Skip to main content Accessibility help
×
Home

Septata intestinalis frequently isolated from stool of AIDS patients with a new cultivation method

  • T. Van Gool (a1), E. U. Canning (a2), H. Gilis (a1), M. A. Van Den Bergh Weerman (a3), J. K. M. Eeftinck Schattenkerk (a4) and J. Dankert (a1)...

Summary

Two species of microsporidia, Enterocytozoon bieneusi and Septata intestinalis have been reported as intestinal parasites of AIDS patients. In attempts to establish E. bieneusi in vitro, spores were concentrated from stool samples from 4 AIDS patients with biopsy-proven E. bieneusi infections. After sterilization of the concentrate in antibiotic solution, the spores were added to monolayers of RK13 cells grown on the membranes of Transwells. Cultures were established from 7 stool samples from the 4 patients but in every case the species established was S. intestinalis not E. bieneusi. On retrospective examination of the stools, a very small number of spores of a size comparable to that of S. intestinalis was found but this species was not detected in biopsies. Typical septate vacuoles containing Type I tubules were observed in vitro but in contrast to the original description, meronts were intravacuolar and sporogony was mainly disporoblastic. The cultivation system, used for the first time for microsporidia, revealed the presence of unsuspected S. intestinalis infections and indicates that this species may be much more common than hitherto suspected. S. intestinalis has not previously been cultured.

