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Phospholipase A1 from Trypanosoma cruzi infective stages generates lipid messengers that activate host cell protein kinase c

Published online by Cambridge University Press:  23 November 2006

M. L. BELAUNZARÁN ,
M. J. WAINSZELBAUM ,
E. M. LAMMEL ,
G. GIMENEZ ,
M. M. ALOISE ,
J. FLORIN-CHRISTENSEN  and
E. L. D. ISOLA
M. L. BELAUNZARÁN
Affiliation:
Department of Microbiology, School of Medicine, University of Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina
M. J. WAINSZELBAUM
Affiliation:
Department of Cell Biology and Physiology, Washington University, School of Medicine, 660 S. Euclid Avenue, Box 8228, St Louis, MO 63110 USA
E. M. LAMMEL
Affiliation:
Department of Cell Biology and Physiology, Washington University, School of Medicine, 660 S. Euclid Avenue, Box 8228, St Louis, MO 63110 USA
G. GIMENEZ
Affiliation:
Department of Microbiology, School of Medicine, University of Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina
M. M. ALOISE
Affiliation:
Department of Microbiology, School of Medicine, University of Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina
J. FLORIN-CHRISTENSEN
Affiliation:
Department of Microbiology, School of Medicine, University of Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina
E. L. D. ISOLA
Affiliation:
Department of Microbiology, School of Medicine, University of Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina
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Abstract

Here we have studied phospholipase A1 (Plase A1) from Trypanosoma cruzi infective stages and it's possible role regarding the interaction with mammalian host cells. Plase A1 was mainly detected as a membrane-bound activity in the infective amastigote and trypomastigote stages, being remarkably higher with respect to the non-infective epimastigotes. It is noteworthy that only the infective stages secreted Plase A1. Moreover, along the differentiation process from epimastigotes into metacyclic trypomastigotes, the secreted enzyme activity increased simultaneously with the appearance of metacyclic forms, as expected. Since this enzyme is predominantly membrane-associated and secreted by the infective stages, Vero cell lipid profile modifications were analysed after interaction with either intact infective parasites or purified T. cruzi Plase A1. Significant changes in Vero cell lipid composition were observed, with the appearance of free fatty acids, diacylglycerol and lysophosphatidylcholine. Concomitantly with the generation of second lipid messengers, host cell protein kinase C activation was demonstrated. These results indicate that T. cruzi Plase A1 could play a critical role in the early events of parasite-host cell interaction that precede invasion.

Keywords

phospholipase A1protein kinase CTrypanosoma cruziparasite-host cell interaction
Type
Research Article
Information
Parasitology , Volume 134 , Issue 4 , April 2007 , pp. 491 - 502
Copyright
© 2006 Cambridge University Press

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