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Thymidine uptake by chicken kidney cells parasitized by Eimeria tenella: evidence for a diffusable mediating substance

Published online by Cambridge University Press:  06 April 2009

Catriona Urquhart
Catriona Urquhart
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London
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Summary

The pattern of DNA synthesis in chicken kidney (CK) cells parasitized by Eimeria tenella was altered; a 2-fold increase in the uptake of [3H]-thymidine occurred in these cells compared with that shown by the controls. This difference was significantly different at 16 h post-inoculation (p.i.) and reached a peak at 20 h p.i. Non-parasitized cells in co-culture with parasitized cells showed a level of incorporation intermediate between that of the parasitized and non-parasitized mono- cultures. It was shown that this stimulation was due to a factor released into the medium by the parasite or the parasitized cell. A possible course of events leading to induced and prolonged DNA synthesis by the host cell and the significance of this change in the life-cycle of the parasite is discussed.

Type
Research Article
Information
Parasitology , Volume 82 , Issue 2 , April 1981 , pp. 189 - 194
Copyright
Copyright © Cambridge University Press 1981

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References

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