Copyright

References

Hide All
Blanshard, B., Hollister, W. S., Peacock, D. G., Tovey, D. G., Ellis, D. S., Canning, E. U. & Gazzard, B. G. (1992). Simultaneous infection with two types of intestinal microsporidia in a patient with AIDS. Gut 33, 418–20.
Cali, A. (1971). Morphogenesis in the genus Nosema. Proceedings of the VIth International Colloquium on Insect Pathology, Maryland, 431–8.
Cali, A., Kotler, D. P. & Orenstein, J. M. (1993). Septata intestinalis n.g., n. sp., an intestinal microsporidian associated with chronic diarrhea and dissemination in AIDS patients. Journal of Eukaryotic Microbiology 40, 101–12.
Canning, E. U., Curry, A., Lacey, C. J. N. & Fenwick, J. D. (1992). Ultrastructure of Encephalitozoon sp. infecting the conjunctival, corneal and nasal epithelia of a patient with AIDS. European Journal of Protistology 28, 226–37.
Canning, E. U., Field, A. S., Hing, M. C. & Marriott, D. J. (1994). Further observations on the ultrastructure of Septata intestinalis. European Journal of Protistology (in the Press).
Canning, E. U. & Hollister, W. S. (1990). Enterocytozoon bieneusi (Microspora): prevalence and pathogenicity in AIDS patients. Transactions of tile Royal Society of Tropical Medicine and Hygiene 84, 181–6.
Canning, E. U., Hollister, W. S., Colbourn, N. I., Curry, A. & Gobel, U. B. (1993). Human microsporidioses: site specificity, prevalence and species identification. AIDS 7 (Suppl. 3), S3–S7.
Desportes, I. (1976). Ultrastructure de Stempellia mutabiles Léger et Hesse microsporidie parasite de l'éphémère Ephemera vulgata. Protistologica 12, 121–50.
Desser, S. S., Hong, H. & Yang, Y. J. (1992). Ultrastructure of the development of a species of Encephalitozoon cultured from the eye of an AIDS patient. Parasitology Research 78, 677–83.
Didier, E. S., Didier, P. J., Friedberg, D. N., Stenson, S. M., Orenstein, J. M., Yee, R. W., Tio, F. O., Davis, R. M., Vossbrink, C., Millichamp, N. & Shadduck, J. A. (1991 a) Isolation and characterization of a new human microsporidian, Encephalitozoon hellem (n.sp.) from three AIDS patients with keratoconjunctivitis. Journal of Infectious Diseases 163, 617–21.
Didier, P. J., Didier, E. S., Orenstein, J. M. & Shadduck, J. A. (1991 b). Fine structure of a new human microsporidian, Encephalitozoon hellem, in culture. Journal of Protozoology 38, 502–7.
Eeftinck Schattenkerk, J. K. M., Van Gool, T., Van Ketel, R. J., Bartelsman, J. F. W. M., Kuiken, C. L., Terpstra, W. J. & Reiss, P. (1991). Clinical significance of small-intestinal microsporidiosis in HIV-1-infected individuals. Lancet 337, 895–8.
Field, A. S., Canning, E. U., Hing, M. C., Verre, J. & Marriott, D. J. (1993). Microsporidia in HIV-infected patients in Sydney, Australia, a report of 37 cases, a new diagnostic technique and the light microscopy and ultrastructure of a disseminated species. AIDS 7 (Suppl. 3), S27S33.
Hartskeerl, R. A., Schuitema, A. R. J., Van Gool, T. & Terpstra, W. J. (1993). Genetic evidence for the occurrence of extra-intestinal Enterocytozoon bieneusi infections. Nucleic Acids Research 21, 4150.
Hollister, W. S., Canning, E. U. & Willcox, A. (1991). Evidence for widespread occurrence of antibodies to Encephalitozoon cuniculi (Microspora) in man provided by ELISA and other serological tests. Parasitology 102, 3343.
Hollister, W. S., Canning, E. U., Colbourn, N. I., Curry, A. & Lacey, C. J. N. (1993). Characterization of Encephalitozoon hellem (Microspora) isolated from the nasal mucosa of a patient with AIDS. Parasitology 107, 351–8.
Loubès, C., Maurand, J. & Ormières, R. (1979). Étude ultrastructurale de Spraguea lophii (Doflein, 1898), microsporidie parasite de la boudroie: essai d'interprétation du dimorphisme sporal. Protistologica 15, 4354.
Orenstein, J. M., Chiang, J., Steinberg, W., Smith, P. D., Rotterdam, H. & Kotler, D. P. (1990). Intestinal microsporidiosis as a cause of diarrhoea in human immunodeficiency virus-infected patients: a report of 20 cases. Human Pathology 21, 475–81.
Orenstein, J. M., Tenner, M. B. A., Cali, A. & Kotler, D. P. (1992). A microsporidian previously undescribed in humans, infecting enterocytes and macrophages, and associated with diarrhea in an Acquired Immunodeficiency Syndrome patient. Human Pathology 23, 722–8.
Schuitema, A. R. J., Hartskeerl, R. A., Van Gool, T., Laxminarayan, R. & Terpstra, W. J. (1993). Application of the polymerase chain reaction for the diagnosis of microsporidiosis. AIDS 7 (Suppl. 3), S57–S61.
Shadduck, J. A. (1969). Nosema cuniculi: in vitro isolation. Science 166, 516–17.
Sprague, V. & Vernick, S. H. (1971). The ultrastructure of Encephalitozoon cuniculi (Microsporidia, Nosematidae) and its taxonomic significance. Journal of Protozoology 18, 560–9.
Takvorian, P. M. & Cali, A. (1983). Appendages associated with Glugea stephani, a microsporidian found in flounder. Journal of Protozoology 30, 251–6.
Van Gool, T., Canning, E. U. & Dankert, J. (1994). An improved practical and sensitive technique for the detection of Microsporidian spores in stool samples. Transactions of Tropical Medicine and Hygiene 88, 189–90.
Van Gool, T., Hollister, W. S., Eeftinck Schattenkerk, J. K. M., Van Den Bergh Weerman, M. A., Terpstra, W. J., Van Ketel, R. J., Reiss, P. & Canning, E. U. (1990). Diagnosis of Enterocytozoon bieneusi microsporidiosis in AIDS patients by recovery of spores from faeces. Lancet 336, 697–8.
Van Gool, T., Snijders, F., Reiss, P., Eeftinck Schattenkerk, J. K. M., Van Den Bergh Weerman, M. A., Bartelsman, J. F. W. M., Bruins, J. J. M., Canning, E. U. & Dankert, J. (1993). Diagnosis of intestinal and disseminated microsporidial infections in HIV-infected individuals by a new rapid fluorescent technique. Journal of Clinical Pathology 46, 694–9.
Vávra, J. (1976). Structure of the Microsporidia. In Biology of the Microsporidia, Comparative Pathobiology, Vol. 1 (ed. Bulla, L. A. & Cheng, T. C.), pp. 185. New York: Plenum Press.
Vávra, J., Bedrnik, P. & Cinatl, J. (1972). Isolation and in vitro cultivation of the mammalian microsporidian Encephalitozoon cuniculi. Folia Parasitologica 19, 349–54.
Visvesvara, G. S., Leitch, G. T., Gorelkin, L., Wilcox, M., Weber, R. & Bryan, R. T. (1993). Short-term in vitro culture of Enterocytozoon bieneusi from four different patients with AIDS. 9th International Conference on AIDS,Berlin. Abstract no. PO-B10–1447, 376.
Visvesvara, G. S., Leitch, G. J., Moura, H., Wallace, S., Weber, R. & Brian, R. T. (1991). Culture, electron microscopy, and immunoblot studies on a microsporidian parasite isolated from the urine of a patient with AIDS. Journal of Protozoology 38, 1058–118.
Vossbrinck, C. R., Baker, M. D., Didier, E. S., Debrunner-vosbrinck, B. A. & Shadduck, J. A. (1993). Ribosomal DNA sequences of Encephalitozoon hellem and Encephalitozoon cuniculi: species identification and phytogenetic construction. Journal of Eukaryotic Microbiology 40, 354–62.
Weber, R., Bryan, R. T., Owen, R. L., Wilcox, C. M., Gorelkin, L. & Visvesvara, G. S. (1992). Improved light-microscopical detection of microsporidia spores in stool and duodenal aspirates. New England Journal of Medicine 326, 161–6.

Keywords

Septata intestinalis frequently isolated from stool of AIDS patients with a new cultivation method

  • T. Van Gool (a1), E. U. Canning (a2), H. Gilis (a1), M. A. Van Den Bergh Weerman (a3), J. K. M. Eeftinck Schattenkerk (a4) and J. Dankert (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed