Skip to main content Accessibility help

Proteomic analysis of a 120 kDa protein complex in cyst fluid of Taenia solium metacestode and preliminary evaluation of its value for the serodiagnosis of neurocysticercosis

  • E.-G. LEE (a1), Y.-A. BAE (a1), Y.-T. JEONG (a1), J.-Y. CHUNG (a2), E.-Y. JE (a1), S.-H. KIM (a1), B.-K. NA (a1), J.-W. JU (a3), T.-S. KIM (a3), L. MA (a4), S.-Y. CHO (a1) and Y. KONG (a1)...


Cyst fluid (CF) of Taenia solium metacestode (TsM) is an important source of serodiagnostic antigens. We have investigated the molecular characteristics of the 120 kDa protein complex in TsM CF purified by fast performance liquid chromatography. The structure of the purified protein was characterized by a variety of proteomic analyses. The protein was found to consist of 2 major components of 42–46 and 22–28 kDa, and shared 3 subunits of 14, 16 and 18 kDa. The 42–46 kDa component was determined to contain 3 additional subunits of 22, 28 and 38 kDa. These 6 subunits were shown to originate from either the 14 or 18 kDa precursor. We assessed the antibody reactivity of the native protein, its individual subunits and the recombinant 14 and 18 kDa proteins, and found that the 120 kDa protein, particularly 14 and 18 kDa subunits revealed high reliability for differentiation of active and mixed stage NC from chronic NC. The subunits of the 120 kDa protein complex identified herein represent some of the low-molecular weight glycoproteins which have been described in several previous studies. Recognizing and understanding the structural and immunological relationship of these proteins will facilitate the development of new serodiagnostic assays.


Corresponding author

Department of Molecular Parasitology, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea. Tel: +82 31 299 6251. Fax: +82 31 299 6269. E-mail:


