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Proteinases released in vitro by the parasitic stages of Teladorsagia circumcincta, an ovine abomasal nematode

  • C. J. Young (a1), J. B. McKeand (a2) and D. P. Knox (a1)

Summary

Proteinases released during in vitro maintenance of third (L3) and fourth larval stage (L4) and adult Teladorsagia circumcincta (formerly Ostertagia circumcincta), an ovine abomasal nematode parasite, were characterized on the basis of pH optima, molecular size and specific proteinase inhibitor sensitivity. Enzyme activity was maximal at alkaline pH and stage-specific release was demonstrated. Proteinases released by the adult parasite degraded a variety of protein substrates including plasminogen, albumin and haemoglobin, in a pH-dependent manner. At alkaline pH fibrinogen degradation was restricted to the α and β peptide chains although the γ peptide chain was also degraded at acidic pH. Inhibitor sensitivity studies indicated that degradation was predominantly due to metalloproteinases although aspartyl proteinase activity was indicated at acidic pH.

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Beynon, R. J. & Salvesen, G. (1989). Commercially available proteinase inhibitors. In Proteolytic Enzymes; a Practical Approach (ed. Beynon, R. J. & Bond, J. S.), p. 241. Oxford: IRL Press.
Britton, C., Knox, D. P., Canto, G. J., Urquhart, G. M. & Kennedy, M. W. (1992). The secreted and somatic proteinases of the bovine lungworm Dictyocaulus viviparus and their inhibition by antibody from infected and vaccinated animals. Parasitology 105, 325–33.
Brophy, P. M. & Pritchard, D. I. (1992). Immunity to helminths: ready to tip the biochemical balance? Parasitology Today 8, 419–22.
Chapman, C. B. & Mitchell, G. F. (1982). Proteolytic cleavage of immunoglobulin by enzymes released by Fasciola hepatica. Veterinary Parasitology 11, 165–78.
de Cock, H., Knox, D. P., Claerebout, E. & De Graff, D. C. (1993). Partial characterisation of proteolytic enzymes in different developmental stages of Ostertagia ostertagi. Journal of Helminthology 67, 271–8.
Healer, J., Ashall, F. & Maizels, R. M. (1991). Characterisation of proteolytic enzymes from larval and adult Nippostrongylus brasiliensis. Parasitology 103, 305–14.
Hotez, P. J., Trang, N., McKerrow, J. H. & Cerami, A. (1985). Isolation and characterisation of a proteolytic enzyme from the adult hookworm Ancylostoma caninum. Journal of Biological Chemistry 260, 7343–8.
Howell, R. M. (1966). Collagenase activity of immature Fasciola hepatica. Nature 209, 713–14.
Knox, D. P. & Jones, D. J. (1990). Studies on the presence and release of proteolytic enzymes (proteinases) in gastro-intestinal nematodes of ruminants. International Journal for Parasitology 20, 243–9.
Knox, D. P. & Kennedy, M. W. (1988). Proteinases released by the parasitic larval stages of Ascaris suum and their inhibition by antibody. Molecular and Biochemical Parasitology 28, 207–16.
Knox, D. P., Redmond, D. L. & Jones, D. G. (1993). Characterisation of proteinases in extracts of adult Haemonchus contortus, the ovine abomasal nematode. Parasitology 106, 395404.
Knox, D. P. & Schallig, H. (1994). Characterisation of the secreted proteinases of adult Haemonchus contortus, the ovine abomasal nematode. British Society of Parasitology, Spring Meeting, Abstract p. 86.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 681–5.
Leid, R. W., Suquet, C. M. & Tanigoshi, L. (1987). Parasite defence mechanisms for the evasion of host immune responses. Veterinary Parasitology 25, 147–62.
Maki, J. & Yanagisawa, T. (1986). Demonstration of carboxyl and thiol protease activities in adult Schistosoma mansoni, Dirofilaria immitis, Angiostrongylus cantonensis and Ascaris suum. Journal of Helminthology 60, 31–7.
Matthews, B. E. (1977). The passage of larval helminths through tissue barriers. In Parasite Invasion (ed. Taylor, A. E. R. & Muller, R.), pp. 93119. Oxford: Blackwell Scientific Publications.
Matthews, B. E. (1984). The source, release and specificity of proteolytic enzyme activity produced by Anisakis simplex larvae (Nematoda: Ascaridida) in vitro. Journal of Helminthology 58, 175–85.
McKerrow, J. H. & Doenhoeff, M. J. (1988). Schistosome proteinases. Parasitology Today 4, 334–9.
Robertson, B. D., Bianco, A. E., McKerrow, J. H. & Maizels, R. M. (1989). Toxocara canis: proteolytic enzymes secreted by infective larvae in vitro. Experimental Parasitology 69, 30–6.
Smith, A. M., Dowd, A. J., McGonigle, S., Keegan, P. S., Brennan, G., Trudgett, A. & Dalton, J. P. (1993). Purification of a Cathepsin L-like proteinase secreted by adult Fasciola hepatica. Molecular and Biochemical Parasitology 62, 18.
Smith, W. D. (1988). Mechanisms of immunity to gastrointestinal nematodes of sheep. In Increasing Small Ruminant Productivity in Semi-arid Areas (ed. Thomson, E. F. & Thomson, F. S.), pp. 275285. Netherlands: ICARDA.
Thomas, R. J. & Waller, P. J. (1975). Significance of serum pepsinogen and abomasal pH levels in a field infection of Ostertagia circumcincta in lambs. Veterinary Record 97, 468–71.
Thorson, R. E. (1956). Proteolytic activity in extracts of the oesophagus of Ancylostoma caninum and the effect of immune sera on the activity. Journal of Parasitology 42, 21–5.

Keywords

Proteinases released in vitro by the parasitic stages of Teladorsagia circumcincta, an ovine abomasal nematode

  • C. J. Young (a1), J. B. McKeand (a2) and D. P. Knox (a1)

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