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Sequence variation of the Cytochrome b gene of various human infecting members of the genus Leishmania and their phylogeny

Published online by Cambridge University Press:  06 May 2004

G. E. LUYO-ACERO ,
H. UEZATO ,
M. OSHIRO ,
K. TAKEI ,
K. KARIYA ,
K. KATAKURA ,
E. GOMEZ-LANDIRES ,
Y. HASHIGUCHI  and
S. NONAKA
G. E. LUYO-ACERO
Affiliation:
Department of Dermatology, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0125, Japan
H. UEZATO
Affiliation:
Department of Dermatology, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0125, Japan
M. OSHIRO
Affiliation:
Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0125, Japan
K. TAKEI
Affiliation:
Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0125, Japan
K. KARIYA
Affiliation:
Division of Cell Biology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0125, Japan
K. KATAKURA
Affiliation:
Department of Parasitology, Gunma University School of Medicine 3-39-22 Showa-machi, Maebashi 371-8511, Japan
E. GOMEZ-LANDIRES
Affiliation:
Departmento de Medicina Tropical, Facultad de Medicina, Universidad Catolica Santiago de Guayaquil 10833, Guayaquil, Ecuador
Y. HASHIGUCHI
Affiliation:
Department of Parasitology, Kochi Medical School, Nankoku city, Kochi 783-8505, Japan
S. NONAKA
Affiliation:
Department of Dermatology, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0125, Japan
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Abstract

The Cytochrome b (Cyt b) gene has proved to be useful for identification and classification of many mammals and plants. In order to evaluate the utility of this gene for discrimination of Leishmania parasites as well as for exploring their phylogenetic relationships, we determined the nucleotide sequences of the Cyt b gene from 13 human-infecting Leishmania species (14 strains) from the New and Old Worlds. The Cyt b genes, approximately 1080 base pairs, were found to be A/T rich, and their 5′ terminal-editing regions were highly conserved. The nucleotide sequence variation among them was enough to discriminate parasite species; 245 nucleotide positions were polymorphic and 190 positions were parsimony informative. The phylogenetic relationships based on this gene, showed good agreement with the classification of Lainson & Shaw (1987) except for the inclusion of L. (L.) major in the L. (L.) tropica complex and the placement of L. tarentolae in another genus. These data show that the Cyt b gene is useful for phylogenetic study of Leishmania parasites.

Keywords

LeishmaniaCytochrome bphylogeny
Type
Research Article
Information
Parasitology , Volume 128 , Issue 5 , May 2004 , pp. 483 - 491
Copyright
2004 Cambridge University Press