Hide All


Baranwal, A. K., Singhi, P. D., Khandelwal, N. and Singhi, S. C. ( 1998). Albendazole therapy in children with focal seizures and single small enhancing computerized tomographic lesions: a randomized, placebo-controlled, double blind trial. Pediatric Infectious Diseases Journal 17, 696700.
Bern, C., Garcia, H. H., Evans, C., Gonzalez, A. E., Verastegui, M., Tsang, V. C. and Gilman, R. H. ( 1999). Magnitude of the disease burden from neurocysticercosis in a developing country. Clinical Infectious Diseases 29, 12031209.
Blum, H., Beier, H. and Grass, H. J. ( 1987). Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8, 9399.
Carpio, A., Escobar, A. and Hauser, W. ( 1998). Cysticercosis and epilepsy: a critical review. Epilepsia 39, 10251040.
Cho, S. Y., Kim, S. I., Kang, S. Y., Choi, D. Y., Suk, J. S., Choi, K. S., Ha, Y. S., Chung, C. S. and Myung, H. J. ( 1986). Evaluation of enzyme-linked immunosorbent assay in serological diagnosis of human neurocysticercosis using paired samples of serum and cerebrospinal fluid. Korean Journal of Parasitology 24, 2541.
Cho, S. Y., Kim, S. I., Kang, S. Y. and Kong, Y. ( 1988). Biochemical properties of a purified protein in cystic fluid of Taenia solium metacestodes. Korean Journal of Parasitology 26, 8794.
Chung, J. Y., Bahk, Y. Y., Huh, S., Kang, S. Y., Kong, Y. and Cho, S. Y. ( 1999). A recombinant 10 kDa protein of Taenia solium metacestodes specific to active neurocysticercosis. Journal of Infectious Diseases 180, 13071315.
Del Brutto, O. H., Rajshekhar, V., White, A. C. Jr., Tsang, V. C., Nash, T. E., Takayanagui, O. M., Schantz, P. M., Evans, C. A., Flisser, A., Correa, D., Botero, D., Allan, J. C., Sarti, E., Gonzalez, A. E., Gilman, R. H. and Garcia, H. H. ( 2001). Proposed diagnostic criteria for neurocysticercosis. Neurology 57, 177183.
Del Brutto, O. H., Sotelo, J. and Roman, G. C. ( 1998). Neurocysticercosis: a Clinical Handbook. 1st Edn. Swets and Zeitlinger Publishers, Lisse.
Eom, K. S., Jeon, H. K., Kong, Y., Hwang, U. W., Yang, Y., Li, X., Xu, L., Feng, Z., Pawlowski, Z. S. and Rim, H. J. ( 2002). Identification of Taenia asiatica in China: molecular, morphological, and epidemiological analysis of a Luzhai isolate. Journal of Parasitology 88, 758764.
Escobar, A. ( 1983). The pathology of neurocysticercosis. In Cysticercosis of the Central Nervous System ( ed. Palacio, E., Rodriguez-Cabajal, J. and Taveras, J. M.), pp. 2754. Thomas, Springfield, USA.
Garcia, H. H., Evans, C. A., Nash, T. E., Takayanagui, O. M., White, A. C. Jr., Botero, D., Rajshekhar, V., Tsang, V. C., Schantz, P. M., Allan, J. C., Flisser, A., Correa, D., Sarti, E., Friedland, J. S., Martinez, S. M., Gonzalez, A. E., Gilman, R. H. and Del Brutto, O. H. ( 2002). Current consensus guidelines for treatment of neurocysticercosis. Clinical Microbiology Review 15, 747756.
Garcia, H. H., Gonzalez, A. E., Gilman, R. H. and Cysticerosis Working Group in Peru. ( 2003). Diagnosis, treatment and control of Taenia solium cysticercosis. Current Opinion in Infectious Diseases 16, 411419.
Garcia, H. H., Pretell, E. J., Gilman, R. H., Martinez, S. M., Moulton, L. H., Del Brutto, O. H., Herrera, G., Evans, C. A., Gonzalez, A. E. and Cysticercosis Working Group in Peru. ( 2004). A trial of antiparasitic treatment to reduce the rate of seizures due to cerebral cysticercosis. New England Journal of Medicine 350, 249258.
Görg, A., Obermaier, C., Boguth, G., Harder, A., Scheibe, B., Wildgruber, R. and Weiss, W. ( 2000). The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 21, 10371053.
Greene, R. M., Hancock, K., Wilkins, P. P. and Tsang, V. C. ( 2000). Taenia solium: molecular cloning and serologic evaluation of 14- and 18-kDa related, diagnostic antigens. Journal of Parasitology 86, 10011007.
Greene, R. M., Wilkins, P. P. and Tsang, V. C. ( 1999). Diagnostic glycoproteins of Taenia solium cysts share homologous 14- and 18-kDa subunits. Molecular and Biochemical Parasitology 99, 257261.
Hancock, K., Khan, A., Williams, F. B., Yushak, M. L., Pattabhi, S., Noh, J. and Tsang, V. C. ( 2003). Characterization of the 8-kilodalton antigens of Taenia solium metacestodes and evaluation of their use in an enzyme-linked immunosorbent assay for serodiagnosis. Journal of Clinical Microbiology 41, 25772586.
