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Studies on the free pool of amino acids of the cestode Hymenolepis diminuta

Published online by Cambridge University Press:  06 April 2009

L. H. Chappell  and
C. P. Read
L. H. Chappell
Affiliation:
Biology Department, Rice University, Houston, Texas, 77001, U.S.A.
C. P. Read
Affiliation:
Biology Department, Rice University, Houston, Texas, 77001, U.S.A.
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Extract

Alteration of the free amino acid pool of Hymenolepis diminuta was observed following incubation of worms with a single exogenous amino acid in vitro, recovery from which depended upon the initial magnitude of alteration. The free amino acid pool of both rat small intestine and H. diminuta was rendered imbalanced after feeding a single dietary dose of proline. Recovery was more rapid in the host than in the parasite tissues. The quantitative dynamics of amino acid change with time were examined. The effects of amino acid variation upon the uptake and incorporation of lysine-C14 into worm protein were examined in vitro. Four amino acids altered lysine incorporation; aspartate stimulated incorporation while ornithine, arginine and histidine reduced variously the absolute amount of lysine uptake but produced relative stimulation of lysine incorporation. These data indicate that, in the system examined, short term variation of the free amino acid pool is unlikely to affect protein synthesis in H. diminuta. Data were obtained suggesting the possible occurrence of certain amino acid inter conversions in H. diminuta.

Type
Research Article
Information
Parasitology , Volume 67 , Issue 3 , December 1973 , pp. 289 - 305
Copyright
Copyright © Cambridge University Press 1973

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References

REFERENCES

Arme, C. & Read, C. P. (1969). Fluxes of amino acids between the rat and a oestode symbiote. Comparative Biochemistry and Physiology 29, 1135–47.CrossRefGoogle Scholar
Bailey, G. N. A. (1971). Hymenolepis diminuta: circadian rhythm in movement and body length in the rat. Experimental Parasitology 29, 285–91.CrossRefGoogle ScholarPubMed
Campbell, J. W. (1963 a). Amino acids and nucleotides of the cestode, Hymenolepis diminuta. Comparative Biochemistry and Physiology 8, 181–5.CrossRefGoogle Scholar
Campbell, J. W. (1963 b). Urea formation and urea cycle enzymes in the cestode, Hymenolepis diminuta. Comparative Biochemistry and Physiology 8, 13–27.CrossRefGoogle ScholarPubMed
Chappell, L. H., Arai, H. P., Dike, S. C. & Read, C. P. (1970). Circadian migration of Hymenolepis (Cestoda) in the intestine. I. Observations on H. diminuta in the rat. Comparative Biochemistry and Physiology 34, 31–46.CrossRefGoogle Scholar
Cowie, D. B. (1962). Metabolic pools and the biosynthesis of protein. In Amino Acid Pools (ed. Holden, J. T.). Elsevier Publishing Co., Amsterdam.Google Scholar
Harris, B. G. & Read, C. P. (1968). Studies on membrane transport. III. Further characterization of amino systems in Hymenolepis diminuta (Cestoda). Comparative Biochemistry and Physiology 26, 545–52.CrossRefGoogle Scholar
Holmes, J. C. (1961). Effects of concurrent infections on Hymenolepis diminuta (Cestoda) and Moniliformis dubius (Acanthocephala). I. General effects and comparison with crowding. Journal of Parasitology 47, 209–16.CrossRefGoogle ScholarPubMed
Hopkins, C. A. (1969). The influence of dietary methionine on the amino acid pool of Hymenolepis diminuta in the rat's intestine. Parasitology 59, 407–27.CrossRefGoogle ScholarPubMed
Hopkins, C. A. & Callow, L. L. (1965). Methionine flux between a tapeworm (Hymenolepis diminuta) and its environment. Parasitology 55, 653–66.CrossRefGoogle ScholarPubMed
Hopkins, C. A. & Young, R. L. (1967). The effect of dietary amino acids on the growth of Hymenolepis diminuta. Parasitology 57, 705–17.CrossRefGoogle ScholarPubMed
Kilejian, A. Z. (1966). Permeation of L-proline in the cestode, Hymenolepis diminuta. Journal of Parasitology 52, 1108–15.CrossRefGoogle ScholarPubMed
Laws, G. F. & Read, C. P. (1969). Effect of the amino carboxy group on amino acid transport in Hymenolepis diminuta (Cestoda). Comparative Biochemistry and Physiology 30, 129–32.CrossRefGoogle Scholar
Mettrick, D. F. (1968). Studies on the protein metabolism of cestodes. 2. Effect of free dietary amino acid supplements on the growth of Hymenolepis diminuta. Parasitology 58, 37–45.CrossRefGoogle ScholarPubMed
Mettrick, D. F. (1970). Protein nitrogen, amino acid and carbohydrate gradients in the rat intestine. Comparative Biochemistry and Physiology 37, 517–41.CrossRefGoogle Scholar
Mettrick, D. F. & Munro, H. N. (1965). Studies on the protein metabolism of cestodes. 1. Effect of host dietary constituents on the growth of Hymenolepis diminuta. Parasitology 55, 453–66.CrossRefGoogle Scholar
Nasset, E. S. (1957). Role of the digestive tract in the utilisation of protein and amino acids. Journal of the American Medical Association 164, 172–7.CrossRefGoogle ScholarPubMed
Nasset, E. S., (1964). The nutritional significance of endogenous nitrogen excretion in nonruminants. In The Role of the Gastrointestinal Tract in Protein Metabolism (ed. Munro, H. N.). Blackwell: Oxford.Google ScholarPubMed
Nasset, E. S. & Ju, J. S. (1961). Mixture of endogenous and exogenous protein in the alimentary tract. Journal of Nutrition 74, 461–5.CrossRefGoogle Scholar
Prestcott, L. M. & Campbell, J. W. (1965). Phosphoenolpyruvate carboxylase activity and glycogenesis in the flatworm, Hymenolepis diminuta. Comparative Biochemistry and Physiology 14, 491–511.CrossRefGoogle Scholar
Ratner, S., Anslow, W. P. & Petrack, B. (1953). Biosynthesis of Urea VI. Enzymatic cleavage of argininosuccinic acid to arginine and fumaric acid. Journal of Biological Chemistry 204, 115–25.CrossRefGoogle ScholarPubMed
Read, C. P. (1967). Carbohydrate metabolism in Hymenolepis (Cestoda). Journal of Parasitology 53, 1023–9.CrossRefGoogle ScholarPubMed
Read, C. P. & Kilejian, A. Z. (1969). Circadian migratory behaviour of a cestode symbiote in the rat host. Journal of Parasitology 55, 574–8.CrossRefGoogle ScholarPubMed
Read, C. P., Rothman, A. H. & Simmons, J. E. (1963). Studies on membrane transport, with special reference to parasite-host integration. Annals of the New York Academy of Sciences 113, 154–205.CrossRefGoogle ScholarPubMed
Woodward, C. K. & Read, C. P. (1969). Studies on membrane transport VII. Transport of histidine through two distinct systems in the tapeworm Hymenolepis diminuta. Comparative Biochemistry and Physiology 30, 1161–77.CrossRefGoogle ScholarPubMed