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Transcripts of the multidrug resistance genes in chloroquine-sensitive and chloroquine-resistant Plasmodium falciparum

Published online by Cambridge University Press:  06 April 2009

R. M. Ekong ,
K. J. H. Robson ,
D. A. Baker  and
D. C. Warhurst
R. M. Ekong
Affiliation:
Medical Parasitology Department, London School of Hygiene and Tropical Medicine, Keppel Street, London WCIE 7HT
K. J. H. Robson
Affiliation:
MRC Molecular Haematology Unit, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DU
D. A. Baker
Affiliation:
Medical Parasitology Department, London School of Hygiene and Tropical Medicine, Keppel Street, London WCIE 7HT
D. C. Warhurst
Affiliation:
Medical Parasitology Department, London School of Hygiene and Tropical Medicine, Keppel Street, London WCIE 7HT
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Summary

Homologues of the mammalian multidrug resistance gene have been identified in isolates and clones of Plasmodium falciparum and designated pfmdr1 and pfmdr2. Mutations in pfmdr1 have been associated with chloroquine resistance but confirmation could not be obtained in a genetic cross. We have examined the copy number and expression of pfmdr1 and pfmdr2 in chloroquine-sensitive and -resistant P. falciparum and have found no relationship between the copy number of either gene and chloroquine resistance. However, a marked correlation was seen between levels of mRNA transcribed for each gene and chloroquine resistance. Two transcripts of pfmdr1 were detected, and in the asexual blood cycle an 8 kb transcript appeared first, followed by the appearance of a 7 kb species.

Keywords

Plasmodium falciparumchloroquine resistancepfmdr transcripts
Type
Research Article
Information
Parasitology , Volume 106 , Issue 2 , February 1993 , pp. 107 - 115
Copyright
Copyright © Cambridge University Press 1993

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