal Society of Tripical Medicine and Hygiene 70, 98–106.Google Scholar
Weinstein, L. (1975). In The Pharmacological Basis of Therapeutics, 5th ed. (ed. Goodman, L. S. and Gilman, A.), p. 1090. New York: Macmillan.Google Scholar
Yorke, W., Adams, A. R. D. & Murgatroyd, F. (1929). Studies in chemotherapy. I. A method for maintaining pathogenic trypanosomes alive in vitro at 37°C for 24 hours. Annals of Trypical Medicine and Parasitology 23, 501–18.CrossRefGoogle Scholar