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Proteomics and the Trypanosoma brucei cytoskeleton: advances and opportunities

Published online by Cambridge University Press:  04 April 2012

NEIL PORTMAN  and
KEITH GULL
NEIL PORTMAN
Affiliation:
The Sir William Dunn School of Pathology and Oxford Centre for Integrative Systems Biology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
KEITH GULL*
Affiliation:
The Sir William Dunn School of Pathology and Oxford Centre for Integrative Systems Biology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
*
*Author for correspondence: Professor Keith Gull, The Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK. Tel.: +44 (0)1865285455. E-mail: keith.gull@path.ox.ac.uk
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Summary

Trypanosoma brucei is the etiological agent of devastating parasitic disease in humans and livestock in sub-saharan Africa. The pathogenicity and growth of the parasite are intimately linked to its shape and form. This is in turn derived from a highly ordered microtubule cytoskeleton that forms a tightly arrayed cage directly beneath the pellicular membrane and numerous other cytoskeletal structures such as the flagellum. The parasite undergoes extreme changes in cellular morphology during its life cycle and cell cycles which require a high level of integration and coordination of cytoskeletal processes. In this review we will discuss the role that proteomics techniques have had in advancing our understanding of the molecular composition of the cytoskeleton and its functions. We then consider future opportunities for the application of these techniques in terms of addressing some of the unanswered questions of trypanosome cytoskeletal cell biology with particular focus on the differences in the composition and organisation of the cytoskeleton through the trypanosome life-cycle.

Keywords

Trypanosoma bruceicytoskeletonproteomicflagellumflagella connectorBilobePFR
Type
Research Article
Information
Parasitology , Volume 139 , Issue 9: Symposia of the British Society for Parasitology Volume 48 Proteomic insights into parasite biology , August 2012 , pp. 1168 - 1177
Copyright
Copyright © Cambridge University Press 2012

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