Haslam, S. M., Restrepo, B. I., Obregon-Henao, A., Teale, J. M., Morris, H. R. and Dell, A. ( 2003). Structural characterization of the N-linked glycans from Taenia solium metacestodes. Molecular and Biochemical Parasitology 126, 103107.
Ito, A., Plancarte, A., Ma, L., Kong, Y., Flisser, A., Cho, S. Y., Liu, Y. H., Kamhawi, S., Lightowlers, M. W. and Schantz, P. M. ( 1998). Novel antigens for neurocysticercosis: simple method for preparation and evaluation for serodiagnosis. American Journal of Tropical Medicine and Hygiene 59, 291294.
Kim, S. I., Kang, S. Y., Cho, S. Y., Hwang, E. S. and Cha, C. Y. ( 1986). Purification of cystic fluid antigen of Taenia solium metacestodes by affinity chromatography using monoclonal antibody and its antigenic characterization. Korean Journal of Parasitology 24, 145158.
Ko, R. C. and Ng, T. F. ( 1998). Purification of larval Taenia solium antigens by isoelectric focusing. Veterinary Parasitology 74, 191202.
Landry, F., Lombardo, C. R. and Smith, J. W. ( 2000). A method for application of samples to matrix-assisted laser desorption ionization time-of-flight targets that enhances peptide detection. Analytical Biochemistry 279, 18.
Obregon-Henao, A., Gil, D. L., Gomez, D. I., Sanzon, F., Teale, J. M. and Restrepo, B. I. ( 2001). The role of N-linked carbohydrates in the antigenicity of Taenia solium metacestode glycoproteins of 12, 16 and 18 kD. Molecular and Biochemical Parasitology 114, 209215.
Page, R. D. ( 1996). Tree view: an application to display phylogenetic trees on personal computers. Computerized Applied Biosciences 12, 357358.
Palacio, L. G., Jimenez, I., Garcia, H. H., Jimenez, M. E., Sanchez, J. L., Noh, J., Ahn, L., Mora, O., Giraldo, M., Tsang, V. C. The Neuroepidemiological Research Group of Antioquia. ( 1998). Neurocysticercosis in persons with epilepsy in Medellin, Colombia. Epilepsia 39, 13341339.
Phiri, I. K., Ngowi, H., Afonso, S., Matenga, E., Boa, M., Mukaratirwa, S., Githigia, S., Saimo, M., Sikasunge, C., Maingi, N., Lubega, G. W., Kassuku, A., Michael, L., Siziya, S., Krecek, R. C., Noormahomed, E., Vilhena, M., Dorny, P. and Willingham, A. L. 3rd. ( 2003). The emergence of Taenia solium cysticercosis in Eastern and Southern Africa as a serious agricultural problem and public health risk. Acta Tropica 87, 1323.
Restrepo, B. I., Obregon-Henao, A., Mesa, M., Gil, D. L., Ortiz, B. L., Mejia, J. S., Villota, G. E., Sanzon, F. and Teale, J. M. ( 2000). Characterisation of the carbohydrate components of Taenia solium metacestode glycoprotein antigens. International Journal for Parasitology 30, 689696.
Roman, G., Sotelo, J., Del Brutto, O. H., Flisser, A., Dumas, M., Wadia, N., Botero, D., Cruz, M., Garcia, H. H., De Bittencourt, P. R., Trelles, L., Arriagada, C., Lorenzana, P., Nash, T. E. and Spina-Franca, A. ( 2000). A proposal to declare neurocysticercosis an international reportable disease. Bulletin of the World Health Organization 78, 399406.
Sako, Y., Nakao, M., Ikejima, T., Piao, X. Z., Nakaya, K. and Ito, A. ( 2000). Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes. Journal of Clinical Microbiology 38, 44394444.
Tsang, V. C., Brand, J. A. and Boyer, A. E. ( 1989). An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). Journal of Infectious Diseases 159, 5059.
Vilhena, M., Santos, M. and Torgal, J. ( 1999). Seroprevalence of human cysticercosis in Maputo, Mozambique. American Journal of Tropical Medicine and Hygiene 61, 5962.
Villota, G. E., Gomez, D. I., Volcy, M., Franco, A. F., Cardona, E. A., Isaza, R., Sanzon, F., Teale, J. M. and Restrepo, B. I. ( 2003). Similar diagnostic performance for neurocysticercosis of three glycoprotein preparations from Taenia solium metacestodes. American Journal of Tropical Medicine and Hygiene 68, 276280.
White, A. C. Jr., Robinson, P. and Kuhn, R. ( 1997). Taenia solium cysticercosis: host-parasite interactions and immune response. Chemical Immunology 66, 209230.
White, A. C. Jr. ( 2000). Neurocysticercosis: updates on epidemiology, pathogenesis, diagnosis, and management. Annual Review of Medicine 51, 187206.
Yang, H. J., Chung, J. Y., Yun, D. H., Kong, Y., Ito, A., Ma, L., Liu, Y. H., Lee, S. C., Kang, S. Y. and Cho, S. Y. ( 1998). Immunoblot analysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes. Parasite Immunology 20, 483488.



Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed