Skip to main content Accessibility help
×
Home

Phenotypic characterization of Haemonchus contortus: a study of isolates from Sweden and Kenya in experimentally infected sheep

  • K. TROELL (a1), C. TINGSTEDT (a1) and J. HÖGLUND (a1)

Abstract

The effects of cold storage of infective third-stage larvae (L3) of different isolates of the parasitic nematode Haemonchus contortus were studied with respect to infectivity, pre-patent period and propensity for larval arrestment. Two complementary experiments were conducted with 2 groups of lambs, each animal being inoculated with 2000 L3 of either Swedish or Kenyan origin. In a first experiment, L3s were cold treated at 5 °C for 9 months prior to infection, whereas in a second experiment larvae were newly hatched. Individual faecal egg counts (FECs), and worm burdens were determined for each experiment. The results showed that the greatest differences were associated with the pre-treatment of larvae. The pre-patent period and the FECs differed significantly between the experiments but not between the isolates used in each experiment. However, the extent of hypobiosis was significantly different between the two isolates when fresh larvae were used (36% Kenyan isolate and 70% Swedish). The storage of H. contortus at 5 °C had no apparent effect on the infectivity of L3s, as high establishment ranging from 43 to 74% were observed, irrespective of isolates used. This study showed that H. contortus exhibited similar phenotypic traits regardless of geographical origin. Thus, there was limited evidence for adaptations to temperate climatic conditions.

Copyright

Corresponding author

Department of Parasitology, Swedish University of Agricultural Sciences, 751 89 Uppsala, Sweden. Tel: +46 18 67 40 00. Fax: +46 18 30 91 62. E-mail: Johan.Hoglund@bvf.slu.se

References

Hide All

REFERENCES

Allonby, E. W. and Urquhart, G. M. ( 1975). The epidemiology and pathogenic significance of haemonchosis in a merino flock in east Africa. Veterinary Parasitology 1, 129143.
Blitz, N. M. and Gibbs, H. C. ( 1971). Morphological characterization of the stage of arrested development of Haemonchus contortus in sheep. Canadian Journal of Zoology 47, 991995.
Blitz, N. M. and Gibbs, H. C. ( 1972). Studies on the arrested development of Haemonchus contortus in sheep. I. The induction of arrested development. International Journal for Parasitology 2, 512.
Capitini, L. A., McClure, K. E. and Herd, R. P. ( 1990). Effect of environmental stimuli on pre-infective and infective stages of Haemonchus contortus in the northern united states for the induction of hypobiosis. Veterinary Parasitology 35, 281293.
Connan, R. M. ( 1971). The seasonal incidence of inhibition of development in Haemonchus contortus. Research in Veterinary Science 12, 272274.
Connan, R. M. ( 1975). Inhibited development in Haemonchus contortus. Parasitology 71, 239246.
Dobson, R. J., Waller, P. J. and Donald, A. D. ( 1990). Population dynamics of Trichostrongylus colubriformis in sheep: the effect of infection rate on the establishment of infective larvae and parasite fecundity. International Journal for Parasitology 20, 347352.
Donald, A. D., Morley, F. H. W., Waller, P. J., Axelsen, A. and Donnelly, J. R. ( 1978). Availability to grazing sheep of gastrointestinal nematode infection arising from summer contamination of pastures. Australian Journal of Agricultural Research 29, 189204.
Gatongi, P. M., Prichard, R. K., Ranjan, S., Gathuma, J. M., Munyua, W. K., Cheruiyot, H. and Scott, M. E. ( 1998). Hypobiosis of Haemonchus contortus in natural infections of sheep and goats in a semi-arid area of Kenya. Veterinary Parasitology 77, 4961.
Gordon, H. M. ( 1948). The epidemiology of parasitic diseases, with special reference to studies with nematode parasites of sheep. Australian Veterinary Journal 24, 1744.
Gordon, H. M. and Whitlock, H. V. ( 1939). A new technique for counting nematode eggs in sheep faeces. Journal of the Council of Scientific and Industrial Research 12, 5052.
Lindqvist, A., Ljungstrom, B. L., Nilsson, O. and Waller, P. J. ( 2001). The dynamics, prevalence and impact of nematode infections in organically raised sheep in Sweden. Acta Veterinaria Scandinavica 42, 377389.
Mansfield, M. E., Todd, K. S. Jr and Levine, N. D. ( 1977). Developmental arrest of Haemonchus contortus larvae in lambs given larval inoculum exposed to different temperatures and storage conditions. American Journal of Veterinary Research 38, 803806.
McKenna, P. B. ( 1973). The effect of storage on the infectivity and parasitic development of third-stage Haemonchus contortus larvae in sheep. Research in Veterinary Science 14, 312316.
Muller, G. L. ( 1968). The epizootiology of helminth infestation in sheep in the south-western districts of the cape. Onderstepoort Journal of Veterinary Research 35, 159193.
Ogunsusi, R. A. and Eysker, M. ( 1979). Inhibited development of trichostrongylids of sheep in northern Nigeria. Research in Veterinary Science 26, 108110.
Perry, B. D., Randolph, T. F., McDermott, J. J., Sones, K. R. and Thornton, P. K. ( 2002). Investing in Animal Health Research to Alleviate Poverty. ILRI (International Livestock Research Institute), Nairobi, Kenya.
Rossanigo, C. E. and Gruner, L. ( 1995). Moisture and temperature requirements in faeces for the development of free-living stages of gastrointestinal nematodes of sheep, cattle and deer. Journal of Helminthology 69, 357362.
Silverman, P. H. and Campbell, J. A. ( 1959). Studies on parasitic worms of sheep in Scotland. I. Embryonic and larval development of Haemonchus contortus at constant conditions. Parasitology 49, 2338.
Troell, K., Mattsson, J. G., Alderborn, A. and Höglund, J. ( 2003). Pyrosequencing analysis identifies discrete populations of Haemonchus contortus from small ruminants. International Journal for Parasitology 33, 765771.
Troell, K., Waller, P. J. and Höglund, J. ( 2005). The development and overwintering survival of free living larvae of Haemonchus contortus in Sweden. Journal of Helminthology 79, 18.
Waller, P. J., Rudby-Martin, L., Ljungstrom, B. L. and Rydzyk, A. ( 2004). The epidemiology of abomasal nematodes of sheep in Sweden, with particular reference to overwinter survival strategies. Veterinary Parasitology 122, 207220.
Waller, P. J. and Thomas, R. J. ( 1975). Field studies on inhibition of Haemonchus contortus. Parasitology 71, 285291.

Keywords

Phenotypic characterization of Haemonchus contortus: a study of isolates from Sweden and Kenya in experimentally infected sheep

  • K. TROELL (a1), C. TINGSTEDT (a1) and J. HÖGLUND (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed