Hostname: page-component-84b7d79bbc-2l2gl Total loading time: 0 Render date: 2024-07-30T14:22:21.974Z Has data issue: false hasContentIssue false
  • English
  • Français

Propagation of Cystosporogenes operophterae (Microspora), a microsporidian parasite of the winter moth, Operophtera brumata, in a Spodoptera frugiperda cell line

Published online by Cambridge University Press:  06 April 2009

Wei-Dong Xie
Wei-Dong Xie
Affiliation:
Department of Pure and Applied Biology, Imperial College, London SW7 2BB
Get access Rights & Permissions [Opens in a new window]

Summary

Cystosporogenes operophterae, a microsporidian parasite of the winter moth, Operophera brumata, was cultured in a Spodoptera frugiperda cell line. The infected cultures were achieved by addition of haemolymph from larvae infected with C. operophterae. The infection rates for the cell line ranged from 80 to 95% after incubation for 10 days at 25 or 20 °C. There was no development in cultures incubated at 30 °C. Light and electron microscopy were used to observe the development of C. operophterae in vitro.

Type
Research Article
Information
Parasitology , Volume 97 , Issue 2 , October 1988 , pp. 229 - 237
Copyright
Copyright © Cambridge University Press 1988

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Canning, E. U. (1981). Encephalitozoon lacertae n. sp., a microsporidian parasite of the lizard Podarcis muralis. In Parasitological Topics, Society of Protozoologists Special Publication No. 1, pp. 57–64. (ed. Canning, E. U.). Kansas: Allen Press.Google Scholar
Canning, E. U., Barker, R. J., Nicholas, J. P. & Page, A. M. (1985). The ultrastructure of three microsporidia from winter moth, Operophtera brumata (L.), and the establishment of a new genus Cystosporogenes n.g. for Pleistophora operophterae (Canning, 1960). Systematic Parasitology 7, 213–15.Google Scholar
Ishihara, R. (1967). Stimuli causing extrusion of polar filaments of Glugea fumiferanae spores. Canadian Journal Microbiology 13, 1321–32.Google Scholar
Ishihara, R. (1968). Growth of Nosema bombycis in primary cell cultures of mammalian and chicken embryos. Journal of Invertebrate Pathology 11, 328–9.CrossRefGoogle Scholar
Ishihara, R. & Sohi, S. S. (1966). Infection of ovarian tissue culture of Bombyx mori by Nosema bombycis spores. Journal of Invertebrate Pathology 8, 538–40.CrossRefGoogle Scholar
Kawarabata, T. & Ishihara, R. (1984). Infection and development of Nosema bombycis (Microsporida: Protozoa) in a cell line of Antheraea eucalypti. Journal of Invertebrate Pathology 44, 52–62.CrossRefGoogle Scholar
Kurtti, T. J. & Brooks, M. A. (1977). The rate of development of a microsporidian in moth cell culture. Journal of Invertebrate Pathology 29, 126–32.Google Scholar
Kurtti, T. J., Tsang, R. R. & Brooks, M. A. (1984). The spread of infection by the microsporidan, Nosema disstriae, in infected cell lines. Journal of Protozoology 30, 625–57.Google Scholar
Loubès, C., Maurand, J., Gasc, C., de Buron, I. & Barral, J. (1984). Etude ultrastructurale de Loma dimorpha n. sp., microsporidie parasite de poissons gobiidae languedociens. Protistologica 20, 579–89.Google Scholar
Malone, L. A. (1984). Factors controlling in vitro hatching of Vairimorpha plodiae (Microspora) spores and their infectivity to Plodia interpunctella, Heliothis virescens, and Pieris brassicae. Journal of Invertebrate Pathology 44, 192–7.CrossRefGoogle Scholar
Pakes, S. P., Shadduck, J. A. & Cali, A. (1975). Fine structure of Encephalitozoon cuniculi from rabbits, mice and hamsters. Journal of Protozoology 22, 481–3.Google Scholar
Singh, P. (1977). Artificial Diets for Insects, Mites, and Spiders, 1st Ed. New York: Plenum.Google Scholar
Smith, J. E., Barker, R. J. & Lai, P. F. (1982). Culture of microsporidia in vertebrate cells. Parasitology 85, 427–35.Google Scholar
Sohi, S. S. & Wilson, G. G. (1976). Persistent infection of Malacasoma disstria (Lepidoptera: Lasiocampidae) cell cultures with Nosema (Glugea) disstriae (Microsporidia: Nosematidae). Canadian Journal of Zoology 54, 336–42.Google Scholar
Streett, D. A., Ralph, D. A. & Hink, W. F. (1980). Replication of Nosema algerae in three insect cell lines. Journal of Protozoology 27, 113–17.Google Scholar
Trager, W. (1937). The hatching of spores of Nosema bombycis Nägeli and the partial development of the organism in tissue cultures. Journal of Parasitology 23, 226–7.Google Scholar
Undeen, A. H. (1975). Growth of Nosema algerae in pig kidney cell cultures. Journal of Protozoology 22, 107–10.Google Scholar
Undeen, A. H. (1978). Spore hatching in some Nosema species with particular reference to N. algerae Vávra and Undeen. Miscellaneous Publications of the Entomological Society of America 11, 29–49.Google Scholar
Vaughn, J. L., Goodwin, R. H., Tompkins, G. J. & McCawley, P. (1977). The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro 13, 213–17.CrossRefGoogle ScholarPubMed
Weidner, E. (1975). Interaction between Encephalitozoon cuniculi and macrophages. Zeitschrift für Parasitenkunde 47, 1–9.Google Scholar
Wilson, G. G. & Sohi, S. S. (1977). Effect of temperature on healthy and microsporidia-infected continuous culture of Malacosoma disstria haemocytes. Canadian Journal of Zoology 55, 713–17.Google Scholar