Skip to main content Accessibility help
×
Home

Pomphorhynchus laevis (Acanthocephala): elevation of haemolymph protein concentrations in the intermediate host, Gammarus pulex (Crustacea: Amphipoda)

  • C. R. Bentley (a1) and H. Hurd (a1)

Summary

Comparisons were made between the protein concentrations and protein profiles of uninfected Gammarus pulex and those infected with cystacanths of Pomphorhynchus laevis. Comparisons of the extractable haemolymph volumes from uninfected and infected G. pulex, when related to host tissue weight, showed that there was no difference in the host tissue/haemolymph volume ratio between infected and uninfected individuals. SDS-PAGE of haemolymph samples resolved 36 bands of protein, the major components being band 6(19 kDa) and 21(33 kDa). Densitometric analysis of haemolymph samples showed that infected gammarids had total protein concentrations elevated by 84%. The (88 kDa) subunit was identified as the monomer of the respiratory protein haemocyanin. This band was significantly elevated in infected individuals (one-way ANOVA P < 0.001); increasing from 30 to 45% of the total haemolymph proteins.

Copyright

References

Hide All
Adelung, D. (1971). Untersuchungen zur Häutungphysiologie der decapoden Krebse am Beispiel der Strandkrabbe Carcinus maenas. Helgoländer wissenschaftliche Meeresuntersuchungen 22, 66119.
Bancroft, F. C., Terwilliger, R. C. & Van Holde, K. E. (1966). Investigations of the subunit structure of Limulus haemocyanin. Biological Bulletin 131, 384.
Bethel, W. H. & Holmes, J. C. (1973). Altered evasive behaviour and response to light in amphipods harbouring acanthocephalan cystacanths. Journal of Parasitology 59, 945–56.
Bethel, W. H. & Holmes, J. C. (1977). Increased vulnerability of amphipods to predation owing to altered behaviour induced by larval acanthocephalans. Canadian Journal of Zoology 55, 110–6.
Brown, A. F. & Thompson, D. B. A. (1986). Parasite manipulation of host behaviour: acanthocephalans and shrimps in the laboratory. Journal of Biological Education 20, 121–7.
Camp, J. W. & Huizinga, H. W. (1979). Altered color, behaviour and predation susceptibility of the isopod Ascellus intermedius infected with Acanthocephalus dirus. Journal of Parasitology 65, 667–9.
Chan, H. M. & Weeks, J.M. (1992). The subunit structural composition of amphipod haemocyanin (Crustacea; Amphipoda; Talitridae). Comparative Biochemistry and Physiology 101B, 567–72.
Dezfuli, B. S., Zanini, N., Reggiani, G. & Rossi, R. (1991). Echinogammarus stammeri (Amphipoda) as an intermediate host for Pomphorhynchus laevis (Acanthocephala) parasite of fishes from the River Brenta. Bollettino di Zoologica 58, 267–71.
Djangmah, J. S. (1970). The effects of feeding and starvation on copper in the blood and hepatopancreas, and on the blood proteins of Crangon vulgaris (Fabricius). Comparative Biochemistry and Physiology 32, 709–31.
Helluy, S. & Holmes, J. C. (1990). Serotonin, octopamine, and the clinging behaviour induced by the parasite Polymorphus minutus (Acanthocephala) in Gammarus lacustris (Crustacea). Canadian Journal of Zoology 68, 1214–20.
Kennedy, C. R., Broughton, P. F. & Hine, P. M. (1978). The status of brown and rainbow trout, Salmo trutta and S. gairdneri as hosts of the acanthocephalan Pomphorhynchus laevis. Journal of Fish Biology 13, 265–87.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.
Manwell, C. & Baker, C. M. A. (1963). Starch gel electrophoresis of some sera from some marine Arthropods: studies on the heterogeneity of haemocyanins. Comparative Biochemistry and Physiology 8, 193208.
Moore, J. (1983). Responses of an avian predator and its isopod prey to an acanthocephalan parasite. Ecology 64, 1000–15.
Rumpus, A. E. & Kennedy, C. R. (1974). The effect of the acanthocephalan Pomphorhychus laevis upon the respiration of its intermediate host, Gammarus pulex. Parasitology 68, 271–84.
Uglow, R. F. (1969). Haemolymph protein concentrations in portunid crabs. III. The effect of Sacculina. Comparative Biochemistry and Physiology 31, 969–73.
Whittaker, J. R. (1959). Localization of haemocyanin on starch gel electrophoretic patterns. Nature, London 184, 193–4.

Keywords

Pomphorhynchus laevis (Acanthocephala): elevation of haemolymph protein concentrations in the intermediate host, Gammarus pulex (Crustacea: Amphipoda)

  • C. R. Bentley (a1) and H. Hurd (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed