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Triatoma infestans susceptibility to different Trypanosoma cruzi strains: parasite development and early escape from anterior midgut

Published online by Cambridge University Press:  17 September 2020

Larissa F. Paranaiba ,
Rodrigo P. Soares  and
Alessandra A. Guarneri Open the ORCID record for Alessandra A. Guarneri [Opens in a new window]
Larissa F. Paranaiba
Affiliation:
Instituto René Rachou, Fundação Oswaldo Cruz. Av. Augusto de Lima, 1715, 30.190-009, Belo Horizonte, Minas Gerais, Brazil Departamento de Parasitologia, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-901, Belo Horizonte, Minas Gerais, Brazil
Rodrigo P. Soares*
Affiliation:
Instituto René Rachou, Fundação Oswaldo Cruz. Av. Augusto de Lima, 1715, 30.190-009, Belo Horizonte, Minas Gerais, Brazil
Alessandra A. Guarneri*
Affiliation:
Instituto René Rachou, Fundação Oswaldo Cruz. Av. Augusto de Lima, 1715, 30.190-009, Belo Horizonte, Minas Gerais, Brazil
*
Authors for correspondence: Alessandra A. Guarneri, E-mail: alessandra.guarneri@fiocruz.br; Rodrigo P. Soares, E-mail: rodrigosoares28@hotmail.com
Authors for correspondence: Alessandra A. Guarneri, E-mail: alessandra.guarneri@fiocruz.br; Rodrigo P. Soares, E-mail: rodrigosoares28@hotmail.com
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Abstract

The escape kinetics from the anterior midgut (AM) of Trypanosoma cruzi during the initial steps of infection was assessed in Triatoma infestans, as well as its ability to survive migration in the digestive tract of the vector. All the four strains evaluated survived and reached variable parasite densities. After 49–50 days, YuYu [discrete typing units (DTU) I] strain reached the highest parasite numbers in the rectum followed by Bug (DTU V), CL-Brener (DTU VI) and Dm28c (DTU I). All strains accomplished metacyclogenesis. Bug strain reached the highest numbers of metacyclic trypomastigotes followed by YuYu and CL-Brener/Dm28c. A remarkable parasite reduction in the AM for Bug strain, but not Dm28c was noticed at 72 h of infection. In the posterior midgut + rectum high densities of parasites from both strains were detected at this period indicating the parasites crossed the AM. For Dm28c strain, in infections initiated with trypomastigotes, parasites left AM faster than those starting with epimastigotes. In conclusion, T. cruzi strains from different DTUs were able to infect T. infestans reaching variable parasite densities. The kinetics of migration in the digestive tract may be affected by strain and/or the evolutive form used for infection.

Keywords

Parasite developmentparasite–vector interactionstrainsTriatoma infestansTrypanosoma cruzi
Type
Research Article
Information
Parasitology , Volume 148 , Issue 3 , March 2021 , pp. 295 - 301
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Copyright
Copyright © The Author(s) 2020. Published by Cambridge University Press

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Footnotes

*

Those authors contributed equally to this manuscript.

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