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Schistosoma japonicum: immunolocalization of paramyosin during development

Published online by Cambridge University Press:  01 January 1997

G. N. GOBERT ,
D. J. STENZEL ,
M. K. JONES ,
D. E. ALLEN  and
D. P. McMANUS
G. N. GOBERT
Affiliation:
Analytical Electron Microscopy Facility, Queensland University of Technology, GPO Box 2434, George Street, Brisbane, Queensland 4001, Australia
D. J. STENZEL
Affiliation:
Analytical Electron Microscopy Facility, Queensland University of Technology, GPO Box 2434, George Street, Brisbane, Queensland 4001, Australia
M. K. JONES
Affiliation:
Centre for Microscopy and Microanalysis, The University of Queensland, St Lucia, Brisbane, Queensland 4072, Australia
D. E. ALLEN
Affiliation:
Analytical Electron Microscopy Facility, Queensland University of Technology, GPO Box 2434, George Street, Brisbane, Queensland 4001, Australia
D. P. McMANUS
Affiliation:
Molecular Parasitology Unit, Tropical Health Program, The Queensland Institute of Medical Research, 300 Herston Road, Herston, Brisbane, Queensland 4029, Australia
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Abstract

This paper describes the localization of paramyosin immunoreactivity in Schistosoma japonicum and represents the first comparative immunolocalization study among schistosome adult, cercariae and lung schistosomula by electron microscopy. A polyclonal antibody was utilized to immunolabel paramyosin or paramyosin-like proteins. Paramyosin was localized within the muscle layer of all 3 developmental stages. Furthermore, paramyosin was localized within granules of the post-acetabular glands of cercariae, and within the tegument matrix and surface of lung schistosomules. Adults and cercariae did not display any detectable paramyosin on the surface or within the tegument. The possible functions of paramyosin within S. japonicum and the relevance of these findings in relation to the reported protective properties of paramyosin as an anti-schistosome vaccine target molecule are discussed.

Keywords

Schistosoma japonicumimmunocytochemistrytegumentparamyosinconfocal microscopyelectron microscopy
Type
Research Article
Information
Parasitology , Volume 114 , Issue 1 , January 1997 , pp. 45 - 52
Copyright
© 1997 Cambridge University Press

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