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Some new methods for studying intestinal amoebae and other protozoa

Published online by Cambridge University Press:  06 April 2009

Clifford Dobell
Clifford Dobell
Affiliation:
National Institute for Medical Research, London, N.W. 3
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Extract

During the last few years I have successfully used a new method for investigating intestinal amoebae in cultures, and in the hope that it may also prove serviceable to other workers I now propose to describe it. At the same time I take the opportunity of noting two new staining methods which I have used especially in conjunction with this technique, but which are of general utility in protozoology and cytology.

Type
Research Article
Information
Parasitology , Volume 34 , Issue 1 , May 1942 , pp. 101 - 112
Copyright
Copyright © Cambridge University Press 1942

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References

1 Sterilization of thin coverglasses by dry heat or flaming is apt to warp them or damage their surfaces.

1 In an earlier paper, dealing with Dientamoeba fragilis (see Parasitology, 1940, 32, 417)Google Scholar, I noted (p. 431) the extreme difficulty of obtaining permanent preparations of the final stages of division of this organism by the usual methods. By the methods here described I have now obtained these with ease, and have readily confirmed my previous findings.

1 Described in earlier papers: see especially Parasitology, 1928, 20, 365.Google Scholar

2 Vide p. 109 infa.

1 As this is a chemical of variable and somewhat uncertain composition, I may note that I have hitherto always used that made by Merck. It is probably a phosphatodecatungstic acid of the general formula P2O5.24WO3. nH2O.

1 Cysts seldom survive for so long. The culture was made in the course of experiments to determine the viability of this strain, which has been fully described in earlier papers (Parasitology, 1936, 28, 541, and 1938, 30, 195).Google Scholar

1 The experiment has already been described elsewhere (Parasitology, 1933, 25, 436).Google Scholar