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Sandwich dot-immunogold filtration assay (DIGFA) for specific immunodiagnosis of active neuroangiostrongyliasis

Published online by Cambridge University Press:  02 October 2020

Praphathip Eamsobhana Open the ORCID record for Praphathip Eamsobhana [Opens in a new window] ,
Anchalee Tungtrongchitr ,
Hoi-Sen Yong ,
Anchana Prasartvit ,
Darawan Wanachiwanawin  and
Xiao-Xian Gan
Praphathip Eamsobhana*
Affiliation:
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Anchalee Tungtrongchitr
Affiliation:
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Hoi-Sen Yong
Affiliation:
Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
Anchana Prasartvit
Affiliation:
Department of Disease Control, Ministry of Public Health, Nonthaburi, Thailand;
Darawan Wanachiwanawin
Affiliation:
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Xiao-Xian Gan
Affiliation:
Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, P.R. China
*
Authors for correspondence: Praphathip Eamsobhana, E-mail: praphathip.eam@mahidol.ac.th; Anchalee Tungtrongchitr, E-mail: anchalee@tun@mahidol.ac.th
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Abstract

Serological tests may yield false-negative results for specific antibodies detection before or at the early seroconversion phase. Tests that detect circulating antigens of Angiostrongylus cantonensis would therefore be of value in diagnosis to distinguish current or past infection. Here, a quick, easy to perform, portable and inexpensive diagnostic device for detection of 31-kDa A. cantonensis specific antigens had been developed. This sandwich dot-immunogold filtration assay (AcDIGFAAg), for detecting active angiostrongyliasis was produced using anti-A. cantonensis polyclonal antibody dotted on the nitrocellulose membrane as a capture agent and colloidal gold-labelled anti-31 kDa A. cantonensis antibody as a detection agent. A well-defined pink dot, indicating positivity, was seen readily by naked eye within 10–15 min. The AcDIGFAAg detected A. cantonensis-specific antigens in cerebrospinal fluid samples from 4 out of 10 serologically confirmed angiostrongyliasis cases and 2 out of 5 suspected cases with negative anti-A. cantonensis antibodies. Among the 19 patient sera with A. cantonensis infection, 2 showed positive reaction by AcDIGFAAg. No positive AcDIGFAAg reaction was observed in all the serum samples with other parasitic diseases, and the healthy controls. The present ‘AcDIGFAAg’ enables rapid qualitative detection of the specific 31-kDa antigens of A. cantonensis in clinical samples with potential for application even under resource-limited settings.

Keywords

Angiostrongylus cantonensissandwich dot-immunogold filtration assayDIGFAantigen detection31-kDa antigenactive angiostrongyliasis
Type
Research Article
Information
Parasitology , Volume 148 , Special Issue 2: 6th International Workshop on Angiostrongylus and Angiostrongyliasis, Hilo, Hawai'i, USA, January, 2020 , February 2021 , pp. 234 - 239
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Copyright
Copyright © The Author(s) 2020. Published by Cambridge University Press

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