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Participation of Trypanosoma cruzi gp63 molecules on the interaction with Rhodnius prolixus

  • Karina M. Rebello (a1), Livia A. Uehara (a1), Vítor Ennes-Vidal (a1), Aline S. Garcia-Gomes (a1) (a2), Constança Britto (a3), Patrícia Azambuja (a4), Rubem F. S. Menna-Barreto (a5), André L. S. Santos (a6), Marta H. Branquinha (a6) and Claudia M. d'Avila-Levy (a1) (a7)...


Trypanosoma cruzi is the causative agent of Chagas disease, a vector-borne disease. The parasite molecules involved in vector interaction have been little investigated. Metallopeptidases and gp63 molecules have been implicated in parasite adhesion of several trypanosomatids to the insect midgut. Although gp63 homologues are highly expanded in the T. cruzi genome, and are implicated in parasite–mammalian host interaction, its role in the insect vector has never been explored. Here, we showed that divalent metal chelators or anti-Tcgp63-I antibodies impaired T. cruzi adhesion to Rhodnius prolixus midgut. Parasites isolated after insect colonization presented a drastic enhancement in the expression of Tcgp63-I. These data highlight, for the first time, that Tcgp63-I and Zn-dependent enzymes contribute to the interaction of T. cruzi with the insect vector.

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This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (, which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

Corresponding author

Author for correspondence: Claudia M. d'Avila-Levy, E-mail:,


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