Hostname: page-component-848d4c4894-nmvwc Total loading time: 0 Render date: 2024-07-05T20:19:22.828Z Has data issue: false hasContentIssue false
  • English
  • Français

Trichuris muris: structure and formation of the egg-shell

Published online by Cambridge University Press:  06 April 2009

C. M. Preston  and
T. Jenkins
C. M. Preston
Affiliation:
Department of Biological Sciences, Portsmouth Polytechnic, King Henry I Street, Portsmouth, Hants.
T. Jenkins
Affiliation:
Department of Biological Sciences, Portsmouth Polytechnic, King Henry I Street, Portsmouth, Hants.
Get access Rights & Permissions [Opens in a new window]

Summary

The development of the egg-shell of the trichuroid nematode, Trichuris muris, was observed by light and electron microscopy. Initial stages of shell formation occurred immediately following sperm entry into the oocyte in the spermatheca of the female worm. The external vitelline layer separated from the oolemma, reticulate oocyte granules were discharged into the resultant perivitelline space and stored glycogen contributed to the formation of the chitinous shell. A lipid layer developed between the inner surface of the chitinous layer and the oolemma. The fully formed egg-shell thus comprised an external vitelline layer, a middle chitinous layer and an inner lipid layer. The outer vitelline layer resembled a unit membrane with strands of particulate material attached to its outer surface. The middle chitinous layer was composed of chitin microfibrils encased in a protein coat; the sheets of microfibrils were arranged as parallel lamellae forming a helicoidal architecture. The inner lipid layer contained a variable number of loosely arranged strands of electron-opaque and less-opaque material.

Type
Research Article
Information
Parasitology , Volume 89 , Issue 2 , October 1984 , pp. 263 - 274
Copyright
Copyright © Cambridge University Press 1984