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References

REFERENCES

BARNABE, C., BRISSE, S. & TIBAYRENC, M. (2003). Phylogenetic diversity of bat trypanosomes of subgenus Schizotrypanum based on multilocus enzyme electrophoresis, random amplified polymorphic DNA, and cytochrome b nucleotide sequences analyses. Infection, Genetics and Evolution 2, 201–208.CrossRefGoogle Scholar
BENNE, R. (1994). RNA editing in trypanosomes. European Journal of Biochemistry 221, 9–23.CrossRefGoogle Scholar
BREWSTER, S. & BARKER, D. C. (1999). The ATPase subunit 6 gene sequence predicts that RNA editing is conserved between lizard- and human-infecting Leishmania. Gene 235, 77–84.CrossRefGoogle Scholar
CROAN, D. G., MORRISON, D. A. & ELLIS, J. T. (1997). Evolution of the genus Leishmania revealed by comparison of DNA and RNA polymerase gene sequences. Molecular and Biochemical Parasitology 89, 149–159.CrossRefGoogle Scholar
CUPOLILLO, E., GRIMALDI, G. Jr., MOMEN, H. & BEVERLEY, S. M. (1995). Intergenic region typing (IRT): A rapid molecular approach to the characterization and evolution of Leishmania. Molecular and Biochemical Parasitology 73, 145–155.CrossRefGoogle Scholar
DEGLI ESPOSTI, M., DE VRIES, S., CRIMI, M., GHELLY, A., PATARNELLO, T. & MEYER, A. (1993). Mitochondrial cytochrome b: evolution and structure of the protein. Biochimica et Biophysica Acta 1143, 243–271.CrossRefGoogle Scholar
FARIAS, I. P., ORTI, G., SAMPAIO, I., SCHNEIDER, H. & MEYER, A. (2001). The cytochrome b gene as a phylogenetic marker: The limits of resolution for analyzing relationships among cichlid fishes. Journal of Molecular Evolution 53, 89–103.CrossRefGoogle Scholar
FEAGIN, J. E., SHAW, J. M., SIMPSON, L. & STUART, K. (1988). Creation of AUG initiation codons by addition of uridines within cytochrome b transcripts of kinetoplastids. Proceedings of the National Academy of Sciences, USA 85, 539–543.CrossRefGoogle Scholar
FERNANDES, O., MURTHY, V. K., KURATH, U., DEGRAVE, W. M. & CAMPBELL, D. A. (1994). Mini-exon gene variation in human pathogenic Leishmania species. Molecular and Biochemical Parasitology 66, 261–271.CrossRefGoogle Scholar
GRIMALDI, G. Jr., DAVID, J. R. & McMAHON-PRATT, D. (1987). Identification and distribution of new world Leishmania species characterized by serodeme analysis using monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 36, 270–287.CrossRefGoogle Scholar
HOWELL, N. (1989). Evolutionary conservation of protein regions in the proton-motive cytochrome b and their possible roles in redox catalysis. Journal of Molecular Evolution 29, 157–169.CrossRefGoogle Scholar
IRWIN, D. M., KOCHER, T. D. & WILSON, A. C. (1991). Evolution of the cytochrome b gene of mammals. Journal of Molecular Evolution 32, 128–144.CrossRefGoogle Scholar
KREUTZER, R. D. & CHRISTENSEN, H. A. (1980). Characterization of Leishmania spp. by isozyme electrophoresis. American Journal of Tropical Medicine and Hygiene 29, 199–208.CrossRefGoogle Scholar
KUMAR, S., TAMURA, K., JAKOBSEN, I. B. & NEI, M. (2001). MEGA2: Molecular evolutionary genetics analyfsis software. Bioinformatics 17, 1244–1245.CrossRefGoogle Scholar
LAINSON, R. & SHAW, J. J. (1987). Evolution, classification and geographical distribution. In Leishmaniasis in Biology and Medicine (ed. Peters, W. & Killick-Kendrik, R. ), pp. 1–120. Academic Press, London.
LANDWEBER, L. F. & GILBERT, W. (1994). Phylogenetic analysis of RNA editing: A primitive genetic phenomenon. Proceedings of the National Academy of Sciences, USA 91, 918–921.CrossRefGoogle Scholar
MACHADO, C. A. & AYALA, F. J. (2001). Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of Trypanosoma cruzi. Proceedings of the National Academy of Sciences, USA 98, 7396–7401.CrossRefGoogle Scholar
MASLOV, D. A., AVILA, H. A., LAKE, J. A. & SIMPSON, L. (1994). Evolution of RNA editing in kinetoplastid protozoa. Nature, London 368, 345–347.CrossRefGoogle Scholar
MEREDITH, S. E. O., ZIJLSTRA, E. E., SCHOONE, G. J., KROON, C. C. M., VAN EYS, G. J. J. M., SCHAEFFER, K. U., EL-HASSAN, A. M. & LAWYER, P. G. (1993). Development and application of the polymerase chain reaction for the detection and identification of Leishmania parasites in clinical material. Archives de l'Institut Pasteur de Tunis 70, 419–431.Google Scholar
STUART, K. (1983). Kinetoplast DNA, mitochondrial DNA with a difference. Molecular and Biochemical Parasitology 9, 93–110.CrossRefGoogle Scholar
TAMURA, K. & NEI, M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10, 512–526.Google Scholar
TIBAYRENC, M., KJELLBERG, F. & AYALA, F. (1990). A clonal theory of parasitic protozoa: The population structures of Entamoeba, Giardia, Leishmania, Nigeria, Plasmodium, Trichomonas and Trypanosoma and their medical and taxonomical consequences. Proceedings of the National Academy of Sciences, USA 87, 2414–2418.CrossRefGoogle Scholar
ULIANA, S. R. B., AFFONSO, M. H. T., CAMARGO, E. P. & FLOETER-WINTER, L. M. (1991). Leishmania: Genus identification based on a specific sequence of the 18S ribosomal RNA sequence. Experimental Parasitology 72, 157–163.CrossRefGoogle Scholar
VAN EYS, G. J. J. M. & MEREDITH, S. E. O. (1996). Detection and characterization of Leishmania parasites by DNA-based methods. In Methods in Molecular Biology (ed. Clapp, J. P.), pp. 227–242. Humana Press Inc, NJ.
VAN EYS, G. J. J. M., SCHOONE, G. J., KROON, N. C. M. & EBELING, S. B. (1992). Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites. Molecular and Biochemical Parasitology 51, 133–142.Google Scholar
WORLD HEALTH ORGANIZATION (2000). WHO Information Fact Sheet N-116. Available from URL: http://www.who.int//inf-fs/en/fact116.html.
YURCHENKO, V., KOLESNIKOV, A. & LUKES J. (2000). Phylogenetic analysis of Trypanosomatina (Protozoa: Kinetoplastida) based on minicircle conserved regions. Folia Parasitologica 47, 1–5.CrossRefGoogle Scholar