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anya, A. O. (1964). Studies on the structure of the female reproductive system and egg-shell formation in Aspiculuris tetraptera, Schulz, (Nematoda: Oxyuroidea). Parasitology 54, 117–28.CrossRefGoogle ScholarPubMed
Bird, A. F. & McClure, M. A. (1976). The tylenchid (Nematoda) egg shell: structure, composition and permeability. Parasitology 72, 19–28.CrossRefGoogle Scholar
Chitwood, B. G. & Chitwood, M. B. (1950). Introduction to Nematology. Baltimore: University Park Press.Google Scholar
Crompton, D. W. T. & Whitfield, P. J. (1974). Observations on the functional organisation of the ovarian balls of Moniliformis and Polymorphus (Acanthocephala). Parasitology 69, 429–30.CrossRefGoogle ScholarPubMed
Fairbairn, D. (1957). The biochemistry of Ascaris. Experimental Parasitology 6, 491–554.CrossRefGoogle ScholarPubMed
Favard, P. (1961). Évolution des ultrastructures cellulaires au cours de la spermatogenese de l' Ascaris. Annales des sciences naturelles, Zoologie 12, 53–152.Google Scholar
Foor, W. E. (1967). Ultrastructural aspects of oocyte development and shell formation in Ascaris lumbricoides. Journal of Parasitology 53, 1245–61.CrossRefGoogle ScholarPubMed
Foor, W. E. (1968). Zygote formation in Ascaris lumbricoides. Journal of Cell Biology 39, 119–34.CrossRefGoogle ScholarPubMed
Foor, W. E. (1970). Spermatozoon morphology and zygote formation in Nematodes. Biology of Reproduction Suppl. 2, 177–202.CrossRefGoogle ScholarPubMed
Foor, W. E. (1972). Origin and possible utilization of small dense granules in oocytes of Ascaris suum. Journal of Parasitology 58, 524–38.CrossRefGoogle ScholarPubMed
Grigonis, G. J. & Solomon, G. B. (1976). Capillaria hepatica: Fine structure of egg shell. Experimental Parasitology 40, 286–97.CrossRefGoogle ScholarPubMed
Hackman, R. H. (1955). Studies on chitin. 3. Adsorption of proteins to chitin. Australian Journal of Biological Science 8, 530–6.CrossRefGoogle Scholar
Hackman, R. H. & Goldberg, M. (1958). Proteins of the larval cuticle of Agrianome spinicollis (Coleoptera). Journal of Insect Physiology 2, 221–31.CrossRefGoogle Scholar
Harada, R., Maeda, T., Nakashima, A., Sadakaea, Y., Ando, M., Yonamine, K., Otsuji, Y. & Sato, H. (1970). Electronmicroscopial studies on the mechanism of oogenesis and fertilisation in Dirofilaria immitis. In Recent Advances in Researches on Filiariasis and Schistosomiasis in Japan, (ed. Sasa, M.), pp. 99–121. University of Tokyo Press and University Park Press.Google Scholar
Inatomi, S. (1960). Submicroscopic structure of the egg-shell of helminth. 11. A study on Trichuris vulpis. Acta Medicinae Okayama 14, 257–60.Google Scholar
Kochhar, D. M. (1960). Histochemical studies in the oogenesis and fertilisation of the nematode Porrocaecum angusticolle. Research Bulletin Punjab University 11, 207–19Google Scholar
Lee, C-C. (1975). Dirofilaria immitis: Ultrastructural aspects of oocyte development and zygote formation. Experimental Parasitology 37, 449–68.CrossRefGoogle ScholarPubMed
Lee, D. L. & Lešťan, P. (1971). Oogenesis and egg-shell formation in Heterakis gallinarum (Nematoda). Journal of Zoology 164, 189–96.CrossRefGoogle Scholar
McLaren, D. J. (1973). Oogenesis and fertilization in Dipetalonema viteae. (Nematoda: Filarioidea). Parasitology 66, 465–72.CrossRefGoogle Scholar
Monné, L. & Hönig, G. (1954). On the properties of the egg envelopes of the parasitic nematodes Trichuris and Capillaria. Arkiv för Zoologi 6, 559–62.Google Scholar
Neville, A. C. (1975). Biology of the Arthropod Cuticle. Berlin: Springer-Verlag.CrossRefGoogle Scholar
Perry, R. N., Wharton, D. A. & Clarke, A. J. (1982). The structure of the egg-shell of Globodera rostochienis (Nematoda: Tylenchida). International Journal for Parasitology 12, 481–5.CrossRefGoogle Scholar
Peura, R., Valtonen, E. T. & Crompton, D. W. T. (1982). The structure of the mature oocyte and zygote of Corynosoma semerme (Acanthocephala). Parasitology 84, 475–9.CrossRefGoogle Scholar
Preston, C. M. & Jenkins, T. (1983). Ultrastructural studies of early stages of oogenesis in a Trichuroid nematode, Trichuris muris. International Journal of Invertebrate Reproduction 6, 77–91.CrossRefGoogle Scholar
Romieu, M. (1911). La spermiogenèse chez l'Ascaris megalocephala. Archiv für Zellforschung 6, 254–325.Google Scholar
Wakelin, D. (1967). Acquired immunity to Trichuris muris in the albino laboratory mouse. Parasitology 57, 515–24.CrossRefGoogle ScholarPubMed
Wharton, D. A. (1979 a). The structure and formation of the egg-shell of Hammerschmidtiella diesingi. Hammerschmidt (Nematoda: Oxyuroidea). Parasitology 79, 1–12.CrossRefGoogle Scholar
Wharton, D. A. (1979 b). The structure and formation of the egg-shell of Syphacia obvelata Rudolphi (Nematoda: Oxyurida). Parasitology 79, 13–28.CrossRefGoogle ScholarPubMed
Wharton, D. A. (1980). Nematode egg-shells. Parasitology 81, 447–63.CrossRefGoogle ScholarPubMed
Wharton, D. A. & Jenkins, T. (1978). Structure and chemistry of the egg-shell of a nematode (Trichuris suis). Tissue and Cell 10, 427–40.CrossRefGoogle ScholarPubMed